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To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量;薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用,确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化,流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

While the installation intends to record rain in the tangible form and trace all the possible factors which may influence the result of the performance such as the power and direction of the wind and the weight of the rain drops, etc.

地球外部作用于此时此地的因素,日出日落时间,太阳系的行星运动,银河系的变化,总星系中的平均质量密度,宇宙的场强、宇宙常数、哈勃常数的测定,膨胀系数和年龄,宇宙的中心与半径、红移等,把这些外界影响记录做出照片作为作品的一部分。

Methods Perfusion weighted imaging was performed in 45 cases of cerebral infarction. The sidetoside ratios of regional cerebral blood flow,regional cerebral blood volume and mean transit time were measured at duty lesion of ischemia. The hypoperfused cerebral areas and the stages of ischemia were defined as follows:compensation stage: rCBF was normal, rCBVwas increased, MTT was prolonged;decompensation stage: rCBF was slightly decreased, rCBV was normal or decreased, MTT was prolonged;preinfarction stage: rCBF and rCBV were decreased,MTT was prolonged.

利用磁共振灌注成像技术(perfusion weighted imaging,PWI)对45例缺血性脑血管病患者的责任病灶进行局部脑血流量,局部脑血容量,平均通过时间比值测定,确定低灌注脑区的影像学特点和缺血分期:代偿期(rCBF保持正常,rCBV升高,MTT延长);失代偿期(rCBF轻度下降,rCBV正常或下降,MTT延长);梗死前期:(rCBF下降,rCBV下降,MTT延长)。

The concentration was determined by LC/MS/MS and a concentration-time profiles could be obtained. The values of AUC were calculated with trapezoid method, t1/2 was calculated according to the last four points in the elimination phase.

采用液相色谱-质谱-质谱联用法,分别测定了给药后不同时刻比格犬血浆中氨苄西林钠和氨苄西林钠/丙磺舒钠浓度,绘制了血药浓度-时间曲线。

Methods Cassia Seed was soaked in chromium trichloride aqueous solution of different concentration (from 0 to 3600 mg/L) at 37℃ for 12 hours.

方法用不同浓度的三氯化铬水溶液,37℃浸泡决明子过夜,收获浸泡后的决明子,作为0h时间点的样品,然后分别收获24、48、72、96h的决明子芽,用石墨炉原子吸收分光光度法测定铬含量。

Blood samples from vein and artery end of the filter, plasma-based dialysate from entry and exit of the filter and ultrafiltrate were taken at 5 min(T0), 2 h (T1), 4 h (T2) and 6 h (T3) during HD-PBD and 5 min(t0), 6 h (t1), 12 h (t2) and 24 h (t3) during HVHF. Bilirubin levels of dialysate, ultrafiltrate and plasma were measured and quantity of serum bilirubin removed by extracorpomal circulation was calculated.

分别在HD-PBD治疗5 min(T0)、2 h(T1)、4 h(T2)和6 h(T3)及HVHF治疗5 min(t0)、6 h(t1)、12 h(t2)和24 h(t3)取滤器动脉端和静脉端血液,流入端和流出端透析液及滤出液,测定各时间点标本胆红素水平,并用公式计算其体外清除量。

We are satisfied that thefive main components were pure and plenty enough for NMR study. The structure and configuration of key component of urushiol weresuccessfully determined by using 〓 and 〓 conventioanl method,DEPTtechnique,homo- and heteronuclear chemical shift correlation 2D-NMR,2D-J resolved NMR,heteronuclear relayed coherence transfer 2D-NMR,and relaxation time measurement.The assignment of resonances for 〓and 〓 were confirmed.

对中国生漆含量最大的关键漆酚组分,在〓常规NMR谱和DEPT编辑谱的基础上,采用同核及异核化学位移相关二维谱,同核J分辨谱和异核中继相干传递二维谱等2D—NMR方法,并结合弛豫时间〓测定技术等,完全确定了占中国生漆总量60%左右的有两双键共轭的三烯漆酚的结构与构型。

The height of plant was measured every 15 days. The essential oil of the lemon verbena was obtained by steam distillation in different period of growth and analyzed by GC and GC-MS.

测定了株高变化,用水蒸汽蒸馏装置提取精油,用气相色谱仪和气相色谱质谱联用仪分析了不同时期精油含有率与成分的变化,对比不同器官、不同部位和不同采样时间的精油含有率的差异。

METHODS By the means of viscometry , the effects of reaction time , chitosan and acetic acid concentration on the hydrolytic velocity of chitosan by ultrasonic wave were investigated .

采用粘度测定法,探讨了超声波辐射时间与壳聚糖粘度的关系以及壳聚糖的浓度,醋酸的浓度对降解速度的影响。

Ubbohde's viscosimeter was used to examine the solutions treated by different times of irradiation under the action of certain Laser power to determine its viscosity.

以特定的激光强度、经不同的照射时间处理溶液,用奥氏粘度计测定溶液的粘度。

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