测定时间

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of IVF-ET program and were cultured with M199 media plus various concentration of follicle stimulating hormone (FSH, 0% 0.5% 5% 10% 25% 50) or human chorionic gonadotropin(hCG, 0% 5% 10% 50% 75% 100IU/ml). The media were collected and PAPP-A in the media were measured by time-resolved fluoroimmunoassay.

将来自体外受精—胚胎移植的卵泡黄素化颗粒细胞分离纯化后，利用不同剂量促卵泡激素(FSH，0、0.5、5、10、25、50)或人绒毛膜促性腺激素(hCG，0、5、10、50、75、100IU／ml)刺激体外培养的颗粒细胞，收集培养液，采用时间荧光免疫分析法测定培养液中PAPP-A的量。

The nauplii were sampled after fed with NFLX at different does and different time, the concentrations of NFLX were determined by High Performance Liquid Chromatography.

将卤虫无节幼体分别放入0.5、1、1.5、2.0g/L的NFLX混悬液中强化，不同时间点取样，高效液相色谱法测定卤虫体内NFLX含量。

In Chapter Two, some methods such as electron microscope, DNA ending label and MTT measurement were used to studied the changes of apoptosis, necroses and cell vigour of leukaemia K562 cell trunk and HL-60 cell trunk irradiated with millimeter wave. Different effects of millimeter wave to leukaemia cell trunks and human marrow cells were observed clearly. Human marrow cells were not so sensitive to millimeter wave radiation as leukaemia cells.

第二章，利用电镜技术、DNA末端标记、MTT活力测定等方法，研究了毫米波对红白血病K562细胞株、人类急性早幼粒白血病细胞株HL-60及正常人骨髓单核细胞的细胞超微结构、细胞凋亡、细胞活力的影响，结果发现毫米波辐射对正常人骨髓细胞及白血病细胞的影响明显不同，正常人骨髓细胞所受影响较小，特别是功率密度小的毫米波对其无影响；而白血病细胞受辐射后发生显著的凋亡或坏死，细胞活力显著下降，该效应具有明显的功率窗和时间窗。

According to the bactericidal assay of quantitative vehicle, the bacteria on the ozonized vehicles and unozonized vehicles was washed, 50μl eluant was seeded on the TSA plates.

用碘滴定法测定打开臭氧发生器不同机组及不同时间时的臭氧浓度，按载体定量杀菌试验，将经臭氧作用的载体与相同环境下未经臭氧作用的载体上的细菌洗下，各取洗脱液50μl，接种于TSA平板并置厌氧箱（90％N2，10％CO2，37℃）培养24-48h后，进行活菌菌落计数。

The ore-bearing porphyries of the Gangdese porphyry copper belt in the southern Tibet plateau possess the essential characteristics of adakite and formed from partial melting of subducted Tethyan oceanic crusts under eclogite facies conditions.

本文用冲江和拉抗俄两个矿区含矿斑岩的黑云母和斜长石斑晶测定了它们的~(40)Ar/~(39)Ar年龄，结果表明两个矿区虽然相隔百余千米，但形成时间非常一致。

In this thesis, the phase diagram of Zn-Al binary system on 10~69.5 w% was reinvestigated by using differential scanning calorimeter, X-ray diffraction and pushrod dilatometer. The process of isothermal transformation during the aging of high-Aluminum-Zinc-based alloy was studied by using differential scanning calorimetry, and the aging behavior of the alloy was analyzed at the aspect of thermal analysis kinetics. The change of microstructure which the alloy was being heated was observed on high-temperature metallurgical microscope. The microstructure and wear-friction properties were compared among the alloys treated with several different heat treatment processes, and a model for heat treatment processes was proposed based on artificial neural network,so as to predict the relationship between friction coefficient and parameters of aging treatment such as aging temperature and time.

本文采用高温X射线衍射、示差扫描量热分析、热膨胀等方法重新测定含锌量范围为10％～69.5％的Zn-Al系二元相图，利用示差扫描量热分析法，研究高铝锌基合金时效等温转变过程，并从热分析动力学的角度分析高铝锌基合金合金时效行为；使用高温金相显微镜观察合金加热时的组织变化；比较几种不同热处理工艺对高铝锌基合金显微组织与耐磨性能的影响并利用人工神经网络建立高铝锌基合金热处理工艺模型，预测合金摩擦系数随时效温度及时间变化规律。

The antibodies need to be measured at more than one time point.

自身抗体要在不同的时间点测定。

Methods The air sample was collected in the impactive absorption pipette with water as absorbing liquid, then immediately analyzed by GC to retain the qualitative time and the quantitative apex height or area.

用水作吸收液，冲击式吸收管采样，样品直接进气相色谱测定，以保留时间定性，峰高或峰面积定量。

In the measurement of the hydroxyproline in the collagen,the traditional acid hydrolysis method is that under 110 ℃ the animal organism is hydrolyzed with 6 mol/L HCl,for 24 h.

在胶原的羟脯氨酸测定中，传统的酸法水解是在110℃下使用6 mol/L的HCl消解24 h，其操作步骤繁琐且所花时间较长。

The influence of oven drying and air drying was compared, and the gels variation of pectin which was conserved for different periods was measured.

比较了人工干燥和自然干燥两种方式对果胶的影响，并测定了果胶在保存了一段时间后胶凝度的变化情况。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。