测定法

Methods The methods of DPPH , pyrograllol auto-oxidation and salicylicl acid were used in order to get the scavenging quality of DPPH free radical , superoxide free radical O2-.

方法采用DPPH法、邻苯三酚自氧化法、水杨酸法，分别测定缬草醚酯提取物清除DPPH自由基、超氧阴离子自由基和羟自由基的能力。

At last,the optimized formula composition and technique of preparation were obtained by orthogonal design,and long-circulating praziquantel liposome was prepared.

在建立吡喹酮含量测定方法的基础上，通过葡聚糖凝胶柱色谱法、显微镜法、激光散射法等定性及定量方法考察脂质体的包封率、微粒外观、粒径及其分布等指标。

The 9 kinds of elements, 6 kinds of fatty acids and 15 kinds of amino acids in the reddish fish scale were studied by ICP-AES, GC-MS and HPLC in this paper.

用等离子体发射光谱法、气相色谱-质谱联用分析法和高效液相色谱法分别测定了金线鱼鱼鳞中9种元素、6种脂肪酸和15种氨基酸的含量。

Methods: The study was designed to observe SS and influence of serum CD_4~+、CD_8~+、IL-2、IL-6、TNF-α, splenic lymphocyte transformation rate, the expression of SS、SSTR-2 in spinal cord、PAG、NRM and hypothalamus the coexistence of SS and enkephalinergic neuron in back corner of spinal cord, the coexistence of SS and 5-HT neuron in NRM by using methods of behavioral nociceptive threshold test, measure of foot's cubage, flowcytometry, MTT, radioimmunity, HPLC-ECD, immunohistochemistry, laser confocal microscope on FAC-induced hyperalgesia.

以弗氏完全佐剂诱发的炎症痛模型大鼠为研究对象，应用行为学痛阈观察、足跖容积测定、流式细胞检测法、MTT法、放射免疫法、高效液相电化学法、免疫组织化学技术法、激光共聚焦技术等方法，观察SS和电针对痛阈、大鼠CD_4~+、CD_8~+、IL-2、IL-6、TNF-α水平和脾淋巴细胞转化率等指标的影响，以及SS、SSTR-2在脊髓和PAG、NRM及下丘脑部位的表达、在脊髓SS与脑啡肽能神经元的共存现象及在NRM中与5-HT能神经元的共存情况。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

Methods Hyodeoxycholic acid and bilirubin in Calculus Bovis were separately identified by TLC, and the cholic acid content was determined by TLCS with the scan wavelength of λ=380 nm,λ=650 nm.

①用薄层色谱法分别鉴别猪去氧胆酸和胆红素；②用薄层扫描法测定胆酸含量，扫描波长：λ=380nm，λ=650nm。结果薄层色谱法可以区分体外培育牛黄和人工牛黄。

9 healthy adult Holstein cows were selected as experimental cows and they were divided into 3 groups according to different milk lactations. Individual tracking method was used to observe and record the amount of Diptera insects. The blood was took on the tail vein under the aseptic condition and then the serum was extracted. The level of ACTH, COR, PRL, GH and NE in the serum were detected by the method of radioactive immunoassay method and fluorescence spectrophotometry.

方法］选择9头成年健康的荷斯坦奶牛作为试验牛，按不同泌乳阶段分3组，采用个体跟踪法观察并记录侵袭试验牛双翅目昆虫的数目，自尾静脉无菌采血，提取血清，采用放射性免疫法和荧光分光光度法测定血清中AGTH、COR、PRL、GH和NE的水平。

In order to understand the lead and mercury content in Baby, sedative Chinese herb drugs, 179 preparations and 52 herbs of Chinese drugs were analyzed. Samples were prepared by Ash determination in lead and sulfuric acid-nitirc acid digestion in mercury, then determined by AAS.

将市售小儿惊风散类制剂179件及其药材52种，分别以灰化法及硫酸硝酸法处理后，利用原子吸光光度法(Atomic Absorption Spectrometry；AAS)测定铅及汞之含量。

Traditionally,endogenous amino acids are determined by feeding nitrogen-free diets,the regression method or complete digestibile protein or intravenous injection AAs,but these techniques contain both des...

在内源氨基酸的测定方法中，以传统方法(无氮日粮法、回归外延法和完全可消化蛋白源或静脉灌注平衡氨基酸法)最为常用，但存在种种缺陷。

This paper disuissed the deduction of the focal distance f using the Gauss method and the total yoke method in an imperfect situation,namely a situation where thin lens merely involve light core errors.

在普通物理光学实验中，在理想状态下测定薄透镜焦距常采用的方法有高斯法、共扼法、自准直法等。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。