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Plasma insulin,glucagon and cortisol levels were measured by radioimmunoassay,and the levels o...

用放免法测定血中胰岛素、胰高血糖素和皮质醇的浓度,用ELISA的方法测定血中IL 6和TNF α的浓度。

Plasma insulin, glucagon and cortisol levels were measured by radioimmunoassay, and the levels of TNF-α and IL-6 were detected by ELISA.

用放免法测定血中胰岛素、胰高血糖素和皮质醇的浓度,用ELISA的方法测定血中IL6和TNF-α的浓度。

Double-antibody sandwich method for determination of 2 price or value of more than two macromolecular antigen, but not for the determination of haptens and small molecules unit antigens, because of two points can not form the sandwich.

双抗体夹心法适用于测定二价或二价以上的大分子抗原,但不适用于测定半抗原及小分子单价抗原,因其不能形成两位点夹心。

The feasibility of determining the pentosan in pre-extraction liquor from eucalyptus chips by using Douglas colorimetric method was discussed in this paper. The visible spectrums of xylose, glucose, mannose, galactose and kraft lignin were compared under the same color reaction conditions. The results showed that the disturbance from hexoses and lignin can be eliminated when the pentosan is identified and determined by the maximal absorption at 553nm.

摘 要:探讨了 Douglas 比色法用于测定桉木半纤维素预提取液中戊聚糖的可行性,通过对木糖、葡萄糖、甘露糖、半乳糖和木素在相同条件下显色反应后的谱图进行比较,证明利用553 nm 处的最大吸收进行测定可以排除己糖和木素的干扰。

Through tests on the determination of interference from the sample color, not only is its interference eliminated but also the accuracy of the method improved.

培酸法适用于深色溶液分析,灵敏度和准确度较高,通常用于总钒的仲裁分析;但是该法只适用于测定总钒,四价钒与五价钒只能用其它方法测定,这样就会引入方法误差,使四价钒、五价钒与总钒不匹配。

The uncertainty for determination of iron concentration in zinc-iron coating of galvaneal by chemical method was evaluated.

对用化学法测定锌铁合金钢板镀层铁含量测量结果的不确定度进行评定,分析了该方法测定过程的不确定

The results show that the maximum CLA content is 15.4 g CLA per 100 g isomerized rapeseed oil and the characteristic peaks of CLA in CLA standard as well as isomerized rapeseed oil are found at 234 nm at 25 ℃. A linear regression equation is obtained.

4g,利用紫外分光光度法测定的共轭亚油酸标准品和异构化菜油在25℃条件下的最大特征吸收峰波长为234nm,并建立了线性回归方程,因此,只要测定油样在234nm的吸光度再依此方程可算得油样中共轭亚油酸的浓度。

This paper has carried out careful thorough research according to the EDTA basic principle of the content of zinc in titration mensuration lead zinc mine, for its mensuration condition , has given steady, reliable analysis method and operating step , can offer guidance and basis for the inspection of lead mine in mining, metallurgical profession laboratory.

本论文根据EDTA滴定法测定铅锌矿中锌的含量的基本原理,对其测定条件进行了细致深入的研究,给出了稳定、可靠的分析方法和操作步骤,可为矿山、冶金行业化验室中铅锌矿的检验提供指导和依据。

Decomposition rates determined by the latter approach solved the problem of underpredicting litter decomposition rates by mesh bag technique.

采用后一种方法测定的凋落物分解率可以解决尼龙网袋法测定分解速率结果偏低的问题。

Rresults from those studies can be summariazed as follow:(1) A preliminary study no correlationship between the UV-VIS reflection spectrum of tomato fruit surface and the lycopene amount of tomato fruit was undertaken. Based on observation from this study, a damage-free and in-situ detection technique of lycopene amount from tomato was developed with UV-VIS reflection spectroscopy;(2) The geometrical isomers of lycopene were separated successfully on C30-HPLC-PDA-ELSD. The absorption coefficients of Z-isomers were consequently dedermined. The geometrical isomer composition of lycopene could therefore be assessed. Those efforts formed a solid base to identify the natural product from synthetic compound of lycopene;(3) Lycopene resource from the fruit of Autumn oliver was proved to be available for industrial application. An extraction methos of lycopene from the fruit of Autumn oliver by supercritical carbon dioxide was developed at laboratory scale;(4) A method to extract lycopene by supercritical liquid with improved efficiency was developed; Data from this investigation suggested that a proper raw material pre-extraction process was important for a better extraction efficiency;(5) Variation in the geometrical isomer composition of lycopene during its metabolish in rat was assessed. A large amount of Z-isomers were observed. This variation took place in serum;(6) The absorption rate and accumulation in serum of natural lycopene in rat were assessed;(7) Ability to quench singlet oxygen by different geometrical isomers of lycopene was compared;(8) It was detected that whether natural lycopene had functions to regule blood-lipids and LDL antioxidation;(9) A C31 degradation piece of lycopene was found in the prostate gland of rat.

研究结果包括:[1]应用UV-VIS反射光谱法建立了番茄果实中番茄红素含量的无损伤、现场检测技术;[2]应用C30-HPLC-PDA-ELSD技术实现了对番茄红素几何异构体的分离,测定了不同顺式异构体的吸光系数,建立了天然番茄红素几何异构体组成的检测方法,为区分和鉴定人工合成品和天然产物奠定了基础;[3]证实了秋橄榄果实中的番茄红素资源具有工业开发价值,并建立了超临界CO_2萃取的实验室方法;[4]探索了提高番茄红素超临界流体萃取效率的方法,证明了物料前处理对提高萃取效率起了极其重要的作用;[5]观察了番茄红素在大鼠体内几何异构体组成的变化情况,发现了大量顺式异构体的存在,并证实了几何异构体组成在血清中发生了显著变化;[6]测定了天然番茄红素在大鼠体内的吸收率和在大鼠血清中的动态积累规律;[7]比较了不同番茄红素几何异构体淬灭单线态氧的能力;[8]检测了天然番茄红素是否具有调节血脂作用和抗低密度脂蛋白氧化的功能;[9]首次发现大鼠前列腺内存在番茄红素C31降解片段,并证实其仍具有淬灭单线态氧的能力。

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