测定时间

Blood samples were drawn during different time after onset of chest pain in 60 patients with UA, 30 patients with stable angina pectoris and 30 patients with acute myocardial infarction. Blood was sampled only once per person in 30 normals. CKMM subbands were detected by the nonserial buffer electrophoresis method,and CK as well as CK-MB by velocity method. Student′t test was used for statisical analysis.

对60例UA、30例稳定型心绞痛和30例急性心肌梗死患者在胸痛发作后不同时间采血样(30例对照组仅采血1次)，采用不连续缓冲体系电泳方法检测CKMM3/MM1比值和用速率法测定CK同工酶和CK变化。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

The optimum cultivation time of several funguses fermented on the substrate of straw, lees, pachyrhizus and under the condition of pH7 and 28C was determined by mensurating the content of saccharide, the cellulose activity and the FP activity with the change of the time.

中文摘要：通过微生物共发酵的方法对发酵后产物滤液中还原糖含量、纤维素酶活力、滤纸酶活力随时间变化的测定，确定了BH菌种和实验室保藏的五种真菌在pH7、温度为28℃的条件下，在稻草、酒糟、地瓜干培养基上进行发酵的最佳培养时间。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

The method of series rate is used to study the linear growth order of ammonium paratungstate crystal.

将结晶成核期后晶体的生长阶段作为线生长速率测定的时间范围，采用&串联速率法&研究了仲钨酸铵的晶体线生长级数。

The character of hemagglutination of the virus could be inhibited by the antiserum of Newcastle disease virus.According to the international standard and method of virulence judgement of NDV, we measured the average death time、the minimal lethal dose and the median infective dose(EID50) of chicken embryos and the intracellular inoculation pathopoiesis index of 1-day-old chicks.

参照国际上规定的新城疫病毒毒力判定标准及其方法对分离毒株ZH1、ZH2进行了鸡胚最小致死量和平均死亡时间、鸡胚半数感染量(EID50)以及1日龄鸡脑内接种致病指数测定，结果ZH1、ZH2株的MDT为52h和44 h，EID50为106.4/ 0.1mL和108.64/ 0.1mL，ICPI为1.93和1.975。

METHODS: the supernatants of cultured spleen's lymphocytes induced by ConA were injected into mice peritoneally,kinetics of plasma corticotropin releasing hormone,adrenocorticotropic hormone and corticosterone in mice were tested at different time (0.5,1,2,4,6 h),or tissue of hypothalamus pituitarium or adrenal was stimulated with supernatants induced by ConA,and the secretion of CRH, ACTH and CORT were observed.

采用ConA诱导淋巴细胞培养上清液给小鼠腹腔注射，测定不同时间(0.5，1，2，4，6 h)的小鼠血浆中CRH，ACTH，皮质酮产生的动力学，同时在体外用ConA诱导上清液分别与下丘脑，垂体，肾上腺组织共同培养后观察CRH，ACTH，CORT分泌情况。

The nutrient value of Pholiota adiposa mycelia cultivated at 25℃, pH=6.0 and cultivated for 4d was higher. The amino acids score, chemistry score, essential amino acids index, biological value, Nutritional index and score of ratio coefficient of amino acid of its protein were 91.0, 70.6, 88.36, 84.61, 40.34 and 78.75 respectively.

本文测定了不同培养时间和PH值的黄伞菌丝蛋白质的含量及其氨基酸组成，并采用国际上通用的评价方法对不同培养条件的黄伞菌丝蛋白质营养价值进行了全面的评价，为黄伞资源的开发利用和科学研究提供可靠的理论依据。

Effects of culture time on the luminescence and growth of Photobacterium leiognathi was first reported in China.

从青岛近海分离鉴定出1株鳆发光杆菌YL，发光性质及其发光规律曲线的测定表明，鳆发光杆菌发光规律稳定，持续发光时间长，发光强度大。

The fresh chicken feces were obtained from different age 18 healthy chicken, and were not detectedchlortetracycline. The mixture standard solution of CTC and OTC was added in fresh chicken feces at the concentration of 0.5, 1.0 and 4.0ug/ml respectively. The chicken feces were divided to two groups,one were put in the incubator under photophobic condition; another were under the natural illumination.Samples were collected after 0, 0.5, 1, 2, 3, 5, 7, 9, 12 and 15 days, The concentration of chicken feceswas determined by HPLC. According to the theory of degradation kinetics, we fitted the changed trendof concentrations in chicken feces under different time and studied the degradation characteristics ofchlortetracycline in chicken feces.

选取不同日龄健康鸡18只，实验期新鲜鸡粪，，经检测不含四环素类药物，然后加入金霉素标准液，使得各样品中制得的相应药品的浓度为0.5ug／mL、1.0ug／mL、4.0ug／mL，分为两个实验组，第1组放入培养箱中避光黑暗条件下；第2组置于室外自然光下，定时取样，取样时间为0、0.5、1、2、3、5、7、9、12和15d，鸡粪中药物的浓度用本实验建立的高效液相色谱法测定，根据药物降解动力学原理，对鸡粪中药物的浓度随时间的变化趋势进行曲线拟合，研究金霉素在环境鸡粪中的降解动态规律。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。