测定法

Six rats of each group were randomly sacrificed on day 7,14,28 and 42 respectively.The histological changes of lung tissue were studied by HE and Masson's trichrome staining.Hydroxyproline level in lung tissue was measured by digestion method.Protein and mRNA expression of transforming growth factor-β1（TGF-β1） were assayed by immunohistochemistry and RT-PCR respectively.

各组分别于造模后第7、14、28及42 d随机处死6只，取肺组织行HE、Masson染色评价肺组织病理变化，消化法测定肺组织羟脯氨酸含量，分别采用RT-PCR和免疫组化法测定肺组织转化生长因子β1（TGF-β1）的mRNA和蛋白表达。

DNA extraction quality of Venturia inaequalis using CTAB method,SDS method,Parker's method and modified SDS method was detected by ultraviolet spectrometer.

采用CTAB法、SDS法、Parker法及改进的SDS法提取苹果黑星病菌的DNA，经紫外分光光度计测定，改进SDS法提取的DNA得率较其他3种方法高，约为Parker法的2倍。

Methods GR mRNA was meaanred by Northern blots and hybridizations. GR binding activity was measured by Wrang's method.

方法采用分子生物学杂交技术、Northern印迹法测定胎鼠肺及其二种主要细胞的GR mRNA及Wrang法测定外源性糖皮质激素对胎鼠肺及二种主要细胞GR结合活性的作用。

METHODS:①Sixty samples were randomly divided into two groups(test and contrast groups,n=30 respectively),and the sciatic nerves of the toad was isolated.Two sorts of solution(alkalinized and plain bupivacaine hydrochloride) were applied to a segent of nerve,and the amplitude of the action potential was measured.②The grouping method was the same as above.After destroying the cord above the third spinal vertebrae,two sorts of bupivacaine soultion in various concentration were injected into abdominal cavity of the toad.The leg′s flexing time was measured.

①离体坐骨神经法：取蟾蜍60只，制备坐骨神经标本，分两组，实验组和对照组各30只，测定不同浓度的两组布吡卡因对离体坐骨神经动作电位幅度的影响；②在体坐骨神经丛法：取蟾蜍60只，分组同上，破坏第三脊椎以上的脊髓后，将不同浓度的两种布吡卡因注入蟾蜍的腹腔内，测定其缩腿反射消失的时间。

Two sorts of solution(alkalinized and plain bupivacaine hydrochloride) were applied to a segent of nerve,and the amplitude of the action potential was measured.②The grouping method was the same as above.After destroying the cord above the third spinal vertebrae,two sorts of bupivacaine soultion in various concentration were injected into abdominal cavity of the toad.The leg′s flexing time was measured.

①离体坐骨神经法：取蟾蜍60只，制备坐骨神经标本，分两组，实验组和对照组各30只，测定不同浓度的两组布吡卡因对离体坐骨神经动作电位幅度的影响；②在体坐骨神经丛法：取蟾蜍60只，分组同上，破坏第三脊椎以上的脊髓后，将不同浓度的两种布吡卡因注入蟾蜍的腹腔内，测定其缩腿反射消失的时间。

Enzyme-linked immunosorbent assay and coagulating time method were used to assess antigenic stability and coagulating stability,respectively.

以酶联免疫吸附法测定了蛇毒降纤酶的抗原活性稳定性，并与凝固时间法测定的生物活性稳定性进行了比较。

Enzyme-linked immuno-sorbent assay and coagulating time method were used to assess antigenic stability and coagulating stability, respectively.

以酶联免疫吸附法测定了蛇毒降纤酶的抗原活性稳定性，并与凝固时间法测定的生物活性稳定性进行了比较。

Those methods can be used to determine the content of naringin, pectin and biofavonoids in the peel or the extraction in those ranges of linear line.(2) The process of extraction, discoloring, separation of pectin is studied in detail.

同时建立了样品中生物类黄酮的分光光度法测定方法和样品中果胶含量的咔唑显色法测定分析方法。

METHODS Acute incomplete ischemia was induced by ligaturing bilateral carotid arterise and cutting heads in mice, the survival time and asthmatic time were observed; the coagulation timewas measured by sheet glass method,and the blood viscosity was also assayed; the platelet aggregation induced by collagen were studied by turbdimetry in vitro.

采用结扎小鼠双侧颈总动脉造成急性不完全性脑缺血模型，观察平均存活时间；另取小鼠断颅后测定断颅喘息时间；采用玻片法，观察胡芦巴总皂苷对凝血时间的影响；采用比浊法；观察其对体外血小板聚集率的影响，并测定全血粘度。

Respectivey, The methylmercury levels of human hair for mercury mine workers, children and pregnam women living near the mine area have been compared with that of those living at nonpol luted .

本文采用气相色谱法测定人发中甲基汞，对人发的酸度、浸取时间、人发与酸液的比例，以及无机汞存在的影响作了测定和选择，方法的平均回收率96.6%，精确度2.8%，方法可靠性用氢化物原子荧光法核对，结果一致。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。