测定法

METHODS: The 144 BALB/c male mice were abdominally irradiated with 1150 cGy 60Coγray in 1-3 h, and then they were injected intestinal RNA from normal rats on jejunum. On day 1, 3 and 5 after irradiation,the mice were sacrified after anesthesia for determining endotoxin in blood and bacterial metathetic rate, and intestinal crypt survival rate.

选用BALB/c雄性小鼠144只，用1150 cGy 60Coγ射线进行腹部一次照射，于照后1~3 h采用局部肠腔扩张注入法给小鼠空肠肠腔内注入正常大鼠小肠RNA，分别于照后1，3，5 d麻醉后解剖，取血，测定内毒素含量；取肠系膜淋巴结，测定细菌移位率；取空肠段，计数肠腺存活率。

METHODS: The 144 BALB/c male mice were abdominally irradiated with 1150 cGy 60Coγray in 1-3 h, and then they were injected intestinal RNA from normal rats on jejunum.

选用BALB/c雄性小鼠144只，用1150 cGy 60Coγ射线进行腹部一次照射，于照后1~3 h采用局部肠腔扩张注入法给小鼠空肠肠腔内注入正常大鼠小肠RNA，分别于照后1，3，5 d麻醉后解剖，取血，测定内毒素含量；取肠系膜淋巴结，测定细菌移位率；取空肠段，计数肠腺存活率。

The determination of cytokinin levels is a primary and basic approach in the cytokinin studies. The Amaranthus betacyanin bioassay was modified for the rapid and convenient determination of cytokinin activity. Meanwhile, the procedures for quantitative analyses of cytokinins by HPLC and ELISA were estabolished, and these gurantee the analyses of cytokinin levels with high precision.

在细胞分裂素的活性测定中，通过改进尾穗苋苋红素合成法建立了一种简便、准确的生物试法，同时还建立了根据物理化学和免疫学原理而测定细胞分裂素的HPLC和ELISA方法，使得细胞分裂素的定量更加准确。

Objective To establish a method for determination of oleanolic acid in Cortex Aralia Elatae by HPLC.

目的 建立HPLC法测定龙芽楤木药材中齐墩果酸的含量测定方法。

After ferment, determined the consistency of the aweto; adjust the proportion of herbs by orthogonal test, determined the amrita-chun content of mycelium and amylose content of ferment liquid.

发酵后，测定冬虫夏草菌丝体的浓度；在此提取液浓度下，用正交试验法调节这些中药用量的配比，对菌丝体中的甘露醇及发酵液中的多糖含量进行测定。

There are two methods to detect and quantitative analysis Neu5Ac, one is high performance liquid chromatography with the use of Aminex87H column, the other one is barbituric acid method.

对Neu5Ac的检测和定量也有两种方法，一种是利用Aminex87H酸性糖基柱，通过高效液相色谱测定；另一种是硫代巴比妥酸法测定。

The patients with bisectional prostate hypertrophy (BHP, 415 cases) and prostate cancer (PCa, 225 cases) were diagnosed by pathobiology, and whose levels of PSA, and fPSA were examined in serums by ELISA.

病理诊断为415例良性前列腺增生和225例前列腺癌，利用酶联免疫法测定患者血清中的PSA、游离PSA，利用经直肠超声测定前列腺体积，并计算出f/tPSA及PSAD进行统计学分析。

Using the technology of productive performance testing and BLUP animal model,the swines' backfat thickness and the age had been tested successively on Landrace、Yorkshire and Duroc breeding pigs when they were at100kg in past three years.

采用种猪性能测定技术和多性状动物模型BLUP法，连续3年对四川3个外种猪(长白、大约克夏、杜洛克)100kg体重活体背膘厚和日龄两个性状进行了性能测定和遗传进展研究。

Methods Acid dye colorimetry was used in the determination with bromocresol green buffer solution as the color developing agent with absorbance was determined at the detection wavelength of 417 nm.

目的 建立脑脉通有效部位中总生物碱的含量测定方法。方法采用酸性染料比色法，以溴甲酚绿缓冲液作为生物碱的显色剂，测定样品溶液在417 nm波长处的吸收度。

Guangdong college of pharmacy,guangzhou,guangdong 510006,china;henan university of traditional chinese medicine,zhengzhou,henan 450008,chinaabstract:objective to establish a method for the determination of the content of total alkaloid in nao mai tong recipe.methods acid dye colorimetry was used in the determination with bromocresol green buffer solution as the color developing agent with absorbance was determined at the detection wavelength of 417 nm.results the calibration curve of the absorbance was linear with the concentration of ligustrazine in the range of 0.031～0.153 mg/ml(r=0.9998).the average recovery of ligustrazine was 100.95％,rsd=1.75%.

作者单位：广东药学院中药化学与分析教研室，广东广州 510006；河南中医学院，河南郑州 450008 目的建立脑脉通有效部位中总生物碱的含量测定方法。方法采用酸性染料比色法，以溴甲酚绿缓冲液作为生物碱的显色剂，测定样品溶液在417 nm波长处的吸收度。结果对照品川芎嗪质量浓度在0.031～0.153 mg/ml的范围内与吸光度呈良好的线性关系，r=0.9998。平均回收率为100.95％，rsd为1.75％。精密度和稳定性良好。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。