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OBJECTIVES: To predict duration of azotemia and distinguish acute renal failure clinically from chronic renal failure by measuring the concentration of hair creatinine and analysing the correlation between Hcr and serum creatinine in 59 patients with acute renal failure and 64 patients with chronic renal failure .

目的:通过对59例急性肾功能衰竭(Acute renal failure,ARF)病人和64例慢性肾功能衰竭(Chronic renal failure,CRF)病人头发肌酐含量(Hair creatinine concentration,Hcr)测定、比较ARF组和CRF组病人就诊时30-40mm段头发肌酐含量之间的差别、对Hcr与血肌酐浓度(Serum creatinine,Scr)相关关系的研究,在临床上利用头发肌酐含量测定来判定肾功能衰竭持续的时间,进一步鉴别急、慢性肾功能衰竭。

Methods Fifteen dogs were randomly divided into 3 groups. Group A acted as the control group without CPB; group B and group C were subjected to cardiopulmonary bypass and crossclamping the aorta for 90 min. MP or saline was injected intravenously before surgery. Plasma levels of IL-1β、TNF-α、IL-6、IL-8 and IL-10 were measured at the following time points: before MP or saline were administered, aortic declamping, 30 min and 90 min, after aortic declamping. Immediately after the animals was killed, pulmonary arterial rings were obtained and mounted in organ chamber for assessment of endothelial function with endothelium-dependent or non-endothelium-dependent studies.

健康家犬15只,随机分为3组,A组:不进行CPB,作为非体外对照组;B组:麻醉后注射生理盐水10mL,常规全麻和CPB,主动脉阻断90min;C组:麻醉后静脉给予甲泼尼龙5mg/kg,其余处理同B组。B、C组分别在手术前及主动脉开放前、开放后30min和90min 4个时间点采动脉血,放免法测定血浆白介素-1β(IL-1β)、肿瘤坏死因子-α、白介素-6(IL-6)、白介素-8(IL-8)和白介素-10(IL-10)水平;手术结束时,A、B、C 3组均取右肺动脉段,固定于张力传感器,测定血管环内皮依赖性和非内皮依赖性扩张能力的变化。

The aim of this study was to explore the potential relationship between the enhancement of instant hemostatic function in vivo of cryopreserved platelets and its procoagulative related molecule activities. The ability of platelet binding factor Ⅴ, density of GPIb-Ⅸ-Ⅴ(CD42a) at platelet member surface were detected by flow cytometry, the clotting time induced by activated platelets were evaluated by coagulometer and platelet count, MPⅤ and PDW were measured by hemocytometer before and after fresh platelets were cryopreserved.

为了探索血小板冰冻保存后膜表面促凝血活性相关分子变化与冰冻血小板体内即刻止凝血功能增强之间的可能联系,用流式细胞术检测了新鲜血小板冰冻保存前后Ⅴ因子结合能力、血小板膜表面GPIb-Ⅸ-Ⅴ分子(CD42a)密度,用血凝仪测定激活血小板诱导血浆凝固时间变化,用血细胞计数仪测定血小板计数、MPⅤ和PDW。

In the improved process, the stand time of the colored complex is prolonged, measurement can be done between 20 min to 2h after colorization.

改进方法中形成的硅钼蓝络合物稳定时间长,可在显色后 2 0min到 2h之内进行测定,3个样品平行测定的相对偏差仅为 3 %,加标回收率为 95 %~ 10 3 %,最大相对误差为 5 0 %。

The effects of displacer concentration, flow-rate, loading amount and the composition of the sample on separation efficiency are studied.

保留时间、峰高重现性及回收率测定结果显示此方法用于五种磷脂的定性和PC、PE及PI的定量测定,具有较高的精确度。

MethodsBasket-stirring method was used to determine the dissolution rate by determining the dissolubility of puerarin at different time.

采用转篮法测定两者的体外溶出度,以葛根素不同时间溶出度为测定指标。

According to the method D1102-84,which is the normative method of biomass ash measurement,approximate analysis has been done with the straws of wheat,rice,corn,bean,durra and cotton as sample.

按照D1102-84灰分标准测定方法,对小麦、水稻、玉米、黄豆、高粱、棉花等几种秸秆样本进行了工业分析;在ZRC2000智能灰熔点测定仪上对不同秸秆进行了灰熔点测试;在不同的温度和停留时间下,在马弗炉上对不同秸秆进行了热灼烧试验。

The content of CrO〓 and Cr〓 in electroplate waste liquid was determined successfully with this method.

经绘制标准曲线确定Cr、Cr的线性范围分别为0.5~35μg/mL和0.5~15μg/mL,迁移时间重现性的RSD均为0.9%(n=7),峰面积的RSD分别为3.1%和2.5%(n=7),测定的检测限分别为0.05μg/mL和0.04μg/mL,并将该方法用于电镀废液中铬离子的测定。

An indirect spectrophotometric method was used to determine the content of lactide. The factors on the determination, such as the type of solvent, acid and alkali dosage, the time of aminolysis reaction and so on, were discussed.

采用间接光度法测定粗丙交酯中丙交酯的含量,讨论了溶剂类型、胺解反应时间、酸碱用量等因素对测定结果的影响。

The results summarized as follows:(1)The main pharmacokinetic parameters Tmax was about 2h, Cmax was 8.365, 7.519, 1.871, 6.485, 4.060μg/mL in blood, gill, kidney, liver, and muscle respectively, after oral administration at a dose of 30 mg/kg; while t1/2 were 8.180~23.249h. These showed that the NFLX absorption was rapid after oral administration of NFLX in Scophthalmus maximus, but the residence time in the fish was long.(2) T1/2 were 74.88~200.45h after oral administration at dose of 30 mg/kg for five consecutive days, which showed that the attenuation of NFLX in the fish body was slow and the residue concentration in the kidney was the highest.

另外,本文测定了大菱鲆成鱼体内NFLX的药物浓度,用MCP-KP药动学程序对所得的药物浓度-时间数据进行分析,结果表明:1单次混饲口灌(30 mg/kg体重日)NFLX,药物在大菱鲆体内的达峰时间为2h,血液、腮、肾脏、肝脏、肌肉达峰浓度分别为:8.365、7.519、1.871、6.485、4.060μg/mL,消除半衰期为8.180~23.249h,说明NFLX在大菱鲆体内吸收较迅速,但滞留时间较长。2连续五天混饲口灌(30 mg/kg体重日)NFLX,消除半衰期为74.88~200.45h,说明混饲口灌NFLX在大菱鲆体内消除缓慢,其中以肾脏中残留最显著。

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