测定法

Objective: To establish the determination method of amygdalin in bitter almond by HPLC.

目的：建立苦杏仁中苦杏仁苷的HPLC测定方法；方法：通过单因素考察确定样品处理方法，建立 HPLC 测定方法并进行方法学考察，与药典法进行比较试验；结果：用HPLC法测定苦杏仁中苦杏仁苷含量高于药典的滴定法，方法学考察各项指标均符合要求；结论：用HPLC法测定苦杏仁中苦杏仁苷含量灵敏度高、重复性好，样品处理方法简便，可代替滴定法。

The basic principle, advantage, disadvantage and the improvement for measuring liquid surface tension with different methods were described. The methods were capillary rise method, Wilhelmy plate method, pendent drop method, drop volume method, maximum bubble pressure method. The problems in the surface tension measurement of wide temperature and pressure range, the chosen of experimental methods and the development tendency of these were introduced especially in this paper.

本文着重介绍了几种液体表面张力的测定方法（包括毛细管上升法、Wilhelmy盘法、悬滴法、滴体积法、最大气泡压力法），包括这几种方法的测定原理、优缺点及改进方法，特别指出了宽温度和压力范围的表面张力测定存在的问题，实验方法的选择及表面张力测定方法的发展趋势。

The basic principle,advantage,disadvantage and the improvement for measuring liquid surface tension with different methods were described.The methods were capillary rise method,Wilhelmy plate method,pendent drop method,drop volume method,maximum bubble pressure method.The problems in the surface tension measurement of wide temperature and pressure range,the chos...

本文着重介绍了几种液体表面张力的测定方法(包括毛细管上升法、Wilhelmy盘法、悬滴法、滴体积法、最大气泡压力法)，包括这几种方法的测定原理、优缺点及改进方法，特别指出了宽温度和压力范围的表面张力测定存在的问题，实验方法的选择及表面张力测定方法的发展趋势。

Methods The learning and memory capability of mice was investigated by Y-water maze test and step-down test. The contents of protein, DNA and RNA were investigated by Lowry methods, the diphenylamine reagent method and Oricinol reagent method.

用Y-型水迷宫法、跳台法测定小鼠的学习记忆能力，Lowry法测定小鼠脑内蛋白质，二苯胺试剂显色法测定DNA，Oricinol试剂显色法测定RNA的含量。

Chromic trioxide and chromium hemitrioxide are determined with iodometry, the coefficient of variation of chromic trioxide is 0.056%, and the recovery of marked compound is 99.82-99.83%; the coefficient of variation of chromium hemitrioxide is 1.45%,and the recovery of marked compound is 98.55-99.26%; iron is determined by complexometric titration, the coefficient of variation of iron is 1.73%, and the recovery of marked compound is 99.12-99.23%;sulfate is determined by complexometric titration, the coefficient of variation of sulphate is 0.16%, and the recovery of marked compound is

Analysis Of The Chromium Electroplating Wastewater By Oxidation-reduction And Complexometric Titration-化学工程与工艺-本文采用氧化还原滴定法与络合滴定法对镀六价铬的废水中铬酸酐，三氧化二铬，铁和硫酸根的含量进行了测定：铬酸酐和三氧化二铬用碘量法测定，铬酸酐的变异系数为0.056%，加标回收率为99.82-99.83%，三氧化二铬的变异系数为1.45%，加标回收率为98.55-99.26%；铁用络合滴定法测定，其变异系数为1.73%，加标回收率为99.12-99.23%；硫酸根用络合返滴定法测定，其变异系数为0.16%，加标回收率为

Four testing methods to detect the pollen viability of cucumber were being compared. The results indicated that I-KI staining method and acetic red dyeing were not suitable to judge the pollen viability of cucumber. Tetrazolium method can be used but the error is oversize. By and large, the Germination in vitro method was an efficient, stable and simple way to judge the pollen viability of cucumber. The component of culture medium is 10% sucrose+ 100mg/L H_3BO_3. If inoculated intraday, collect pollen of fresh abloom flower at 6:00 and if inoculated at the second day, collect before dark.

比较了4种常用的花粉活力测定方法——I-KI染色法、醋酸洋红染色法、TTC染色法和离体萌发法，认为I-KI染色法和醋酸洋红染色法不适于测定黄瓜花粉生活力，TTC染色法虽适用但误差较大，综合考虑各项因素，确定离体萌发法是最准确、简便、稳定的测定黄瓜花粉活力的方法。

After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响，ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量，检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

He sample is dissolved in a mixture of nitric acid and potassium chlorate.The determinations of Te,Bi,Fe and Cu are performed on a flame atomic absorption spectrophotometer.The selenium is determined photormetrically with 3,3'-diaminobenzidine,and the sulfur is determined gravimetrically as barium sulfate,precipitated frorm an EDTA-citric acid medium.

辉碲铋矿物经HNO＿3－KCIO＿3溶解，用火焰原子吸收法或乙二胺四乙酸二钠络合滴定法测定Bi，火焰原子吸收法测定Te、Fe、Cu，3，3＇二氨基联苯胺光度法测定Se，硫酸钡重量法测定S。

Analysis on main points for determination of chlorine dioxide by iodometric method ;2. The experiment results show: To measure sulfide in wastewater by iodometric method may have errors because the interferent can not be removed.

用碘量法测定废水中的硫化物，可能因无法消除干扰而使测定结果存在较大误差；对于同一废水样品，当碘量法和亚甲基蓝分光光度法的测定结果存在显著性差异时，可用Pb2半定量法进行判定；对于某些化纤企业的废水，用氢氧化钾-乙醇溶液洗涤沉淀，可消除碘量法测定的干扰。

Soil organic matter, total nitrogen, available nitrogen and pH are measured with high temperature potassium dichromate oxidation-capacity, semiminim karl method, alkaline hydrolysis pervasion method, and the potentionmeter method respectively.

用五点混合取样法采集广西桂林毛村岩溶区和非岩溶区典型林地、灌草丛地和耕地的0~20cm，20~40cm土壤样品，以高温外热重铬酸钾氧化－容量法测定有机质含量，半微量凯氏法测定全氮含量，碱解扩散法测定有效氮含量，电位法测定pH值。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。