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The universal primers of ITS1 and ITS4 for fungi were used in amplification of genomic rDNA sequences. A target fragment was obtained by ITS-PCR. Then, the fragment is cloned into the vector of pMD18-T before sequencing. The result of sequencing showed that the fragment is 692 bp in length. The results of Blast in Genbank and the analysis of fungal rDNA systemic development show that the relation between this fragment to the fungi of Sporisorium is closest, while the relation between it to the fungi of Ustilago is much far. It suggests that the name of Ustilago scitaminea for sugarcane smut isn't consistent to the result of rDNA systemic development analysis. It still needs more experiments to support this hypothesis.

用真菌rDNA序列分析的通用引物ITS1和ITS4,通过ITS-PCR得到目的片段,克隆在pMD18-T载体上后进行测序,结果显示该片段的长度为692 bp,与Genebank中已报道的真菌序列进行Blast和系统发育树分析,表明所获得的序列与Sporisorium属真菌具有更高的亲缘关系,而与Ustilago属真菌的亲缘关系较远,这与一直沿用的甘蔗黑穗病菌的命名似乎并不吻合,有待进行更多的研究以证实。

Therefore, local wave decomposition technology was introduced into the logging date extrapolation method constrained by time-phase model to gain the seismic instantaneous phase. The relationship of logging and seismic data was established through the wellside strata correlation. High resolution attributes inversion profiles were generated through the extrapolation of logging constrained by equiphase surface established based on seismic data.

为此,在时间-相位模型约束的井资料外推方法中,引入局域波分解技术来求解地震瞬时相位;通过井旁地层对比,建立测井资料与地震资料之间的联系,把测并信息在地震资料建立的等相面约束下外推出去,以获得高分辨率的属性反演剖面。

Secondly, the genomic DNA is extracted from Zostera marina L. and then amplified by PCR with two oligonucleotide primers synthesized to obtain the partial nucleotide sequences of the Na~+/H~+ antiporter gene. The Na~+/H~+ antiporter gene clone was obtained after a series of manipulation: purificatioin of PCR product, ligation and transformation of recombinant plasmids, blue-white selection and so on. DNA sequence analysis shows that its DNA sequence is highly homologous with the genomic DNA sequence of E.

其次,通过提取大叶藻基因组DNA,根据设计的简并性引物进行PCR扩增,纯化PCR产物,连接,重组质粒转化,蓝白斑筛选等操作,并测序,提交测定序列到NCBI进行BLASTN,结果表明:该序列与大肠杆菌的基因组序列相似性较高,而与其它物种的Na~+/H~+逆向转运蛋白基因没有相似性。

The sizes of the self-assembly micelles of the amphiphilic block copolymers and the effects werestudied by dynamic light scattering and UV-visible spectrophotometer. The micelle sizes werelargely influenced bythe hydrophobic chain content in the copolymer, the properties of the solvent and the copolymer concentration in the organic phase. The morphology of the micelles was investigated by transmission electron microscopy. Theresults showed that the micelles appeared spheres with inner core and outer shell. The critical association concentrations were determined by pyrene monomer fluorescence probe technology. The degradability of copolymer was studied by the loss of the intrinsic viscosity in the degradation process. The results indicated that the degradation rate of PECL was slower thanthat of PELLA and PEDLLA. The stability of the copolymer micelle dispersion was examined by measuring the critical flocculation concentration, which gradually decreased with increasing the content of thehydrophobic chain in the copolymer. The rheological results showed thatthe viscosity ofthe micelle dispersion with higher content of PEG segments was firstly decreased and then increased with the increasingthe temperature. Otherwise, the variation of viscosity was irregular. The viscosity of the micelle dispersion increased with the increase of the electrolyte (Na 2SO 4) concentrations. Polymer micelles paclitaxel was prepared by self-emulsification/solvent evaporation method and solid dispersion technique with amphiphilic block polymers as the carrier material.

运用动态光散射和紫外分光光度计研究了两亲性嵌段共聚物自组装胶束的粒径及其影响因素,结果表明,自组装胶束的形成机理受制备方式的控制,胶束的粒径随着共聚物相对分子质量或疏水嵌段相对分子质量和有机相中共聚物浓度的增大而增大,随着有机溶剂的水溶性的增强而减小;采用透射电镜观察胶束的形态结构,发现胶束呈具有核壳结构的均匀球形;采用芘荧光探针法测两亲性嵌段共聚物的临界聚集浓度;通过降解过程中共聚物特性粘度的变化研究两亲性共聚物的降解性能,发现与PELLA和PEDLLA的相比,PECL 降解速率较慢;随着共聚物中疏水嵌段含量的增大,纳米分散液的稳定性逐渐下降;PEG含量高的PEDLLA纳米分散液随着温度的升高,纳米分散液的粘度先下降然后增大,反之,没有规律性,且随着电解质Na 2SO 4浓度的增大,体系粘度增大。

Companies with strong technical strength, advanced production technology and sophisticated processing and post-mortem test equipment, for the majority of users with high-quality products.

公司凭借雄厚的技术力量、先进的生产工艺、精良的加工及验测设备,为广大用户提供高质量产品。

This design has introduced performance and characteristic of the FX2N PLC machine, and a kind of low cost , high accuracy , microminiaturization , digital display taking it as the core one agitates the control system.

分类: PLC控制,自动化,测控技术论文编号:ZD344 论文字数:16730,页数:53,有开题报告,任务书,文献综述,CAD图摘要:本次设计介绍了FX2N PLC机的性能及特点,及以其为核心的一种低成本、高精度、微型化、数字显示的掺混控制系统。

The genetic behavior of these QTLs were investigated accordingto the performance of backcrosses.

5对以IR24为遗传背景的一套籼型渗入系进行了三地两年的详细考察,初步确认了各渗入系中所携带的渗入片段对抽穗期、株高、穗数、实粒数、总粒数、千粒重、单株重和结实率等性状的影响,并初步定位了11个重要农艺性状和产量性状QTLs,估算了这些QTLs的遗传效应,并在回交和测交中进一步确定了这些QTLs的遗传行为,讨论了渗入系在QTL精细定位与杂种优势机理研究中的应用。

The trick to making the study meaningful was making sure the test subjects didn't know what was going on, Dunning said, noting that the generally high IQ of Cornell students made cheating a real possibility.

达宁说使研究结果有意义的关键是保证受测者不知道测试的真正目的,尤其科内尔学生们的智商普遍较高,欺骗研究人员的可能性不是没有。

Bengbu Gaoling Sensing System Project Co., Ltd is a hi-tech enterprise in Bengbu High/New Technical Developing Zone specializing in the research, development and production of all kinds of series sensors and automatic metering and measuring system.

蚌埠高灵传感系统工程有限公司是蚌埠市高新技术开发区的高新技术企业,企业拥有丰富的设计生产经验和专业的生产检测设备,专业研究、开发、生产各种系列传感器及自动化计量、测控系统。

cyclic redundancy check code is a very important cyclic code,the coding and decoding methods are simple,easy to realize.it is strong to detect error,the probability of misjudging is nearly zero.it is a method with extremely high efficiency in controlling errors,which can fulfill the requirement of reliable information transmission in communication systems.it has got very extensive application in observing and controlling and data communication.this paper introduces the encoding and decoding principle of crc in detail,analyzes the feasibility of crc by dsp,and gives out the design proposal and flow chart according to the principle,which is very useful in applications.

摘 要:循环冗余校验码是一种重要的循环码,编码和解码方法简单,容易实现,检错能力强,误判概率几乎为零,是一种效率极高的差错控制方法,可以满足通信系统可靠传送信息的要求,在测控及数据通信中得到了非常广泛的应用。详细介绍了循环冗余校验的编解码原理,分析了用dsp实现crc的合理性,最后给出了根据校验原理实现的设计思想及流程图,具有一定的实用价值。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。