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Induced activation of Rac1,Cdc42 signal transduction pathway, monitoring the transfer of living cells activated sheet pseudopodium emerged and filopodia obvious morphological changes,such as measured values or fluorescence imaging of fluorescence intensity over time to continuously reduce until almost see the determination or observation.

诱导激活的Rac1、Cdc42信号转导通路,监测到转染激活的活细胞中出现片状伪足和丝状伪足等明显的形态变化,测定荧光值或者成像的荧光强度随着时间推移不断降低,直至几乎测定或观察不到。

The left carotid artery was expose and placed over an ultrasonic flow probe.The brain flow volume was recorded with an ultra-sonic volume meter.The pursiness time of mice was observed to evaluate the effect of tianma xingnao capsule on cere-bral ischemia and anoxia.Methods The permeability of normal blood-cerebral barrier was investigated in mice.

目的 应用二道血流速度流量仪超声探头测定大鼠颈总动脉,以反映云南省昭通制药有限公司提供的天麻醒脑胶囊对脑血流量的影响;方法记录小鼠断头后张口喘气时间,观察天麻醒脑胶囊的抗脑缺血缺氧作用;小鼠尾静脉注射Evans溶液,测定脑组织Evans的吸光度以反映药物的血脑通透性。

Objective The arteria carotis externa was ligated,and arteria carotis interna was kept opening The left carotid artery was exposed and placed over an ultrasonic flow probe.The brain flow volume was recorded with an ultrasonic volume meter.The pursiness time of mice was observed to evaluate the effect of Erigeron breviscapus pellets on cerebral ischemia.Methods Born's method was used to investigate effects of Erigeron breviscapus pellets on rabbit platelet aggregation induced by adenosine diphosphate,arachidonic acid,and platelet-acti-vating factor,in vitro or in vivo.Antithrombotic effects of Erigeron breviscapus pellets was monitored accord-ing to Peter's method.

目的 应用二道血流速度流量仪超声探头测定鼠颈总动脉以反映云南植物药业有限公司提供的灯盏花颗粒对脑血流量的影响;记录小鼠断头后张口喘气时间,观察灯盏花颗粒的抗脑缺血作用方法采用Born方法测定灯盏花颗粒在体内外对AA、ADP及PAF诱导的家兔血小板聚集功能的影响;运用电刺激大鼠颈动脉血栓形成方法,评价灯盏花颗粒对血栓形成的阻抑作用。

Ruggedness of an analytical method is the degree of tolerance that the determinating result is not affected when there is small change in the operational condition.

Ruggedness 耐用性指测定条件有小的变动时,测定结果不受影响的承受程度。典型的变动因素有:被测溶液的稳定性,样品提取次数、时间等。

Using of matrix assisted laser desorption/ionization time of flight mass spectrometry coupled with carboxypeptidase Y digestion for the analysis of C terminal peptide ladder by direct determination of the mixture of truncated peptide, the sequence of C terminus can be read out simplely from the mass differences between adjacent peptide peaks on the mass spectrum.

用羧肽酶Y水解蛋白质和多肽,结合飞行时间质谱测定酶解后缩短肽的质量,根据相邻两肽峰的质量差别读出蛋白质和多肽的C端序列,在pmol水平下测定了人促肾上腺皮质激素片段以及人血管紧张肽片段,其中人促肾上腺皮质激素片段测到了C端 15个氨基酸残基的顺序

Urushiol compositions of Chinese lacquer samples from 60 single trees were determined by GC. Film properties including surface drying time, luster value, shock strength, adhesion and flexibility were tested according to standard methods for coating.

本文作者用气相色谱法测定了约60个单株生漆样品的漆酚组成;同时用涂料标准方法测定了这些样品的漆膜的表干时间、光泽值、冲击强度、附着力、柔韧性等。

Uses the strong sulfuric acid anthrone determination kelp polysaccharide the content, mainly uses the single factor experiment definite orthogonal experiment, makes the microwave radiation time, the liquid-solid separately compared to, the ph value as well as the extraction temperature to the kelp polysaccharide extraction quantity influence, determines the best experimental condition again by the orthogonal experiment.

采用浓硫酸蒽酮测定海带多糖的含量,主要利用单因素试验确定正交实验,分别做出微波辐射时间、固液比、ph值以及提取温度对海带多糖提取量的影响,再以正交实验来测定最佳实验条件。

R. ChinaAbstract:Objective To study antiradiation and antifatigue effects of a soft capsule mainly composed of cistanche,acanthopanax senticosus and jujube. Methods The mice were divided into four groups and then orally given soft capsule at doses of 0 .0g/kg.bw, 0.18g/kg.bw, 0.36g/kg.bw, 1.08g/kg.bw for a successive period of 30 days.The burden-swimming time , hepatic glycogen, serum urea nitrogen, lactic dehydrogenase of mice were observed. The mice were divided into four groups and then was orally given soft capsule at doses of 0 .0g/kg.bw, 0.18g/kg.bw, 0.36g/kg.bw, 1.08g/kg.bw for a succession of 21 days.

设立3个实验组,每天分别以0.18、0.36和1.08g/kg·bw剂量的软胶囊给小鼠灌胃一次,同时设立空白对照组,连续灌胃30d后,测定小鼠的负重游泳时间、肝糖原含量、运动后血清尿素水平、血乳酸曲线下面积等指标,以测定其抗疲劳作用;每天以上述剂量给小鼠灌胃,同时设立一个辐射对照组,连续灌胃21d后,不同指标选择不同的照射剂量,各组均以同一剂量γ射线全身照射一次,照射后继续给予样品。

Objective To study antiradiation and antifatigue effects of a soft capsule mainly composed of cistanche,acanthopanax senticosus and jujube. Methods The mice were divided into four groups and then orally given soft capsule at doses of 0 .0g/kg.bw, 0.18g/kg.bw, 0.36g/kg.bw, 1.08g/kg.bw for a successive period of 30 days.The burden-swimming time , hepatic glycogen, serum urea nitrogen, lactic dehydrogenase of mice were observed. The mice were divided into four groups and then was orally given soft capsule at doses of 0 .0g/kg.bw, 0.18g/kg.bw, 0.36g/kg.bw, 1.08g/kg.bw for a succession of 21 days.

设立3个实验组,每天分别以0.18、0.36和1.08g/kg·bw剂量的软胶囊给小鼠灌胃一次,同时设立空白对照组,连续灌胃30d后,测定小鼠的负重游泳时间、肝糖原含量、运动后血清尿素水平、血乳酸曲线下面积等指标,以测定其抗疲劳作用;每天以上述剂量给小鼠灌胃,同时设立一个辐射对照组,连续灌胃21d后,不同指标选择不同的照射剂量,各组均以同一剂量γ射线全身照射一次,照射后继续给予样品。

This thesis includes five parts as follows.1. The backgrounds and some relative concepts of this work are briefly introduced. The photochemistry of benzamides and compounds with thiourea functional group is also concisely reviewed.2. Total of fourteen benzamides with different substituted groups have been synthesized and structurally characterized. Better photoreaction time for that kind of compounds at given concentrations is confirmed by tracking its'UV spectrum and GC chromatogram. A representative dibenzophenone photoproduct has been determined by X-ray crystallography. The mechanisms of photoreaction of benzamide have been proposed.3. Total of seven N-chloro(phenylmethylenebenzenamines with different substituted groups and sixty-one thiourea derivatives have been synthesized and structurally characterized.

论文的主要内容如下:1、简要介绍本论文工作的研究背景,并对苯甲酰胺和具有硫脲官能团类化合物的光化学研究进展进行简要的总结。2、合成与表征了14个具有不同取代基的苯甲酰胺类化合物,利用紫外光谱和气相色谱确定了此类化合物较佳光照时间,测定了一个具有代表性光解产物的单晶结构,提出了苯甲酰胺类化合物的光反应机理。3、合成与表征了7个带有不同取代基的氯代苯甲酰亚胺中间体及61个硫脲衍生物,测定了两个具有代表性化合物的单晶结构。

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