浓度

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

The results indicate seawater concentration has no effect on absorbency, pH, relative density, dry matter and water contents in Aloe vera leaves. While, seawater stress causes increased MDA and membrane penetration, decreased phytochroms, prolines and NO_3~--N. It is beneficial to plant growth when irrigation was done with 10% seawater, because of higher ratio of K~+/Na~+ and lower ratio of Na~+/Ca~(2+). In addition, the polysaccharide contents in Aloe vera leaves increase twice and triplication under 25% and 50% seawater irrigation respectively, the barbaloin contents increase over triplication in var. 75% seawater and over twice in var. 50% seawater and var. 100% seawater. However, when seawater concentration exceeds 25% the growth of plant was inhibited, biomass decreased, flowering time was deferred, Ca~(2+) content decreased caused by Na~+ accumulation in leaves.

结果表明：在海水胁迫下，库拉索芦荟全叶汁的吸光度、酸度、相对密度、总固形物和水分含量总体上受海水浓度的影响不大；但芦荟叶的丙二醛含量和质膜透性增加，叶绿体色素、脯氨酸和硝态氮含量减少；在10％海水浇灌下，芦荟叶细胞具有较高的K~+／Na~+比和较低的Na~+／Ca~(2+)比，对库拉索芦荟的生长有一定的促进作用；在25％海水和50％海水浇灌下，芦荟多糖含量提高了2-3倍；在75％海水浇灌下芦荟甙含量增加了3倍多，在50％海水和100％海水灌溉下，其含量也增加了2倍多；当海水浇灌浓度超过25％后，库拉索芦荟的生长明显受到抑制，生物量降低，抽苔期推迟，同时，海水浓度的增加也导致芦荟叶中累积大量Na~+，从而使得芦荟叶中Ca~(2+)含量降低，但Mg~(2+)并没有受到海水浓度的影响；用浓度低于75％的海水浇灌库拉索芦荟可以降低剖面深度在0-100cm之间的土壤盐度；其抗氧化保护酶中超氧化歧化酶的酶活力相对保持稳定，过氧化物酶的酶活力在10％海水浇灌下明显提高，随后则下降，而过氧化氢酶的酶活力则随海水浓度的升高而下降。

After Cryopreservation of embryo and larvae of manina clam Ruditapes variegata, the results indicate the survival rate of blastula embryo and vlegier are significantly better than that of trochophore and D-shaped larvae(P.05); the survival rate of blastula embryo is 42.3%(4.0M DMSO) and the effect of high sucrose density is better than that of low sucrose density with the same DMSO dendity.

对杂色蛤胚胎和幼虫的超低温冷冻保存发现，杂色蛤囊胚和面盘幼虫的保存复活率显著高于担轮幼虫和D形幼虫的复活率(P＜0.05)；杂色蛤囊胚的解冻复活率达42.3％(4.0M DMSO)，相同DMSO浓度条件下，高蔗糖浓度组保存效果好于低蔗糖浓度组，但差异不显著(P＞0.05)；在相同蔗糖浓度条件下，抗冻液中DMSO浓度较高组的保存效果好于DMSO浓度较低的组，但差异不显著(P＞0.05)。

The concentrations of TNF-αand IL-10 in peripheral blood increased significantly after CVVH.The concentration of NO decreased significantly after CVVH.There was no TNF-αin ultrafiltrate;IL-10 was detected little and NO was detected much in ultrafiltate.Compared with prefilter,the concentration of TNF-αin postfilter blood decreased significantly at 1h of CVVH and increased at the end of CVVH. Compared with prefilter,the concentration of NO in postfilter blood decreased significantly at 1h ,12h and the end of CVVH.

CVVH治疗后患者血清TNF-α、IL-10浓度与治疗前相比明显升高，而NO浓度与治疗前相比显著下降，超滤液中未能检测出TNF-α，仅检测到少量IL-10，但NO在超滤液中浓度较高；CVVH治疗1h时，静脉端血清TNF-α浓度明显低于动脉端，CVVH治疗结束时，静脉端血清TNF-α浓度明显高于动脉端；CVVH治疗1h，12h和结束时，静脉端血清NO浓度均明显低于动脉端。

Caudatum was studied. 0.05 mg/L could be the critical mass concn. of P. caudatum fitting to Cu(superscript 2+).Below this concn., the polysaccharide content in P. caudatum was increased in a small range with the increment of mass concn. of Cu(superscript 2+),then the growth and propagation of P. caudatum was strengthened and above this concn., the polysaccharide content was decreased significantly with the increment of mass concn. of Cu(superscript 2+), then the growth and propagation of P. caudatum began to be restrained obviously and the poison was more obvious when the concn.

结果］0.05 mg/L可能是大草履虫适应Cu(上标 2+)的临界质量浓度，低于此浓度时，大草履虫体内的多糖含量随着Cu(上标 2+)质量浓度增大而呈现小幅度的增高，大草履虫的生长繁殖得到增强，高于此浓度时，大草履虫体内的多糖含量随着Cu(上标 2+)质量浓度增大而显著地降低，大草履虫生长繁殖开始明显受到抑制，且浓度越高受毒害越明显。

Results:Biochemical indicators: the concentrations of K+, Na+ and Cl－ in the serum after the infusion are different with those before the infusion; other indicators don't have the significant difference. The mean infusion time of group A is longer than that of group B (p=0.006), while the dosis letalis of KCl doesn't have significant difference between groups (p=0.062); the concentrations of K+ in blood, Na+, Mg2+ and Cl－ in serum have the significant difference between groups; the urine volume and the concentrations of all the urine electrolyte indicators don't have the statistical significant difference between groups.

结果：生化指标：输入钾前后，家兔血清钾离子浓度升高，钠、钙及氯离子浓度降低，具有统计学显著性差异，p=0.000；全血钾及血清其他离子浓度变化，以p=0.05为标准，不具有统计学显著性差异。A、B两组间比较结果：A组输液致死时间长于B组(p=0.006)，致死输钾量无统计学显著性差异(p=0.062)；全血钾、血清钠、镁及氯离子浓度变化值具有显著差异，血清钾、钙、碳酸氢根离子浓度变化值无明显的差异；尿量和尿液各电解质浓度指标无统计学显著性差异。

The disclosed combined detection method for high-pure SO3 gas with SO2 as impurity comprises: constant-volume sampling at least 60Deg better 60-80Deg to avoid liquidation of SO3, absorbing with alkali solution to obtain total volume percentage concentration of high-pure SO3 gas and SO2; processing the absorbed liquid, using iodometric method to obtain SO2 volume percentage concentration and then the SO3 concentration.

一种高纯度SO 3 气体及其中杂质SO 2 气体浓度联合检测的方法，包括用吸收装置进行定容采样，然后用碱溶液吸收，得到高纯度SO 3 气体及其中杂质SO 2 气体的总体积百分浓度，再对吸收液进行处理后，采用直接碘量法测得SO 2 气体的体积百分浓度，将高纯度SO 3 气体及其中杂质SO 2 气体的总体积百分浓度减去SO 2 气体的体积百分浓度即为高纯度SO 3 气体浓度，其所述吸收装置进行定容采样时，是在至少60℃以上的热环境中进行的。

Time constants do not increase but the weights of the fittings concentration de pendently enhance as the external Ba2+ concentration augments. So the inac tivati on becomes faster and faster as the external Ba2+ concentration increases. Time constants decrease and the weights of the fittings concentration dependently enh ance as the external Ba2+ concentration augments when external Ba2+ concentratio n (10 or 100 μmol/L) is higher. So the inactivation becomes faster and faster. It is showed that Ba2+ can go deeper in IRK1-T from the surface of the ch annel a s external Ba2+ concentration increases.

三级指数拟合表明：细胞外Ba2+低浓度(1和3 μmol/L)时，Ba2+与K+相互竞争同一结合位点，随着Ba2+浓度的增加，时间常数不增加但拟合的权数却浓度依赖性增加，所以失活过程随Ba2+浓度的增加越来越快；细胞外Ba 2+高浓度(10和100 μmol/L)时，时间常数随Ba2+浓度的增加而减少，拟合的权数却浓度依赖性减少，失活过程也越来越快，说明Ba2+作用位点由通道的表面部位进入通道更深的地方。

Time constants do not increase but the weights of the fittings concentration de pendently enhance as the external Ba2+ concentration augments. So the inac tivati on becomes faster and faster as the external Ba2+ concentration increases. Time constants decrease and the weights of the fittings concentration dependently enh ance as the external Ba2+ concentration augments when external Ba2+ concentratio n (10 or 100 μmol/L) is higher. So the inactivation becomes faster and faster. It is showed that Ba2+ can go deeper in IRK1-T from the surface of the ch annel a s external Ba2+ concentration increases. CONCLUSION: Two diffe rent mechanisms for external Ba2+ blocking exist.

三级指数拟合表明：细胞外Ba2+低浓度(1和3 μmol/L)时，Ba2+与K+相互竞争同一结合位点，随着Ba2+浓度的增加，时间常数不增加但拟合的权数却浓度依赖性增加，所以失活过程随Ba2+浓度的增加越来越快；细胞外Ba2+高浓度(10和100 μmol/L)时，时间常数随Ba2+浓度的增加而减少，拟合的权数却浓度依赖性减少，失活过程也越来越快，说明Ba2+作用位点由通道的表面部位进入通道更深的地方。

Time constants do not increase but the weights of the fittings concentration de pendently enhance as the external Ba2+ concentration augments. So the inac tivati on becomes faster and faster as the external Ba2+ concentration increases. Time constants decrease and the weights of the fittings concentration dependently enh ance as the external Ba2+ concentration augments when external Ba2+ concentratio n (10 or 100 μmol/L) is higher. So the inactivation becomes faster and faster. It is showed that Ba2+ can go deeper in IRK1-T from the surface of the ch annel a s external Ba2+ concentration increases. CONCLUSION: Two diffe rent mechanisms for external Ba2+ blocking exist.

三级指数拟合表明：情绪细胞外Ba2+低浓度(1和3 μmol/L)时，Ba2+与K+相互竞争同一结合位点，随着Ba2+浓度的增加，时间常数不增加但拟合的权数却浓度依赖性增加，所以失活过程随Ba2+浓度的增加越来越快；情绪细胞外Ba2+高浓度(10和100 μmol/L)时，时间常数随Ba2+浓度的增加而减少，拟合的权数却浓度依赖性减少，失活过程也越来越快，说明Ba2+作用位点由通道的表面部位进入通道更深的地方。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。