测定时间

The main factors affecting the accuracy of Permanganate index determination are water bath calefaction time, titration time and blank value.

影响高锰酸盐指数测定准确性的主要因素是水浴加热时间、滴定时间和空白值，本文通过实验考察这三个因素对高锰酸盐指数测定的影响程度，对高锰酸盐指数的准确测定具有指导意义。

Methods:The levels of plasma PT and APTT under the different conditions were respectively detected with TECOhalf- automatic coagulometer made in Germany.

结果：不同温度下标本放置时间对PT、APTT的测定结果有影响，PT在20℃、37℃放置6小时测定结果差异有显著性(P.05)；APTT在20℃、37℃放置4小时测定结果差异有显著性(P.05)。

The result indicated that the percentage of lymphocyte were decreased, the percentage of neutrophile granulocytes were magnified significantly with the lighting time increasing, which was due to the level of the melatonin inhibited in the long time lighting; and to supply different exogenous dose, induce the percentage of lymphocyte were magnifying, the percentage of neutrophile granulocytes were decreased. And the statistical analysis showed there was significant difference.

结果表明：在给予不同光照时间的条件下，随着光照时间的延长，褪黑素的分泌水平受到抑制，导致具有特异免疫功能的淋巴细胞百分率下降，具有非特异免疫功能的中性粒细胞百分率上升，经统计分析表明不同组间的测定结果均存在显着差异；采用24h全天光照，用以模拟机体切除松果腺的情况，再给予一定剂量外源性褪黑素，导致机体内具有特异免疫功能的淋巴细胞百分率上升，具有非特异免疫功能的中性粒细胞百分率下降，经统计分析表明不同组间的测定结果均存在显著差异，并且高剂量褪黑素对机体的影响更为明显。

Methods The left ventricular diastolic function,the blood speed during quick filling phaseof left ventricular of the mitral valve orifice,the peak value of the late phase of left ventricular diastolic pariod;E/A ratio,the time for isovolumetric relaxation phase of left ventricular,the reducel time of peak E,and the distance between peak E of mitral valve and intervertricular septum of left ventricular were measured respectively.

摘要］ 目的应用彩色多普勒超声心动图测定冠心病左室舒张功能，临床提供可靠的诊断及治疗依据方法分别测定冠心病组及对照组的左室舒张功能：二尖瓣口左室快速充盈峰值血流速度即E峰；左室舒张晚期峰值速度即A峰；E/A值，左室等容舒张时间，E峰减速时间，二尖瓣叶E峰与室间隔左室面的距离。

By using the silicomolybdic yellow colorimetric method, the paper determines not only the existing form of soluble glass in the newly made-up, being used, and discarded cooling liquid,but also the relation of the stability of cooling liquid to the concentration and standing time of monosilicic acid and total silicic acid. The relation of the concentration of polysil...

本文采用硅钼黄比色法测定新配制、使用中和报废冷却液中的水玻璃存在形态和研究冷却液的稳定性与单正硅酸、总硅酸的浓度和放置时间的相关性；用四氢呋哺萃取比色法测定冷却液中的高聚硅酸含量与放置时间的相关性；研究用超声波、磁场等物理方法处理废冷却液的再生可行性及水玻璃的形态变化。

After administration,ketamine 50 mg/kg was peritoneally given for anesthesia, silk ligature thrombosis was used, then the wet thrombus was weighed for comparison of difference in thrombosis among the groups.

小鼠游泳耗竭时间的测定，灌胃给予中风安(6.0，24.0 g/kg)和脑血康(6.0，24.0 g/kg)及苯丙胺(0.2 g/kg，)和水。1次/d，连续灌胃5 d，于第5天给药后1 h测定小鼠游泳耗竭时间。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

In this way, much potential interference may be avoided in the determination of these drugs in biological materials and hemanalysis. These methods are rapid, simple and wider linear range, and can be used for determining procaine hydrochloride, dapsone and ampicillin sodium in injectin solutions of these drugs. The results obtained by these methods agreed with those by the official methods.A sensitive and selective method for the determination of dopamine was studied.

在此反应的基础上，实验(来源：6aABC论文网www.abclunwen.com)了pH值、表面活性剂、有机溶剂、加热时间、放置时间及干扰离子对测定的影响，所建立的方法具有线性范围宽，稳定性好等特点，可成功地用于相关药品含量的测定，与药典方法对照，结果吻合。

Meanwhile or 24 h later, 316 mg.l-1 sod was added to the culture to act for 24 h, and then, the intracellular reactive oxygen species, apoptosis ratio and expressions of mutant p53 and c-fos protein of hela cells were determined. results the production of ros and the apoptosis of hella cells increased as the action of ddp and adm continued within 24 h, but vp-16 failed to produce such effects.

宫颈癌hela细胞经顺铂、足叶乙甙（vp-16）和阿霉素血浆峰浓度不同时间作用后，测定肿瘤细胞内活性氧水平、细胞凋亡率及突变型p53和c-fos基因表达的变化，并测定不同时间加入sod对ddp、adm、vp-16诱导hela细胞凋亡及p53、c-fos基因表达的影响。

L^(-1) VP-16 for 6, 12, 24 and 48 h. Meanwhile or 24 h later, 316 mg?L^(-1) SOD was added to the culture to act for 24 h, and then, the intracellular reactive oxygen species, apoptosis ratio and expressions of mutant p53 and c-fos protein of Hela cells were determined. Results The production of ROS and the apoptosis of Hella cells increased as the action of DDP and ADM continued within 24 h, hut VP-16 failed to produce such effects.

宫颈癌Hela细胞经顺铂、足叶乙甙(VP-16)和阿霉素血浆峰浓度不同时间作用后，测定肿瘤细胞内活性氧水平、细胞凋亡率及突变型p53和c-fos基因表达的变化，并测定不同时间加入SOD对DDP、ADM、VP-16诱导Hela细胞凋亡及p53、c-fos基因表达的影响。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了