测定时间

And Toona sinensis were steam-treated at temperature 70℃ and 90℃ for 4 h and 8 h. The radial gas permeability and tangential gas permeability of the steam-treated specimens were compared with those of non-steam-treated one.

处理条件为：杉木和马尾松木材的汽蒸温度分别为90、100℃，假水青冈和香椿木材的汽蒸温度分别为70、90℃，处理时间分别为4 h和8 h，并对汽蒸处理前后木材的弦向和径向气体渗透性分别进行了测定。

The PVT emulsion as a kind of flocculants was applied to waste water treatment of paper mill, and the precipitation time and transmittancy were measured.

将 PVT乳液用于造纸废液的沉降处理，通过对沉降时间和透光度的测定可知，PVT乳液的用量为 0 。0 0 8%时，处理效果最好

The results indicate that the method is simple and rapid for the determination of strong polar trigonelline in Trigonella foenum-graecum L. Furthermore, it significantly reduces the equilibration time compared with ion-pair liquid chromatography recorded in the Pharmacopoeia of China. This new method can be used as a valid method for the quality control of Trigonella foenum-graecum L.

结果表明所建方法分离效果好、快速简易，可以弥补中国药典中离子对色谱法平衡时间过长的缺陷，适用于胡芦巴药材中强极性胡芦巴碱的测定，为胡芦巴的质量控制提供了有效的方法。

HL-60 cell line was treated with As2S3 at different concentrations and different period,and cell growth was analyzed with trypan blue exclusion.

以不同浓度的As2S3在不同的时间处理体外培养的HL-60细胞株，细胞生长测定、形态学观察及流式细胞仪检测等多种方法，在体外研究As2S3对HL-60细胞凋亡和增殖抑制的作用。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

After operation, comparative to the unoperative side, the AChE of operative side increased to +19.4%(P.05) on the 4th day, decreased to-38.6%(P.01) on the 15th day, by the 60th day it increased to +61.3%(P.05) again.

为了探讨这些间题，本文应用组化方法进行染色，利用照像显微镜的曝光时间对AChE的染色强度作了相对量的测定。1材料和方法

After unsealing the samples were taken at different time for determination of quality.

启封后不同时间分别取样进行品质测定。

Under the unsterilized conditions,solution culture experiments were conducted toinvestigate the influence of collection times and interfering factors on the determination oforganic acids in root exudates.

在非灭菌条件下，通过溶液培养试验研究了收集时间和干扰因子对根分泌物中有机酸分离和测定的影响，建立了用阴离子树脂消除干扰离子的方法。

Owing to the variability of HVR1, the method to check anti-HVR1 antibodies need to cloning and expressing the gene of HVR1 of every sera and that poses some problem. For example, it needs lots of time and money, and the sample must come from viremic patients. It is urgent to acquire a broadly cross-reactivity HVR1 antigen to check the antiHVR1 Ab in the sera of HCV infected patients by ELISA.

由于HVR1的高变性，因此要想测定血清中抗HVR1水平，通常要克隆表达待测血清中的HVR1基因，但该方法时间长、费用高，并仅对HCV、RNA阳性血清适用，所以急需一种具有极高交叉反应性的HVR1抗原，才可通过ELISA对病人的HVR1抗体进行检测。

The main results are as follows:(1)In order to find out the catalysis character of pectinase and xylanse in bacillus circularise A_6 and exhibit their maxium catalysis rate,the optimum condition for determination of pectinase and xylanase activity in bacillus circularise A_6 was studied in aspect of pH,temperature,substance concentration,reaction time,anion concentration.

试验结果如下：(1)为探明环状芽孢杆菌果胶酶及木聚糖酶的催化特性，更好发挥它们的催化性能，本文从pH值、温度、底物浓度、反应时间、离子强度几个方面研究了环状芽孢杆菌A_6产生的果胶酶与小聚糖酶活性测定的最适方法。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。