测定法

In this research, swainsonine contents of three Oxytropis species were determined by the high performance liquid chromatography coupled to mass spectrometry. The population genetic diversity was also studied and the swainsonine related molecular markers were screened by ISSR. In vitro fungi cultures from the individual plant of O. glabra were performed and microbiological morphology of the colony and the conidiophore were investigated. The 5.8S rDNA/ITS sequences of fungi amplified from total DNA in both plants and fungal endophytes were studied. These sequences were analysed and compared, and they were also compared to the 5.8S rDNA/ITS sequence of fungal enduphyte Embellisia sp. L12 from the O.lambertii, O.sericea and A.mollisimus distributed in USA.

本研究采用高效液相色谱-质谱联用法测定了三种棘豆植物的苦马豆素含量；通过ISSR分子标记技术对三种棘豆种群遗传多样性及其苦马豆素相关标记进行了研究；将小花棘豆植物单株进行体外真菌培养，研究了菌落和分生孢子的微生物形态学特性，从植物总DNA和相同植株培养的真菌总DNA扩增真菌的5.8SrDNA/ITS区，进行了DNA序列分析与排序；联系单株小花棘豆的苦马豆素含量测定和内生真菌ITS序列分析结果，与从美国分布的二种棘豆(O.lambertii、O.sericea)和一种黄芪(A.mollisimus)中分离的内生真菌埃里砖格孢属Embellisia sp.L12株得到的结果进行了比较。

Methods:Using cell culture technique,the inhibitory effects of NCTD on 3AO and AO cells were measured by way of crystal violet dyeing.The alterations of cell cycle induced by NCTD were analyzed with flow cytometry.

利用细胞培养技术，以结晶紫染色法测定NCTD对卵巢癌3AO和AO细胞的抑制作用，并利用流式细胞仪测定分析NCTD作用下两种肿瘤细胞的细胞周期变化。

Histopathological changes of lung tissue were observed under light microscope. The protein expression of MMP (MMP-2, MMP-9) and TIMP (TIMP-1) in lung tissue were determined by immunocytochemistry. RESULTS: Decompensate respiratory acidosis (pH =7.20±0.04, PaCO2 =7.84±0.15) developed in group B.

用ELISA法测定大鼠血清MMP-2、MMP-9、TIMP-1活性及NE活性；弹力纤维染色标本进行弹性纤维相对含量的测定；免疫组化方法检测肺组织中MMP-2、MMP-9及TIMP-1的表达及光镜下进行病理学观察。

The theory about electromotion effect on the interface between gas and water, formation of ion wind during corona discharge and double film absorption is used to explain the effect of simultaneously desulfuration and denitration in the gas and water phases.

在汇总和分析了羟自由基的测定方法的基础上，以产生羟自由基的Fenton 反应为研究对象，建立了以溴酚蓝为显色剂的基于比色法的放电条件下羟自由基测定体系，实验证明电晕放电下水中产生的羟自由基强化了烟气中的脱硫脱硝效果。

Leco oxygen determinator is adopted to determine the surface oxygen content and chemical assay is adopted to determine the total oxygen content.

所有牌号合金焊粉生产、包装严格控制制氧含量， Leco 定氧仪测定表面氧含量，化学分析法测定总氧含量。

Potassium dichromate was replaced by ceric sulfate as oxidant to determine the COD in this study.

采用硫酸高铈作为氧化剂来测定难降解垃圾渗滤液的COD，测定结果与重铬酸钾法进行比较。

Nevertheless the dissociation constant data of HPPA were not found in the literature.

然而文献上未见HPPA离解常数的报道，为此用pH计测定酸碱滴定法测定了HPPA的离解常数pKa1和pKa2，并进行不确定度评定。

MethodsBasket-stirring method was used to determine the dissolution rate by determining the dissolubility of puerarin at different time.

采用转篮法测定两者的体外溶出度，以葛根素不同时间溶出度为测定指标。

The method of gas chromatography- electron capture detection for the determination of N_2O in air,liquefied air and liquefied oxygen was studied and set up.

建立了一种用电子捕获检测器气相色谱法测定大气及空气分离装置液空、液氧中N2O的测定方法。

Determination of the equilibrium constant of a coordinated compound.

四、铁离子与磺基水扬酸配合物的组成和标准稳定常数的测定，了解用比色法测定配合物的组成和配离子稳定常数的原理和方法，掌握分光光度计的使用及有关试验数据的处理方法。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。