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The reaction mechanism between nickel ion and butynediols in the electroless plating process was analyzed. The results show that the change of BOZ concentration is a quasi first-order reaction, the change of the nickel ion concentration is related to the BOZ density, and this change belongs to a higher order reaction. The reaction rate equation was determined as dC/dt=-8.585×10^(-4)+1.188×10^(-4)t-4.64×10^(-6)t^2+5.8×10^(-8)t^3-2.3×10^(-11)t^4. The activation energy of this process decreases as temperature increases, and it decreases with the BOZ concentration as 64-423 mg/L, it increases with the BOZ concentration as 123~240 mg/L. The S value is positively correlated to temperature, when the temperature is 80℃, the reaction rate and S value are the largest; while when the temperature is above 80℃ and BOZ concentration is over 160 mg/L, S value and the reaction rate decrease gradually.

结果表明,镀液中BOZ浓度的变化是准一级反应,镍离子的浓度变化与BOZ的浓度有关,属多级反应,其反应速率方程为dC/dt=-8.585×10^(-4)+1.188×10^(-4)t-4.64×10^(-6)t^2+5.8×10^(-8)t^3-2.3×10^(-11)t^4,反应体系的活化能E随温度的升高而减少,随BOZ浓度的变化而变化:当BOZ浓度在64~123mg/L时,E线性减少;在123~240mg/L时E线性增加。S随温度的升高而增加,当温度达到80℃时S值最大,反应速度最大;当温度超过80℃,BOZ浓度大于160mg/L时,蝎的值逐渐减少,反应速度降低。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果:体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;硒能增强重组VacA对胃癌细胞的抑制作用,使细胞分裂指数和集落形成率降低、S期细胞数量减少,G1期细胞总数增加;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

In nitrogen and phosphorus mixed pollution water, in thewater that the concentration of nitrogen is 21mg/L and the concentration of phosphorus is155mg/L, Eichharnia crassipes and Hosta ventricosa can grow normally. In the water that theconcentration of nitrogen is 210mg/L and the concentration of phosphorus is 31mg/L, thephysiological indicators of Hosta ventricosa is good but the physiological indicators of Eichhomiacrassipes is worse than the other treatment and the root of Eichharnia crassipes was rot when itwas planted in the 7th day, and on the 11th day the plants wilt symptoms, it noted that thistreatment affected the growth of Eichharnia crassipes.

在氮磷复合污染的水体中,凤眼莲和紫萼玉簪在氮浓度21mg/L,磷浓度155mg/L水体中都能够正常生长,而在氮浓度210mg/L,磷浓度31mg/L水体中,紫萼玉簪生长正常,而凤眼莲的生理指标比在其它处理中要差,并且凤眼莲在种植的第7天出现根部腐烂,11天时植株枯萎的症状,说明氮浓度210mg/L,磷浓度31mg/L复合污染在一定程度上影响植物的正常生长。

It was known that pyridine and Triton-x100 had a bad effect on the cell growth and lycopene production were studied. The results indicated that pyridine and triton-x100 inhibited cell growth and lycopene synthesis;β-ionone increased cell growth and lycopene synthesis when the concentration was 0.05 ml/L, but when the concentration became higher, there would be a decrease on the lycopene output; span-20 increased cell growth and lycopene synthesis effectively when the concentration reached 2.0ml/L, and the lycopene yield became 2.4×10~(-2) mg/L which was 3-fold than control.

研究了培养基中加入不同浓度的小分子效应物吡啶、Triton-x100、Span-20、β-紫罗酮、异烟肼对番茄红素产量的提高作用,结果显示培养基中加入很低浓度的吡啶(0.1ml/L)就会对菌体产生很强的毒害作用;triton-x100对类胡萝卜素的合成也有很强的抑制作用,试验的浓度为0.2ml/L到5ml/L,结果发现它对菌体的生长和番茄红素的合成都有抑制作用;β-紫罗酮在浓度为0.05ml/L时有很小的促进作用,而浓度升高就有了比较强的抑制效果;span-20在液体培养基中的加量为2.0ml/L时,对番茄红素的合成有很强的促进效果,产量达到了2.4×10~(-2)mg/L,比原始菌株产量提高了2倍,而浓度进一步增大就产生了很强的抑制效果。

The calculating result shows that the ununiformity of fiber distribution on the shell side can lead to the serious maldistribution of flow on this side, and significant channeling effect can be observed, the flux of practical hollow fiber module is much less than that of the ideal one.

该类间歇操作模拟计算结果表明,组件通量随料液浓度的升高而下降主要是由于水的蒸汽压随浓度的升高而下降,低浓度浓度极化的影响可以忽略;随着浓度的升高,浓度极化的作用越来越明显。

According to the ability of the field isolates of Gibberella zeae to grow on the PSA with varying carbendazim concentrations, three sensitivity levels of isolates were determined in vitro. The sensitive isolates could grow at 0.5 μg/ml, but could not grow at 100 μg/ml. The high resistant isloates could grow faster than R at 50 μg/ml, and also could grow at 100 μg/ml.No low resistant isolates, that could grow fast at 1.4 μg/ml but could not grow at 50 μg/ml, were found among the field isolates.

根据在0.5、1.4、50、100 μg/ml等不同浓度的含药PSA平板上能否生长,将玉蜀黍赤霉田间菌株对多菌灵的敏感性划分为:敏感、中抗和高抗等3个水平,其中S菌株在0.5 μg/ml浓度下能生长,但在≥1.4 μg/ml浓度下生长受到完全抑制;R菌株在1.4 μg/ml浓度下能快速生长,在50 μg/ml浓度下能缓慢生长,但在≥100 μg/ml浓度下不能生长;HR在≥100 μg/ml浓度下仍能生长。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果:经槲皮素处理的人结肠癌SW480细胞数量减少,部分细胞体积缩小,细胞膜完整,胞浆浓缩,核染色质固缩,细胞核碎裂,形成凋亡小体;MTT法检测显示当作用浓度为30μmol/L~90μmol/L时,槲皮素对人结肠癌SW480细胞的生长有抑制作用,其抑制作用随着作用浓度的增加和作用时间的延长而增强;流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期,大部分细胞被阻断于S期,随药物浓度的升高和作用时间的延长,G0/G1期细胞比例逐渐增加,S期细胞比例逐渐减少;酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9,随浓度的升高,MMP-2及MMP-9的分泌量减少;免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后,Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

Atmospheric pollen concentrations in a particular region vary markedly in response to geographical location, vegetational component, and various meterological parameters on many different time scales. Generally, during the 24-hour day, the highest pollen concentrations are observed at daylight, the lowest values are registered at night.

不同地区受其地理位置、植物组成和气候等多种因素的影响,空气花粉种类和浓度的变化模式有所不同,一般情况,一个地区白天空气花粉浓度高,夜晚浓度低;春夏季花粉浓度高,冬季花粉浓度低;春夏季乔木类植物花粉浓度高,而秋季草本类植物花粉浓度高。

The time-activity of workers was recorded manually during sampling, and the average exposure levels of nanoparticle mass concentrations and nanoparticle number concentrations to workers were calculated from the time-activity pattern and the mapping data. In the iron foundry plant, the worker with the highest exposure level was the crane driver whose average exposure levels of nanoparticle number concentrations and nanoparticle mass concentrations were found to be 5.17x104 ?q/cm3 and 11.39 ?慊/m3, respectively.

为了解工作人员的质量暴露浓度,本研究在采样的过程中利用人工的方式将工作人员的时间-活动模式加以记录,结合此时间-活动模式以及奈米微粒质量浓度分布图,可以推估出工作人员的平均奈米微粒数目浓度与质量浓度暴露量,其中铸造厂暴露浓度最高的工作人员为天车吊桶作业人员,其奈米微粒平均暴露数目浓度与暴露质量浓度分别为5.17x104 #/cm3、11.39μg /m3。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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