测定法

Change in the concentration of endogenous IAA was measured with enzyme-linked immunosorbent assay, and the distribution of IAA-binding protein in the protoplasts in the cambial region was tested with an immuno-fluorescence technique, being improved by the authors, during the cambial active period in Broussonetia papyrifera L.

构树（Broussonetia papyriferaVent。）形成层活动周期中用酶联免疫吸附法测定了形成层区域的内源IAA浓度的变化，并用自行发展的免疫荧光检测方法测定了由此处生活细胞制得的原生质体中IAA结合蛋白的分布。

The MTT assay was used to detect the viability of cells. Flow cytometry and laser confocal microscopy were used to identiyfy the cell cycle and apoptotic morphology after cells treated with LAO. RESULTS: An L-amino acid oxidase was successfully purified from the venom of bungarus fasciatus.

结果： 通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，L－氨基酸氧化酶的表观分子量通过SDS-PAGE测定约为60 kD，通过凝胶过滤测定分子量为70 kD.L－氨基酸氧化酶作用于HUVCE细胞12 h后，细胞的生长明显受到抑制，L－氨基酸氧化酶作用的半数抑制量为2.8 mg/L。

Combined with optics, calorifics, electrics and acoustics, PA theory and PA application need deeply studied.

光声光谱的理论和应用，还需深入研究和开拓发展。光声光谱法适用性广，对于传统吸收光谱难以测定的不透明、高反射、高散射样品，也可以方便地测定。

The influence on physiques of Camponotus japonicus which were kept indoors in winter was studied. Their survival rate was counted. The activity of SOD and POD was tested by spectrophoto-meter.

研究室内过冬对日本弓背蚁生活力的影响，统计法测定了其存活力的变化，分光光度计比色法测定了野生蚁及低温处理蚁超氧化物歧化酶、过氧化物酶等指标。

Lipase mediates esterification between caproic acid (0.6mol/L) and ethanol (0.6mol/L) in n-heptane at 40℃. The decrease in caproic acid is measured by titration and 1 unit of lipase synthetic activity is defined as 1 μmol caproic acid consumed per min.

在40℃下，脂肪酶催化己酸(0.6mol/L)与乙醇(0.6mol/L)在正庚烷中进行酯合成反应，通过滴定法测定剩余的己酸，1个脂肪酶有机相合成酶活单位定义为：在测定条件下每分钟消耗1μmol己酸的酶量。

The optimal determining conditions were obtained: adding quantity of a monolayer medium 15ml, the suspension concentration of Micrococcus luteus 109 cfu/ml, the adding quantity of Oxford Plate 100μl, agar concentration 1%, the concentration of Na2HPO4 1％, and the pH of the medium adjusted to 7.0. The medium was optimized by single factor and the orthogonal experiments on the basis of the centrifugate of distiller's grains. The optimal composition was obtained as follows: sucrose 0.6, peptone 0.1, yeast extract 0.2, K2HPO4?

以管碟法为基础，对影响Nisin效价测定的各种主要因素进行了分析，并得出了Nisin效价测定的最佳条件：采用单层培养基（每个平板加量为15ml），检测培养基中Na2HPO4·12H2O浓度改为1％，菌悬液浓度109cfu/ml，不加吐温20，琼脂浓度1％，培养基pH7.0，牛津杯加液量100μl ，30℃培养24小时。

Effect of different surfactants on the UV intensity of Al(superscript 3+)-MXFX have been studied. Is Cetyl Trimethy-lammonium Bromide can increase the UV intensity of Al(superscript 3+)-MXFX is discovered. Based on this reaction, the reaction conditions in detail and proposed a simple, rapid and sensitive new method for the determination of MXFX by UV method is inve stigated. This method has been applied for the determination of MXFX in tablet with satisfactory results.

通过研究不同表面活性剂对Al(上标 3+)-莫西沙星体系紫外特性的影响，发现十六烷基三甲基溴化铵对该体系有显著的增敏作用；系统研究了CTMAB-Al(上标 3+)-MXFX反应体系的紫外特性，利用这一反应体系，建立了简单、快速、灵敏的测定莫西沙星的紫外分光光度法，选择了最佳实验条件，并将此方法用于莫西沙星片剂的测定，结果满意。

These results were consistent with those obtained via gas chromatographic method.

这些测定结果与传统气相色谱法的测定结果相吻合。

Then the levels of TC, TG, LDLC and TAFI were measured by chromogenic assay.

用全自动生化仪测定血脂系列，发色底物法测定血浆TAFI的活性。

Methods The model of SAH was set up by injecting blood two times via cisternal after occlusion of bilateral carotid in rabbit. The food-intake and behavior of experimental animals were under observation at various stages of experiment. The content of ET in blood and CSF was tested by radioimmunoassay, and the NOx- by HNO3 redutase method. Regional cerebral blood flow was measured by hydrogen clearance method.

方法采用双侧颈动脉结扎和枕大池二次注血制成兔SAH模型，观察SAH前后动物进食量和神经功能改变，用放射免疫方法和硝酸还原酶法分别测定血液和脑脊液中ET和NOx-含量，以氢清除法测定局部脑血流量。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。