测定法

Local lesion assay indicated that when mixed with TMV-infected leaf juice dilution within 3 minutes, 5 disinfectants showed 100% inactivation ratio, including 30% chloride of lime 1:9 solution, 98% trisodium phosphate 1:9 solution, 7% sodium hypochlorite 1:150 solution, anonymity disinfectant used in USA 1:125 solution, and 75% alcohol solution.

摘 要：为了指导烟草漂浮育苗中剪叶工具消毒剂的使用，采用半叶法测定了9种药剂对烟草病叶汁液中TMV的钝化效果，采用叶面喷雾法测定了药剂对烟草的药害浓度与药害症状。

Methods: The total polysaccharide was extracted with distilled water from mycelia of paecilomyces cicadae. The colorimetric MTT was used to analyze the proliferative activity of PBMC, U937 and K562 which were treated with PCPS of 7 concentrations respectively in vitro. ELISA was used to detect the content of hTNF-α, hIFN-γ from supernatant of culturing PBMC that was treated with PCPS for 44 h.

用水提取蝉拟青霉菌丝体总多糖，以MTT法测定不同浓度总多糖对人外周血单个核细胞及白血病细胞株U937、K562增殖的影响，ELISA法测定多糖作用44h后，外周血单个核细胞培养上清液中hTNF-α、hIFN-γ的含量。

The contents of total flavones in Marchantia convolute were determined through developed visible spectrophotometry. The reversed phase high performance liquid chromatography was set up to determine the contents of flavonoids in Marchantia convolute.

采用改进的铝盐显色分光光度法测定拳卷地钱总黄酮含量；采用反相高效液相色谱法测定拳卷地钱黄酮类化合物中芹菜素、槲皮素和木犀草素的含量。

The method, which is capable of effecting very good separation of phenol and the 3 cresol isomers under the same chromatographic conditions, can be used to determine phenolic compounds in phenolic tar by external standard, and to analyze these compounds in the toxic leaching solution of the tar.

摘 要：建立了酚焦油中酚类物质质量分数测定和浸取毒性测定的气相色谱法，此法可以在同一色谱条件下对酚焦油中的苯酚及邻、间、对甲酚实现很好的分离，利用外标法分别进行定量分析，并对酚焦油浸取液中相应组份进行测量。

Abstracts The average molar weight of Sulfated polysaccharide 911 was determined by the content of its sulfate group, which was determined by the oxygen flask method at first in this paper, then the content of Sulfated polysaccharide 911 was determined by the decarboxylation method.

摘 要 该文采用氧瓶燃烧法测定了硫酸多糖911的硫含量，根据所测硫含量确定了硫酸多糖911的平均单体式量，然后在此基础上应用脱羧法对硫酸多糖911样品的含量进行了测定。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型，连续灌胃6周，处死动物取血，检测血清ALT、AST、ALB；ELISA法测定血清透明质酸；取肝、睥，并计算肝、脾指数，组织切片HE染色后进行组织病理学疗效评估；用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达；半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

After hybridization of two DNA probes with target DNA, EDTA-〓 and β-diketone come close to each other, and an EDTA-〓-β-diketonate ternary complex having strong and long-lived fluorescence was formed, thus the target DNA was detected sensitively with a detection limit of 6 pM (0.6 fmol per assay) by time-resolved mode.

系统研究了使用EDTA-〓为标记物的石墨炉原子吸收光谱免疫分析法，并将之用于人血清中AFP和水中BSM的测定。该法中使用了EDTA-〓标记SA及生物素标记抗体或抗原，在96孔板中的免疫反应结束后，利用稀硝酸将反应产物分解，然后用石墨炉原子吸收光谱法对免疫反应产物进行测定。

Meanwhile the eluate was determined by high performance liquid chromatography, the results from HPLC correlated well with those from ELISA.

同时采用高效液相色谱法测定洗脱液，与ELISA法测定结果基本一致。

Chinese chestnuts of Qianxi were dealed with by wet method and the determination of the content of Se in chestnuts was discussed on the influence of the proportion of nitric acid and the perchloric acid. In the system of ferricyanide potassium and hydrochloric acid, the determination conditions were optimized by standard-curve method and quantitative analysis was acquired.

采用湿法处理河北迁西板栗样品，探讨了硝酸与高氯酸的配比对板栗中硒元素含量测定的影响，在铁氰化钾－盐酸体系中，对测定条件进行了优化，采用标准曲线法定量分析。

The paper analyzes the shortage of traditional roadway measuring wind method,and by theoritical calculation of measurement,the wind amount is tested,and the roadway fixed central point method of wind measurement and the roadway s average wind velocity fixed-point method of wind measurement are proposed,and which solve the problem of low wind velocity and low credibility well.

分析传统井巷测风方法的不足，并通过风量测定的理论计算和对现场风量进行实际验证，提出了井巷中心点定点测风法和井巷平均风速点定点测风法，较好地解决了当前测定风速慢、可靠性差的问题。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。