测定时间

Experimental studies were conducted at the Yucheng Comprehensive Experimental Station of the Chinese Academy of Sciences during the period from March to June in 2003 . Respiration of a pulverous sandstone soil was studied under cultivation of winter wheat over a growth season . Soil CH4 was measured by the Static-Chamber method combined with Gas Chromatography technique .

本文利用静态箱／气相色谱分析的方法，在中国科学院禹城综合试验站，对华北平原冬小麦生长旺季期间麦田土壤中CH_4气体通量进行了测定，试验起止时间为2003年3～6月，得出华北平原典型冬麦田土壤是大气CH_4的弱吸收汇。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Factors , photo-induced restoration , leading to specific changes in the most of the hole peaks on TSC spectra, but without reappearing of the TSC peaks before PQ, and reillumination with PQ light.

在本研究中，首次在T=75K用PIR光照射后的热激电流测定了深能级空穴陷阱的填充率，并与在不同PQ光照射时间下的陷阱填充率比较。

Keywords : solubility parameters apparent solubility parameters chromatographic fingerprint total quantum geometrical average retention time aloe-emodin rheic acid archen physcione HPLC compatibility rule reform t of the single flavor TCMM.

cm-3/2。结论：HPLC指纹图谱总量几何平均保留时间法能同时测定多个成分及总量的表观溶度参数，这对研究中药的配伍溶解规律，单味中药剂改工艺研究奠定理论基础。

As assessed by rheometry, the rigidity of collagen gel, ranged from 10 to 120 Pascal, was elevated by age effects and lowered by sonication.

以流变仪来测定胶原蛋白凝胶的黏弹系数，其黏弹系数范围从10到120 Pascal，其系数随老鼠年龄增加而上升，随超音波震荡时间增加而减少。

In these studies, 2 microbes were adopted. The time of fermenting CBM by DT and JM funguses involved respectively 3 stages: 5 days, 10 days and 15 days. After the trial of fermentation, the content of ricinine and allergen was detected.

本研究所采用的微生物为DT菌和JM菌，其发酵处理蓖麻饼的时间均分别为5d、10d、15d，发酵后测定并比较了它们对蓖麻碱、变应原的去除率及蓖麻饼中营养成分含量的变化。

objectives: by building a long time and high-intensity and endurance-trained and exhausting exercise model in mice, testing the activities of gsh-px, mn-sod, cu-zn-sod, gsh, content of mda, serum cortisol, aldosterone, atrial natriuretic and the exhausting time, the effects of rubia cordifolia l on metabolism of free radical of heart are studied in trained mice.

摘 要：目的：通过分析耐力训练后一次力竭性运动大鼠部分自由基指标的变化来探讨补充茜草提取物抗氧化、提高机体抗疲劳能力的可能机制。方法：通过建立大鼠递增大强度耐力跑台训练实验模型，测定了大鼠力竭时间、心肌织某些生化指标和抗氧化酶活性一以及血清皮质醇、醛固酮、血浆心钠素影响。

The time of test is 12 weeks. The change of serumal CRP and IL-6 of the two groups patients were observed.

在治疗前及试验终点时间分别对两组患者血清中的CRP和IL-6进行测定。

Result: Schwann-like cells disperse in the hole of nerve graft at oneweek under Scanning Electronic Microscope, the processes of thesatellite-like cells adhere to the wall of graft.There was no statisticaldifference of activity of cells in both group(P＞0.05) using MTT. At 12weeks,the result of appearance, histology and TEM were similar, therewere no statistical difference in SFI%, NEP and quantitative analysis ofrecovery rate of myelinated fiber populations, diameter of myelinatedfiber and thickness of myelin sheat.

结果：1w后扫描电镜下可见接种的类Schwann细胞分散在材料表面的孔隙内，细胞形态为星状，细胞突起与支架管壁粘附；MTT法测定细胞活性示神经支架组在各时间点与对照组无显著性差异P＞0.05；12w时两组动物坐骨神经外观、组织学光镜观察、透射电镜观察结果相似、两组坐骨神经功能指数恢复率、神经电生理、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率等指标相比无显著性差异(P＞0.05)。

METHODSUsing orthogonal design, total flavonoids in extract was as evaluation index, and ethanol concentration, extraction time, ratio of material to solvent and extration frequency were as inspecting factors to screened the optimum extraction technique, and total flavonoids content in extract was detected by spectrophotography.

方法］采用正交设计法以提取物中总黄酮含量为评价指标，以乙醇浓度、提取时间、固液比及提取次数为考察因素，筛选翻白草的最佳提取工艺。应用分光光度仪测定提取物中总黄酮的含量。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。