测定法

Each group was 10 Lycium barbarum seedlings.The contents of soluble sugar,soluble protein and malondialdehyde were determined by anthrone method,Foiln-hydroxybenzene method,thiobarbituric acid method respectively.

分别采用蒽酮法、Folin-酚法、硫代巴比妥酸法测定可溶性糖、可溶性蛋白质和丙二醛MDA的含量，用电导仪测定幼苗的电解质渗透率。

Methods The molluscicidal efficacy of different concentration of endophyte JJ18 was detected according to the immersion test method recommended by WHO.The glycogen and protein of Oncomelania hupensis treated by endophyte JJ18 was determined by Anthrone and Kjeldahl method,respectively.

按照世界卫生组织的杀螺剂浸泡试验法观察不同浓度下JJ18发酵液提取物的灭螺效果，采用蒽酮显色法测定处理后钉螺软体的糖原含量变化，凯氏定氮法测定其蛋白质含量。

The application of ultrasonic method in bored concrete pile detection;2. Suo′s method and ultrasonic method for extracting BPBenzo(aPyrene content from coke oven gas were introduced.

阐述了对焦炉煤气中苯并芘含量提取处理方法中的索氏法和超声法的两种方法，分析了两种方法测定相同含浓度苯并芘含量样品测试数据差异及产生原因，用实验数据分析说明了使用超声法作为测定焦炉煤气中苯并芘含量是一种最佳的处理方法。

In this paper migration theory and techniques related to the high-energy TEGN gun propellant are presented, and some methods determining the migration of RDX are analyzed and compared. As a result, chemical and thermal analyses are employed associatively to test the content of RDX in different position of TEGN gun propellant stored different period. The antimigration capability of TEGN gun propellant making use of two different granularity of RDX is also been studied.

本文以高能太根发射药及其相关技术为研究对象，分析研究了迁移理论以及目前国内外测定RDX迁移性能的方法，对这些方法的优缺点进行了比较，并采用化学分析法、热失重法、差式扫描量热法相结合的方法对各种不同储存时期高能太根发射药不同部位中RDX含量进行了测定，研究了使用两种不同粒度RDX制备的太根发射药的抗迁移性能。

Cases healthy subjects served as control. In addition, density of glomerular matrix membrane and quantity of cell in glomeruli were measured with CMIAS image analysis system by computer. Global sclerosis, cellular crescents, fiber or fibrocellular crescents were measured with Memphis scope. Tubulointerstitial lesions were examined with semi-quantitative grades (including O-III grades).Results Compared to normal control, there was a significant increase of urinary TGF-betaK IL-6 and Col-lV levels in patients with IgAN (p. 01). There were also significantly positive correlation between the levels of urinary TGF-betaK Col-IV and density of glomerular matrix membrane ., interstitial fibrosisp.

采用酶联免疫吸附法测定尿TGF-β1(Transforming growth factor-β1)、尿IL-6(interleukin-6)和尿Col-Ⅳ(type Ⅳ Collagen)；采用免疫组织化学法检测肾组织TGF-β1、Col-Ⅳ表达；应用CMIAS多功能真彩色病理图像分析系统对肾小球基质基底膜面密度和细胞个数进行半定量测定；对球性硬化、节段硬化、细胞新月体及纤维或纤维细胞新月体所占肾小球百分数和肾小球IgA沉积免疫荧光强弱的判断采用Memphis法进行评分；肾小管间质病变程度采用光镜下半定量分级法(包括0-Ⅲ级)。

The research results indicated that the pretreatment of corn stalk by steam blasting procedure or warm oxygen method could successfully separate lignose and fibre, and enzyme hydrolysis could realize 85 % cellulose conversion rate, then yeast used for fermentation to produce alcohol.

将测定谷物色氨酸的&酶水解三氯化铁醋酸比色法&用于山羊奶、牛奶色氨酸测定，回收率可达96.4％以上。试验结果表明，该法精密度与DAB比色法无显著性差异，但比DAB比色法更快，更简便可靠

The discussions which the study investigated were chicken and pork meat processing for elders via different tender mode. In this study, we want to evaluate the nutritions of elders and focus on animal protein. The experiment was injected marination with CaCl2, NaHCO3 and enzyme on pork and chicken meat quality traits. Treatment on the meat was analyzed proximent quality and textural properties by response surface stepwise regression. The best tender method was discovered the elder's sensory evaluation of different tender mode and observed degradation of myoglobulin by muscle tissue slice and SDS-PAGE (10%). The function of sous-vide cooking collocated with tenderization that was evaluated by textural properties and sensory evaluation. It looks for discussion about meat processing for elders.

中文摘要本试验目的在利用市售禽畜肉为原料，针对高龄者之营养需求，分别使用氯化钙、碳酸氢钠与嫩化酵素等常见之嫩化方式对禽畜肉进行嫩化处理，搭配反应曲面法分析一般特性与物性测定，并进行高龄者品评测试以探讨不同嫩化方式之口感影响以求得不同禽畜肉之最佳嫩化处理，并以肌纤维组织切片与电泳分析观察肌肉蛋白质之分解现象，最后利用真空调理法搭配嫩化处理并分析物性测定与品评测试，评估真空调理法增进嫩化之效果，探讨禽畜肉开发为适合高龄者之加工产物模式。

Three common lipase activity determination methods including alkaly titrition method,ρ-nitrophenol method and copper soap method, aiming at consuming time, accuracy and repeatability, are compared.

比较了碱滴定法、对硝基苯酚法和铜皂法等三种常用的脂肪酶活力测定方法的测定时间、准确性和重复性。

Taking Phasianus colchicuses and Gallus gallus as comparisons,the contents of protein and amino acides in egg albumen, mineral in egg shell were measured by kjeldahl method,HPLC and atomic absorption method respectively.

以红腹锦鸡为研究对象，以七彩山鸡和家鸡为参照，采用凯氏定氮法和高效液相色谱法分别测定了3种鸟类卵清中蛋白质含量及17种氨基酸含量，以原子吸收光谱法测定了其卵壳中7种矿质元素的含量。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。