测定法

Saving water with the Ground Cover Rice Production System in China–acritical evaluation.

打孔称重法与拷贝称重法和长宽校正法测定水稻叶面积的方法比较。

Three analysis methods were used in this study to analyze the content of amygdalin , and the effective components in bitter apricot kernel, namely, HPLC method, UV-Spectrophotometric method and Second-derivative spectra method.

苦杏仁的有效成分苦杏仁苷经提取、处理后，用HPLC法、紫外分光光度法、二阶导数光谱法测定其含量。

Methods Based on the sugar analysis of 10 tea polysaccharides, the mix of arabinose:galactose:glucose (1:1:0.5, mol ratio), which was the major sugar composition in tea polysaccharides, were applied to investigate the influence of sugar on the carbazole method and meta-hydroxydiphenyl method respectively.

采用气相色谱法分析了10种茶多糖样品单糖组成，考察了其主要的三种单糖组分组成的混合中性糖（1：1：0.5，摩尔比）对硫酸咔唑法、间羟联苯法两种方法测定糖醛酸含量的影响。

But in stationary phase and decline phase, the latter method ould reflect the growth situation of Azotobacter NT06 authentically.

通过分光光度法和活菌计数法分别对固氮菌NT06的生长曲线进行测定，结果表明对数期以前两种方法所测结果基本一致，而在稳定期和衰亡期，活菌计数法能更真实地反映细菌的生长情况。

The concentrations of serum thyrotropin in 48 normals, 20 hatients with hyper thyroidism,10 patients with hypothyroidism and 121 inhabitants in the endemic goiter area were measured by monoclonal-antibody two-side solid-phase EIA and immunoradiometric assay.

应用单克隆抗体双面固相酶联分析法和免疫放射分析法测定48例正常人，20例甲亢病人、10例甲低病人和121例地方性甲状腺肿流行区居民的血清TSH.TSH—EIA法的结果平均值在各组中均低于IR MA法的结果。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Their direct electrooxidative behaviors and the parameters of electrochemical kinetics were obtained by cyclic voltammetry,bulk electrolysis with coulometry,chronocoulometry,chronoamperometry.

运用循环伏安法考察了苦参碱和槐定碱的直接电化学氧化行为，用控制电位电解库仑法、计时库仑法、计时电流法测定并计算了它们的电化学动力学参数。

Based on a lot of documents, this thesis chooses several kinds of organic or drug small molecular, using resonance light-scattering technique, UV/Vis absorption spectra method, fluorescence spectra method and 1H NMR spectra method studied the interaction mechanisms of those small molecular and biopolymer. Base on the variation of the intensity of resonance light scattering is ratio to the concentration of the protein and DNA establishes several new determination methods of protein and DNA.

本文在查阅大量文献的基础上，选择了几种有机或抗癌药物小分子，用共振光散射光谱法并结合吸光光度法、荧光光谱法以及核磁共振光谱法研究了他们与蛋白质或核酸的相互作用，根据共振光散射光谱信号的改变值与蛋白质和核酸的浓度成正比的特点，建立了几种测定纳克级蛋白质或核酸的新方法。

Result and conclusion: The electrochemical experimental results show that the two totally irreversible oxidation peaks in the region of 0.3-1.2 V vs SCE for both MT and SR were observed,and the electrode reactions processes were diffusion-controlled.Their electron transfer number n and electron transfer coefficient α were obtained by CV and electrolysis with coulometry.The diffusion coefficient D and rate constant k f were also calculated by CC and CA.

结果与结论：在0.30~1.2 V电位窗口内，MT和SR均产生1个不可逆氧化峰，并且它们的电极反应均受扩散步骤控制；用控制电位电解库仑法和循环伏安法得到MT和SR的电极反应电子转移数n和电荷转移系数α，运用计时库仑法和计时电流法测定了它们的扩散系数 D 及电极反应速率常数kf。

The study makes out several useful evaluational method by observing, test paper investigating and visiting to evaluate HCPE.

本研究提出了评价体育隐蔽课程常用的方法有观察评价法、问卷评价法、访谈评价法、相关系数测定评价法等。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。