测定法

The purpose of the study is to investingate the feasibility of closed-microwave method instead of traditional aqua regia hotplate reflux method for heavy metal determination in soil.

中文摘要本研究利用密闭微波法消化土壤，并以火焰式原子吸收光谱法分析镉、铜、铅、锌四种金属元素，目的在探讨以密闭微波法取代传统王水热板回流消化法，测定土中重金属之可行性。

On the basis of Kjeldahl, Coomassie Brilliant Blue ,Folin-hydroxybenzene,Biuret was used to determine the human-like collagen produced from the recombined E.

以凯氏定氮法为基准方法，采用考马斯亮蓝法、Folin-酚法、双缩脲法对从重组大肠杆菌发酵产物中提取的类人胶原蛋白含量进行了测定。

Free carboxyl group was determined by the wet chemical analysis method, acid-base titration, in this paper.

在几种测定方法—氢氧化钠直接滴定法、氢氧化钠反滴定法、醋酸钙法中，醋酸钙法较好。

It is stated systematically in the thesis the methods theories and measuring principle of all kinds of geostress determination technology. In particular the four methods recommended in 1987 by the committee of test method of the International Society of Rock Mechanics, i.e. two over-coring methods of strain relief in boreholes, hydraulic fracturing method and stress furbish measurement which measures the surface stress.

本文较为系统地、全面地介绍了当前国内外通用的各种地应力测量方法的理论、测量原理；特别就国际岩石力学学会试验方法委员会于1987年推荐的4种地应力测定方法(钻孔孔径变形测量法、钻孔孔壁应变测量法、水压致裂法以及测量岩体表面应力的应力恢复测量法)作了较为详细的叙述。

In addition, the preparation and package and store for standard reference materials of antioxidant BHT are studied.

本论文主要叙述了对工业品防老剂BHT的重结晶提纯和区域熔融提纯(自行设计和制造了区域熔融仪，申请了发明专利)来制定防老剂BHT标准物质的研究：通过实验确定了乙醇重结晶提纯工艺；利用自行设计区域熔融装置，拟定操作规程和技术条件；确定提纯样品的高效液相色谱法和差示扫描量热法的纯度检测条件和实验方法；用高效液相色谱检验BHT标准物质的均匀性、稳定性；分别用高效液相色谱法和差示扫描量热法测定这种标准物质的不确定度评定及两种不同原理检测的定值分析；最后确定标准物质BHT的纯度和合成标准不确定度：标准物质的制备、包装及贮存。

Lysis solutions from imported and domestic genomic DNA purification kits,and 2% Trition X-100 and 5%guanidinium isothiocyanate were added into the oocysts,followed by different combining forms of freeze-thawing,proteinase K and sonication.Real-time PCR was used to determine the copies of Cryptosporidium oocyst wall protein gene.HCT-8 cells were infected by C.andersoni oocysts.Real-time PCR was used to determine the enhancement effect of fetal calf serum,glucose,ascorbic acid,folic acid,calcium pantothenate and insulin while added into RPMI-1640 medium.

在隐孢子虫卵囊悬液中分别加入进口试剂盒和国产试剂盒的裂解液以及2%Triton X-100和5%异硫氰酸胍，辅以不同组合方式使用的冻融法、蛋白酶K法和声裂法裂解卵囊，通过Real-time PCR法测定裂解产物中隐孢子虫卵囊囊壁蛋白(Cryptosporidium oocyst wall protein，COWP)基因拷贝数。

Methods MSCs were obtained from tibias by density gradient centrifugation and were tested the rate of adhesion. The configuration was observed under phase-contrast microscope consecutively and the cell growth was measured by MTT method. Cell cycle and surface antigens were detected through flow cytometer.

测定其接种贴壁率，在倒置显微镜下连续观察细胞的形态变化，利用甲基噻唑基四唑（methyl thiazdyl tetrazolium， MTT）法测定 MSCs 生长曲线，并应用流式细胞术测定细胞表面标记物。

The determination of industrial zinc sulphide content by redox titration method was introduced.

利用氧化还原滴定法测定工业硫化锌的含量，分析了影响硫化锌含量测定的因素，样品测定实验结果表明，该法样本合并标准偏差s_q=0。

We killed some mice at the day 6th , the others were continue to expose to formaldehyde and observe their estrous cycle. HE staining was used to study the pathological changes happened in ovarian tissue. Uranium acetate and lead acetate double staining was used to study the ultrastructure of oocyte. E2 and LH level in blood serum was measured by radioimrnunoassay reagent kit.

第6d处死一批小鼠，用HE染色观察小鼠卵巢组织的病理改变，用醋酸铀—醋酸铅双重染色电镜下观察小鼠卵母细胞超微结构的损伤；用GSH测定试剂盒测定小鼠卵巢组织中的GSH含量；用E_2和LH放射免疫分析试剂盒分别测定小鼠血清中E_2及LH水平；采用超排卵法，计数超排卵数并收集卵母细胞，于油镜下观察生殖细胞遗传物质的损伤。

Methods:Unconfident Pk deficiency subjects from associate hospitals of Kunming Medical College.PK activity,thermostability, PEP kinetics, ATP inhibition and optimum pH were measured by ICSH recommended methods to confirm deficient in PK.The contents of Na~,K~,Ca~(2+),Mg~(2+) in erythrocyte PK deficiency were quatitated by inductively coupled plasma atomic emission spectrometry.

以昆医附院提供的PK缺陷可疑患者为研究对象，按照国际血液学标准委员会推荐的方法，对PK缺陷一个家系进行活性测定、热稳定性测试、PEP亲和力试验、ATP抑制试验及最适pH值测定，应用电感耦合等离子体原子发射光谱法测定红细胞钠、钾、钙、镁离子浓度。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。