测定法

After 4 passages in culture, the MSCs were implanted into 96 foveola with cell density of 0.08,0.1,0.2,0.4,0.6,0.8,1,2,4,6,8,10(x104/ml) respectively. Meanwhile, the MSCs were cultured in Dulbecco's modified Eagle's medium culture medium with different concentration of fetal bovine serum, the 3-4,5dimethylthiazol-2-yl-2,5-diphenyl tetrazelium bromide assay were used to determine the proliferation index of MSCs 72 hours later, the cell cycle were determine by cytometer also.

贴壁筛选法分离、纯化MSCs，取4代以后细胞，以不同密度接种于96孔培养板内，分别以不同浓度血清进行培养，72h后，采用四甲基偶氮唑盐比色法测定MSCs增殖能力并用流式细胞仪测定细胞周期。

The gravimetry method by directly adding calcium carbide to determine the moisture content in tar was introduced, and was compared with the distillation method.

介绍了直接加入电石测定焦油水分的称量法，并且与蒸馏法进行了对照，用新方法测定的焦油样，其精密度和准确度较高。

According to the bone apposition events at the interface, we measured the osteoblast attachment rate with the hemocytometer, the osteoblast growth curve with modified MTT method, the ALPase activity and protein content of cellular layers with modified kinetic method and modified Coomassie'method respectively, and osteocalain content in the cell medium with RIA , at different levels of cell responses, as osteocompatibility indexs.

根据骨内种植体界面的骨愈合过程，分别从成骨细胞反应的不同层次选择了细胞贴壁率、细胞生长曲线、细胞层ALPase活性和蛋白质含量以及细胞培养液中骨钙素的含量作为体外评价材料骨性生物相容性的指标。采用细胞计数板计数法测定细胞贴壁率，采用改良MTT法测定细胞生长曲线。

Methods: The changes of surum-level T 3,T 4,FT 3,FT 4 and TSH in examinee by RIA were observed.The blood glucose were observed by methods of hexokinase.

用放射免疫法测定受试者血清 T3、T4、FT3、FT4、TSH的水平，用己糖激酶法测定血糖。

The effect of different infustion water methods to humidify coal seam were determined. The awash method and carve wall method were taken to measure the dust-adhering bar's function to prevent vaporization.

测定了不同注水方法润湿煤体的效果；分别采用浸润法和煤壁刻槽法测定了粘尘棒防蒸发性能；进行了普通注水与添加粘尘棒注水降尘的对比试验。

Materials and Methods : Serum samples were collected from out-patients and in-patients with confirmed hysteromyoma in our hospital and stored at freezing temperature (- 20℃) to be tested; the change of SOD activity was detected with hydroxylamine method and the serum MDA level was measured with TBA method; in the meantime, 20 health women were taken as the control group.

材料与方法：收集本院门诊和住院病人经病理确诊为子宫肌瘤患者的血清，-20℃冰冻保存待测，分别采用羟胺法测定血清超氧化物歧化酶活性的变化，采用TBA法测定血清丙二醛的水平，同时以23例健康人作为对照。

MethodsSaccharide content was determined by anthracene ethyl ketone method;amino acid content was estimated by indene tri-ketone colorimetric method.

方法青蒿可溶性糖含量测定使用蒽酮醋酸乙酯法，青蒿游离氨基酸含量测定用茚三酮比色法。

A noncomplete digestion-flame atomic absorption spectromery for rapid determination of iron and zinc in fruits have been developed and the level curve method was used to determine.

建立了非完全消化-火焰原子吸收光谱法快速测定水果中的铁、锌的分析方法，用标准曲线法测定。

The effects of Cortex phellodendri alkaloid on splenic lymphocyte proliferation after heat stress were detected by MTT chromatometry.On the basis of the above detection,CPAs extracted by limewater,ethanol,sulfuric acid water and the mixture of sulfuric acid-and-ethanol were choosen.

采用MTT法测定了热应激后小鼠脾淋巴细胞增殖的情况，以此作为醇提、碱提、酸醇提、酸提4种提取方法所得黄柏生物碱的筛选依据；采用ELISA法测定了醇提黄柏生物碱对热应激后小鼠脾淋巴细胞IFN-γ、IL-4分泌水平的动态影响。

The mouse splenocytes were induced with Rg3, and the activity of IL-2 was detected by lymphoblast proliferation assay and IFN-γ by cytopathic effect inhibition.

以Rg3诱导小鼠脾细胞，用鼠脾T淋巴母细胞增殖分析法测定上清中IL-2活性，细胞病变抑制法测定IFN-γ活性。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。