测定法

GC-MS is used to determine content of free TDI, reaction kinetics is analysed by chlorhydric acid/n-dibutyl amine titration -NCO method and DSC, so the system activation energy is calculated.

采用气相色谱-质谱法测定其游离TDI含量，用盐酸-正二丁胺滴定-NCO法和DSC法相结合，分析反应动力学规律，计算体系表观活化能。

Morphometry of numerical density and electron probe X-ray quantitative microanalysis were adopted to measure the alterations in the number and calcium content of chromaffin granules in adrenal medulla cells during the agonist treatment. Secretion of epinephrine was detected by the high performance liquid chromatography.

运用细胞立体形态计量法计算分泌过程中的嗜铬颗粒数目的变化，运用电镜X射线显微分析法测量分泌过程中嗜铬颗粒内钙含量的变化，并运用高压液相色谱分析法测定离体细胞在激动剂作用后的肾上腺素分泌情况。

The molar ratio of sulfate ions to carboxylate ions is determined by conductometric titration method and the molecular weight is tested by high performance gel permeation chromatography. The precision of these two methods is also studied. To improve the detection sensitivity, guanidine hydrochloride is used as the best derivation reagent of 911. A systematic research is done on the optimum derivatization condition of 911 and Guanidine hydrochloride. The M /G ratio of 911 has direct relation to its biological activity.

论文首先对911的基本理化性质进行了系统的分析，以电导滴定法和高效凝胶渗透色谱法分别测定了分子中硫酸根羧酸根的比值和911的分子量，并探讨了两种分析方法的精密度。911分子没有紫外吸收，为提高检测灵敏度，本文采用荧光标记法，通过对三种荧光试剂与几种寡糖和多糖衍生效果的综合比较，最终确定了盐酸胍为最佳衍生试剂，并以911为代表详细探讨了衍生化反应的最优条件。911糖链结构与褐藻胶主链结构类似，以甘露糖醛酸和古罗糖醛酸的嵌段形式存在，分子中M/G比值与911的物理化学性质和生物活性有直接关系。

The measuring methods for diacetyl content in beer including EBC method, GC method and chemical radiation method were optimized and compared.

对EBC法、气相色谱法和化学发光法测定啤酒中的双乙酰含量进行了优化和比较。

Nepalensis wall polysaccharide was prepared with hot water extraction,ethanol precipitation,dialyzation,and lyophilization.the crude polysaccharide was separated by deae-cellulose chromatography and purified by sephadex g-200 chromatography.the inhibit effect of the polysaccharide on lipid peroxidation of mice liver was conducted by tba method.the model of liver-injury induced by carbon tetrachloride（ccl4was used to validate the variety of the realated ast.results onefold ingredient polysaccharide was obtained after isolated and purified.conclusion the ingredient can remarkably inhibit lipid peroxidation of rat liver and significantly reduce activity of ast.

用热水提取，乙醇沉淀提取粗多糖，再经sevag法去除蛋白，透析去除小分子物质，冷冻干燥得楮头红多糖粗品；采用deae-52纤维素柱层析和sephadex g-200凝胶柱层析进行分离纯化；用tba法考察多糖的抗脂质过氧化作用；采用ccl4急性肝损伤模型，测定其血清中ast变化。结论获得一个楮头红多糖组分。tba法表明该组分对小鼠自发性脂质过氧化具有显著的抑制作用；在保肝试验中，给药组能明显抑制ast的升高，对肝损伤有一定的保护作用。

By the method of dilatometric change,combining with microscopic test and hardness measurement,different expanding curves of continuous cooling transformation were measured at different cooling rate,and CCT diagram was obtained by using DIL805A thermal expansion fast phase transition instrument.

利用膨胀法，结合金相法和硬度法，在DIL805A热膨胀快速相变仪上测定了该钢在不同冷速下连续冷却时的热膨胀曲线，获得了该钢的连续冷却转变曲线；研究了该钢连续冷却过程中奥氏体转变过程与组织变化。

In this research,taking Wistar rats models of SCI made by Allen"s as objects of study ,treatment is taken by Jia Ji electropuncture and dexamenthasomein 6h and 12h after SCIin T_(11_12) segments ;model rat"s motor and sensory function are studied BDNF ,NGF,BDNFmRNA ,BCL-2,BCL-2mRNA,Bax are studiedbyimmunohistochemistry ,insituhybridization ,optic ,electro n microscope in different time .

本课题以Wistar大鼠经Allen's法制备脊髓撞击伤模型，对胸11～12损伤节段采取不同时间进行夹脊电针治疗，即伤后6h和12h接受夹脊电针治疗和地塞米松治疗，以联合行为评分法测定不同时间损伤大鼠的运动功能，感觉功能，以免疫组化法，原位杂交法，光镜技术，电镜技术，观察不同时间点伤段脊髓的BDNF，BDNFmRNA，NGF，BCL-2，BCL-2mRNA，BAX表达。

Under inhibit tumor fall increment andalert confession The objective of this experiment is to prove the antitumor effect and its mechanism in vivo of Erysimum cheiranthoides. In this study we observe the ratio of inhibitory of mice bearing S180 and the effect of prolonging lives of mice bearing H22;the effect on immune function of mice bearing S180;the phagocytosis of MO cells; measuring serum TNF of mice bearing S180 with ELISA; the expression level of p53 in tumor tissue with ICH and the change of tumor cells shape with pathology test.

实验采用S_(180)、H_(22)两种瘤株，以昆明利，小鼠为研究对象，对体内的抑瘤作用、生命延长作用进行研究；对S_(180)荷瘤小鼠免疫功能影响的研究；采用碳粒廓清法研究该药对巨噬细胞吞噬功能影响的研究；采用ELISA法检测S_(180)荷瘤小鼠外周血TNF含量；采用免疫组化法测定S_(180)小鼠瘤组织中p53蛋白的含量并观察瘤细胞的病理形态。

Quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E wereisolated from the flower of Acanthopanax senticosus. Searching bioactive natural compound and establishing quality evaluation standard arefocused on this experiment. Using cold immersing of 70% methanol to extract from flower ofAcanthopanax senticosus and integrating macroporous resin, silica gel column chromatography,sephadex gel to abstract. We obtained five monomer compounds (compoundⅠ-compoundⅤ),quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E. That five compoundswere determined by HPLC in eight parts of Acanthopanax senticosus (flower, root, leaf fruit,shoot, new shoot, phloem of shoot, skin of shoot), which provide science bases for establishingquality evaluation system and settle infarctate foundation.

本文以从刺五加花蕾中寻找具有重要生物活性的天然化合物和建立质量评价标准为主要目的，对其化学成分进行研究，采用70％甲醇冷浸的方法对刺五加花蕾中的成分进行提取，运用大孔树脂吸附色谱法、硅胶柱层析法以及葡聚糖凝胶柱层析相结合的方法进行分离，得到5种单体化合物，分别为槲皮素、槲皮素鼠李糖苷、金丝桃苷、紫丁香苷和刺五加苷E；利用高效液相色谱法对这5种化合物在刺五加花蕾中的含量进行了测定；考察了8个部位(花蕾、根、叶、果、茎干、新枝、茎韧皮部、茎皮)中这5种化合物的含量分布情况，并作一比较，为今后建立刺五加质量评价体系提供了科学依据，奠定了坚实的基础。

Tissues of the infundibular septum from 22 patients with TOF were studied and 8 patients without cardiovascular disease and collagen system disease were used as control.The collagen concentration were determined by biochemical methods,the heart function were determined by pressure curve in right ventricle.

用生化方法定量分析22例法洛四联症患者和8例非心血管及胶原系统疾病尸检者的右室心肌间质中的胶原含量和Ⅰ/Ⅲ型胶原比值，并用右室穿刺法测定反映法洛四联症患者右室收缩及舒张功能的dp/dtmax及-dp/dtmax。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。