测定法

After irradiation by~(60)Co γ-ray,the average degree of polymerization of bamboo cellulose was determined by viscosimetry method,and the crystalline structure were studied by infrared spectra and X-ray diffraction,respectively.

采用60Coγ射线对高聚合度的造纸用竹纤维素浆粕进行辐照处理，用铜氨溶液法测定辐照前后竹纤维素浆粕的平均聚合度，并采用红外光谱法及X射线衍射法研究其结晶结构的变化。

Methods:The levels of plasma ET,serum malondial dehyde and super-oxide dismutase were measured by the methods of nalysis of radioimmunoassay,thiobarbiturate and xanthin oxidasein the blood samples from 68 patients with APP,and 29 normal controls.

采用放射免疫分析法、硫代巴比妥酸法及黄嘌呤氧化酶法测定68例APP组、及29例正常对照组血浆ET、血清丙二醛及超氧化物歧化酶的含量。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

Quadratic regression orthogonal rotational combing design was used to determine the optimal enzymatic hydrolysis process. Anthrone colorimetry's method was used to measure the carbohydrates content, pH-stat method to measure the degree of hydrolysis and Kjeldahl's method to measure the recovery of protein.

采用二次回归正交旋转组合设计优化直接酶解米糠制备短肽的工艺条件，其中总糖含量测定采用蒽酮比色法，水解度(degree of hydrolysis， DH)采用pH-stat法，蛋白质回收率采用凯氏定氮法。

Methods The Mφ functions in various groups of rats with scald on the 5th and 10th day after scald were respectively determined with methods of McAb APAAP, agar bacteriolytic plate and MTT colorimetry.

应用McAb APAAP桥联酶标法、琼脂溶菌板法和MTT比色法等技术，分别测定了各组烫伤大鼠不同时相腹腔Mφ各种功能的变化。

Seven colorimetric methods Biuret, Lowry, a modified Lowry technique using bicinchoninic acid, Coomassie brilliant blue G (CBBG dye-binding in phosphoric acid, perchloric acid or hydrochloric acid, and bromophenol blue dye-binding were evaluated for determination of protein concentration in human whole saliva.

本实验以七种定量蛋白质之方法（Biuret法，Lowry法，BCA法，CBBG染料结合法、BPB染料结合法），利用三种参考蛋白质评估测定人类唾液蛋白质浓度之差异性。

The circumscription of the Minaceae, phylogenetic relationships of genera and its reative were evaluated using morphology and molecular data of rbcL and trnL-F gene of the chloroplast genome.A total of 4 genera and 19 taxa were examined in this study.And some data of Bryaceae were downloaded from Genebank for this analysis. Phylogenetic trees obtained from neighbour jioning and maximum parsimony methods.

本研究用直接测序法测定了提灯藓科4属19种植物的rbcL和trnL-F基因序列，并从Genebank中下载了该科和真藓科的部分基因序列，采用邻接法和最大简约法分别构建了rbcL和trnL-F两个基因片段的系统树，并结合形态方面的分支分析，探讨了提灯藓科的范畴、科内各属的亲缘关系及与其临近科间的关系。

Raeusch fruit. Total phenolic content and phenolic compositions of Canarium album Raeusch fruit from Fujian area were analyzed by Folin-Ciocalteu colorimetry, UV spectrum and HPLC method.

方法］采用Folin-Ciocalteu比色法、紫外吸收光谱法和HPLC色谱法分别对福建檀香橄榄果实中的酚类含量和酚类化合物组成进行了分析测定。

Impact of PCL capsulation on the mesenchymal stem cell activity studied in vivo, one plate culture with complete culture medium for control group with and two encapsulated transplantation for experimental groups(complete culture medium or basic culture medium for cell suspended) installed,after package and transplantation,retrieving capsulated cells for cell count,MTT assay,CCK-8 assay Daily for 8days to determine cell activity,and analysis growth curve.

以完全培养液平皿法细胞培养为对照组，设2个实验组进行聚己内酯囊管化骨髓间充质干细胞移植细胞活性研究。两个实验组分别以完全培养液和基础培养液悬浮细胞后封装移植，囊管内细胞回收后以细胞计数、MTT法、CCK-8法测定细胞的活性，共8天，进行生长曲线分析。

The components of fatty acid in carya cathayensis oil are obviously more than that extracted by Soxhler, and the contents of unsaturated fatty acid ...

经分析测定超声波法提取山核桃油的脂肪酸成分明显多于索氏法，其不饱和脂肪酸含量也明显高于索氏法提取的油脂的脂肪酸。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。