测定法

Chemical methods of qualitative assay and quantitative assay to Chufa functional component According to chemical characteristics reactivity of Chufa functional component, chemical method of qualitative assay and quantitative assay to Chufa functional component were built.

功能因子的定性定量化学分析针对该功能因子的化学特征反应，实验选用硫酸显色法、可见光比色法、吸光度测定法和沉淀法对功能因子进行定性、定量分析。

Determination by volunteers depends on subjective description, taste of no grittiness representing complete disintegration. Moreover, modification of pharmacopoeial disintegration testing methods depends on disintegration without shake, the suspension passing through 10 sieve representing complete disintegration.

志愿者口服试验法以服药后的主观感觉为依据，无粗糙砂砾感即为崩解完全；而崩解度检查方法改进法采用不加振摇的崩解度测定法，片剂全部通过10目筛即为崩解完全。

Pararosaniline was the most accurate and sensitive method of determining SO2 residue, and it was fit for the samples of any SO2 content. Improvement pararosaniline method was only fit for the samples of high SO2 residue. Iodometry was not fit for the deep color samples and low SO2 residue samples. The fast determination method could be used the quality and semi-quantitative determination of SO2 residue in edible fungus.

副玫瑰苯胺比色法是检测SO2残留最准确、最灵敏的方法，适合于各种SO2浓度的样品；改良的副玫瑰苯胺比色法只适合于高SO2残留的样品；碘量法不适宜检测深颜色的食用菌，而且在检测低SO2残留样品时准确度不高；快速测定法可用于食用菌产品中SO2残留的定性检测和半定量分析。

Several techniques are discussed: scintigraphy, paracetamol absorption test, breath tests, refractometry, ultrasound, and gastric impedance monitoring.

我们讨论了以下这些技术：闪烁显像法、对乙酰氨基酚吸收试验、呼吸试验、折光测定法、超声法和胃阻抗监测法。

The article analyzes change of its ingredients including Ca(superscript 2+), Fe(superscript 2+) 42(superscript 2+) within different process using chemical analysis, iodine amount and testing chemical value ,and prove the conclusion that the method abouve is one of the best ways to process ruddle through experimental data, which may cause ruddle crispy and easy to be smashed, and get the effctive ingredients, and arsenic's amount can be limited as the value require by codex (0.01%).

利用化学分析法、碘量法以及化合价数的测定法来分析代赭石不同炮制品中的Ca(上标 2+)、Fe(上标 2+)、As(上标 3+)的含量变化，通过实验数据来证实煅醋淬法是炮制代赭石最行之有效的一种理想方法，会使代赭石质地最酥脆，易于粉碎，使有效成份最易煎出，最能使炮制品中冲的含量(0.01%)符合药典要求，建议把砷的含量作为股代赭石的一个质量指标。

1H NMR spectroscopy, viscometry, and differential scanning calorimetry were employed to characterize this novel type of crown ether-based supramolecular network

我们用氢核磁共振光谱法、粘度测定法和差示扫描量热法来表征这一类新的冠醚基超分子网络。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

In this paper, in accordance with the mixed method, a measuring system, which consisted of a hydrothermograph with magnetic force agitator, a potential difference meter and a digit thermoammeter etc., were used in the determination of heat capacity of short paddy grain growing in the south China, with a special attention to the influence of moisture content upon the heat capacity of rough rice.

该研究根据混合测定法原理，用磁力搅拌式水卡计、电位计以及数字温度计等，组成一套测定装置，对我国南方产短粒型稻谷的热容进行测定，着重研究了稻谷的含水率对热容的影响。

Objectives:To introduce the author's experiences in bioassay of platelet activating factor with thin layer chromatographic separation and platelet aggregation method,and to discuss probable problems in the process.

目的：介绍作者应用薄层层析分离和生物测定法测定血小板激活因子的体会，重点讨论分离和测定过程中可能出现的问题及注意事项。

To observe the effects of HASF on viability and collagen synthesis of two cell lines, AgNORs staining, 3H-proline incorporation and HYP determination were carried out.

层析法从兔羊水及胎兔血清中提取HASF并做活性鉴定；用嗜银蛋白染色法、3H-脯氨酸掺入法和羟脯氨酸测定法观察不同浓度的HASF对细胞增殖活性和胶原蛋白合成的影响。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。