测定法

Nighty rats were randomly divided into the groups of control, myocardial infarction and doxycycline, which were given orally 48 hours before and 48 hours after MI with 30mg/Kg per day. Protein and mRNA extraction was done on left ventricular samples containing scar and myocardium together. Samples were assayed from 1 day to 4 weeks post-Mi. The activity of MMPs was measured by zymography and collagen amount by the method of chloramine T, the ratio of II III collagen was assessed by immunohistochemical stain. Protein and mRNA of MMP-2, 9, TIMP-1 were determined by Western-Blot and RT-PCR.

分为对照组、心梗组和治疗组(D组，术前两天至术后两天，口服Doxycycline，30mg／kg／天)，分别于术后第一天、术后一周、术后两周、术后四周取心肌组织，采用免疫组化测定胶原含量和Ⅰ／Ⅲ胶原比例，酶谱法测定心梗后MMP-2，9活性蛋白的表达规律，Western Blotting进一步确定酶谱法中所消化条带蛋白的属性，逆转录-聚合链反应法测定心梗后MMP-2，9和TIMP-1的mRNA的变化规律，运用Acuson Sequoia 512超声心动图机分别测定心梗组、治疗组在两周和四周时左室前壁、后壁厚度以及左心室舒张末内径和左室射血分数。

The content of cordyceps acid was determined with the sodium periodate method.

方法用微波法提取人工蝉花和野生蝉花的活性成分，用高效液相色谱法测定虫草素和腺苷含量，用高碘酸钠法测定虫草酸含量，用苯酚-硫酸法测定虫草多糖含量。

Methods SH-SY5Y cells, a human neuroblastoma cell line, were incubated with different concentrations of fluoride for 48 hr and somle of them were treated with vitamin E precedently. The functional situation of cells was measured by MTT method; lipid peroxidation was detected by High-Performance Liquid Chromatography; phospholipid was separated by a Silica SepPak cartridge and neutral lipids by HPLC.

体外培养SH-SY5Y人脑神经母细胞瘤细胞，在培养液中加入不同浓度的氟化物或加入抗氧化剂，培养48h后用测定细胞MTT的方法来了解细胞的损伤程度，用高效液相色谱法分离和测定培养液中脂质过氧化物水平，用过柱和比色法测定细胞生物膜磷脂含量，用高效液相色谱法测定细胞生物膜辅酶Q和胆固醇含量。

By synthetically analysizing the physical and chemical properties of all components and particle size, the content of F2641 in the JOB-iC is determined by gravimetric analysis after F2641 is seperated from HMX,TATB and PNP through ? alkali reflux. Determination conditions is set as follows the sample is boilingly refluxed for six hour in a constant temperature bath after adding lOOm! 8.0 ?? 0.1% NaOH. The solvent DMF saturated by TATB is used for extracting HMX,F21 and PNP from the sample and TATB is seperated by crucible filter G4. The mass precent of TATB is determined by extraction fractionation. The mass percent of PNP is measured by multiwavlength linear regression ultraviolet spectrophotometry. The testing conditions are set as follows:multiwavlength constitution: X=267nxn, 275nm, 283nm, 29mm, 299nm,the application scope of the Lambert-Beer law: the concentration of PNP is O.005?0.O25mgIml, the concentration of HMX is 0.060.3Omg/ml,absorption coefficents of PNP and HMX are solved by the slope of linear regression curve of absorbency- concentration of standard solution of PNP and HMIX for measuring wavelengthes,on the basis of the Lanibert-Beer law and absorbancy additivity principle, the slope of linear regression curve of A/E(1) and E1 of PNP and HMX solution is regarded as the concentration of PNP in the solution.

根据传爆药中各组分的物理、化学性质及主体炸药的粒度大小，进行综合分析，确定了采用碱回流重量法测定JOB-1C 中F_(2641)的含量，测定条件：加入100ml浓度为8.0±0.1％的氢氧化钠，在恒温水浴中煮沸回流6h；采用溶剂萃取法测定JOB-1C中TATB的含量，选择TATB饱和的二甲基甲酰胺为萃取溶剂，用G4坩埚式过虑器进行萃取分离；采用多波长线性回归紫外分光光度法测定JOB-1C中PNP的含量，通过实验确定了多波长组合：λ=267nm，275nm，283nm，291nm，299nm；朗波—比耳定律的适用范围为PNP浓度：0.005～0.025mg/ml，HMX浓度：0.06～0.30mg/ml；在测定波长下，对PNP、HMX标准溶液的吸光度—浓度进行线性回归，由回归曲线的斜率得出PNP、HMX的吸收系数；根据朗波—比耳定律和吸光度加和性原理，在测定波长下，对PNP、HMX 两组分混合溶液A_i/E_HMX(i与E_PNP(i/E_HMX(i进行线性回归，回归曲线的斜率即为混合溶液中PNP的浓度。

The level of PGE was assayed by spectrophotometer; Meanwhile, 3H-TdR incorporation method was used to detect ConA- and LPS- induced splenocytes proliferation and the production of interleukin-1 and IL-2. The content of antibodies to CII was determined by ELISA method. Pathological examination of articulus tissue was observed by Hematoxylin-eosin stain.

检测小鼠足爪肿胀度并对炎症进行评分；测定胸腺指数和脾脏指数；~3H-TdR参入法检测胸腺、脾淋巴细胞增殖反应；~3H-TdR参入法测定白细胞介素-1(interleukin-1，IL-1)和IL-2水平；ELISA法测定小鼠血清中抗CⅡ抗体含量；分光光度法检测足爪局部产生的前列腺素水平；HE染色法对关节组织作病理检查。

METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

A modified analysis method based on the determination of ozone—indigo disulphonate spectrophotometer(GB/T 15437-1995) in gas phase has been developed to detect the concentration of ozone in liquid phase. An analytical condition of λmax=612～615 nm,ε=20 000 L/, pH 2 is obtained by the titration of indigo disulphonate. Compared with potassium iodide method,it shows a higher precision and accuracy,CV＜3.0%. The results also prove that blank experiment will bring about 5.0%systematic error.

在气相臭氧浓度的靛蓝二磺酸钠分光光度法(GB/T15437-1995)基础上提出了液相臭氧浓度的测定方法，采用靛蓝二磺酸钠标准曲线法代替采用易分解的臭氧标准曲线法，得出测定条件λmax=612～615 nm、ε=20 000 L/、pH为2，通过碘量法比较实验，说明新的测定方法具有较高的精度和准确度，相对标准偏差小于3.0%；比较结果表明忽略空白实验将引入5.0%的系统误差。

It is a organic hexahydric weak acid. In different acidity, the dissociation degree and the color of reagent solvent were different also. Determined and draw the absorption curve of reagent in varies acidity, and figured out the dissociation constant by Perisic-Janjic method; the complex ratio of the reagent and bismuth was determinated by mole ratio method and continuous variation method; The apparent stability constant of the complex was calculated, the mechanism of dissociation and coordination were discussed.

2，6—二溴4—硝基偶氮氟胂是本实验室合成的测定铋的一个较好的试剂，对其进行了进一步的研究，在用柱层析法对其进行了提纯后，测定了试剂在不同酸华东师范大学硕：l：学位论文摘要度下的吸光度和最大吸收波长，绘制了其吸收曲线，用Perisic一Janjic法计算了试剂的六级离解常数；用摩尔比法和连续变化法测定了试剂与秘形成的配合物的络合比：计算了配合物的表观稳定常数，研究了其离解和配合机理，为筛选高效有机显色剂提供了理论依据。

The method uses first phase law to determine 63 DVT patient contrasts normally with 30 the F Ⅻ C of the group, use law of thing of the heart that deliver quality to detect at the same time zymogen of serous fine dissolve is active , law of enzymatic couplet immunity measures sex of antigen of content of activation of zymogen of methodize fine dissolve (depressor of content of activation of zymogen of dissolve of T-PAAg), fine - 1 antigen sex (PAI-1Ag) and immunity compare chaotic law to determine serous D-2 gets together body content.

方法采用一期法测定63例DVT患者和30例正常对照组的FⅫC，同时采用发色底物法检测血浆纤溶酶原活性，酶联免疫法测定组织型纤溶酶原激活物抗原性、纤溶酶原激活物抑制剂-1抗原性(PAI-1Ag)及免疫比浊法测定血浆D-二聚体含量。

This thesis studies the analytical methods of determination of Leucogen and Hydroxymethylnicotinamide by Ultraviolet and Atomic Absorption Spectrscopy.

本文研究了分光光度法和原子吸收光谱法测定利血生及羟甲基烟酰胺的分析方法，提出了利血生的三种测定方法和羟甲基烟酰胺的一阶导数峰-零法的测定新方法，并用于实际样品的测定，获得满意结果。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。