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It is that:(1) the related compounds in Metronidazole Buccal Tablets prepared by Chongqing Medical College can be tested by determining (321nm) the total peak area of the impurities;(2) The related compounds in Metronidazole Disodium Phosphate for Injection prepared by Chongqing Medical College can be test by determining (254nm) the total peak area of the impurities and the peak area of the largest impurity;(3) Ceftriaxone polymer in Ceftriaxone Sodium for Injection prepared by Chongqing Keru Pharmacy Co.

结果如下:①用HPLC一UV法,在321nm处检测,用归一化法计算,控制各杂质峰面积的和,可检查重庆医科大学的注射用甲硝吟磷酸二钠中的有关物质:②用HPLC一UV法,在254nm处检测,控制杂质峰面积的和及控制最大杂质峰面积,可对重庆科瑞制药股份有限公司的注射用头饱曲松钠中的有关物质实施控制;③用HPLC一Uv法,在254nm处检测,按外标法计算,可检查注射用头抱曲松钠中的聚合物;④用紫外分光光度法,在277nm波长处测定吸收度,按C6H9N303的吸收系数为377计算每片标示百分含量,可测定重庆医科大学的甲硝哇口颊片的含量均匀度;⑤用紫外分光光度法,在277run波长处分别测定释放溶液20min、50min和90min的吸收度,按甲硝哇(C6H剥303)的吸收系数为377计算释放度,可检查重庆医科大学的甲硝哇口颊片的释放度。

Determine the content of the moisture with the dry law of the atmosphere; With is it draw law draw fat , analyse the backbone fat first esterification with the gaseous phase chromatogram to ask for; Determine the content of starch by fastenning all law; Propose the alcohol and sink the law and draw many candies with water, and determine many content of candy more than the color law with the phenol - the sulphuric acid.

用常压干燥法测定水分含量;用索氏提取法提取脂肪,将脂肪甲酯化,用气相色谱分析主要成分;用旋光法测定淀粉含量;用水提醇沉法提取多糖,并用苯酚-硫酸比色法测定多糖含量。

Compared with the results of infrared polarimetry measurement,common polarimetry measurement and Ion exchange chromatography measurement,we can preliminary found that the accuracy of Near Infrared polarimetry measurement is better than common polarimetry measurement.

就糖厂中间制品的蔗糖分用近红外旋光法和普通旋光法进行了测定,并且引入离子色谱法测定作为评判,对比分析,初步得到近红外旋光法较之普通旋光法的测定准确度要高的结论。

We detected fasting plasma glucose by glucose oxidase,fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology,tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index,that is,ISI=1/FPG×FIns.

血糖测定采用葡萄糖氧化酶法;胰岛素测定采用化学发光法;血脂测定采用酶法;肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法;胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数,即ISI=1/FPG ×FIns。

We detected fasting plasma glucose by glucose oxidase, fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology, tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index, that is, ISI=1/FPG×Fins.

血糖测定采用葡萄糖氧化酶法;胰岛素测定采用化学发光法;血脂(甘油三酯TG、高密度脂蛋白HDL-C)测定采用酶法;肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法;胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数,即ISI=1/FPG ×FIns。

S. bumalda from the Xiaochang County in Hubei Province was sunned to 90% dryness and dried to constant weight in the oven at 105 ℃, then grinded and its protein content was detected by micro-kjeldahl method, the soluble sugar content was detected by anthrone colorimetry, the crude fat content was detected by soxhlet extraction method, total ash content was detected by the method of burning with high temperature.

方法]将晒至9成干的湖北孝昌县珍珠花于105℃的烘箱中干燥至恒重,然后粉碎,凯氏定氮法测定其蛋白质含量,蒽酮比色法测定其可溶性糖含量,索氏抽提取法测定其粗脂肪含量,高温灼烧法测定其总灰分含量。

METHODS AK was purified by DEAE ion-exchange cellulose(DEAE-52) and sepharose Cl-4B .The component of the saccharide was determined by GC and TLC.Its MW was measure d by gel filtration.The form of glucosidic bond was detrermined by IR.The conten t of the saccharide was measured by colorimetry.

采用DEAE纤维素(DEAE-52)柱层析和凝胶柱层析分离纯化草乌多糖,气相色谱法和薄层层析法测定其多糖组成,凝胶过滤法测定其相对分子质量,红外光谱测定其苷键类型,比色法测定其糖含量。

After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.

实验评估:手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量;应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平;以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜;以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。

In addition, a pre-column derivatization HPLC method was applied to the determination of Astragaloside Ⅳ; the flavonoids was determined by full-range HPLC scanning; a colorimetric method was developed for the determination of flavonoids and saponins. Hierarchical cluster analysis was performed on three constituents of different Radix Astragali species, and its result was compared with those of traditional phytoecological identification and the pharmacological quality assessment.

本研究以比色法测定了黄芪中总皂苷的含量,以柱前衍生化高效液相色谱法测定了黄芪中黄芪甲苷的含量;以比色法测定了黄芪中总黄酮成分的含量,以反相高效液相色谱法测定了黄芪中毛蕊异黄酮和毛蕊异黄酮苷的含量;以黄芪中的黄芪甲苷、毛蕊异黄酮和毛蕊异黄酮苷为主要特征对不同品种的黄芪进行了系统聚类分析,并将聚类结果与传统生药学鉴定和药理质量评价结果进行了比较。

Using dual energy X-ray bone density instrument XR-36 model to detect the bone mineral density of the left femur of the rats. Using the strain test system, we examined the biomechanics index according the tension and three-spot bending test; we also examined the bone calcium of the lumbar vertebra through plasma emission spectroscopy . We examined the serum calcium and magnesium by using the nomal biomechanics method,and examined the serum acid phosphatase and osteocalcin by using the Elisa method.

使用NORLAND公司XR-36型双能X线骨密度仪,采用小动物骨密度测定软件,测定大鼠左后肢股骨的骨密度;使用应变测试系统,按股骨拉伸和三点弯曲试验,测定股骨生物力学指标;以等离子体发射光谱法测定腰椎骨骨钙含量;以常规生化法测定血清钙、镁含量及血清酸性磷酸酶含量;以Elisa法测定骨钙素含量。

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