测定时间

The components in the Wuchai Particles and serum were analyzed by HPLC to monitor the changes of the schizandrin in serum so as to draw the curve of concentration connected with time . ResultsThe main component in Wuchai Particles was schizandrin ; after oral administration of Wuchai Particles , the schizandrin in serum of mice was detected .

方法小鼠灌胃给予五柴颗粒2 g/kg，在不同的时间点采血样，用高效液相色谱方法分析五柴颗粒和血清样品中五味子甲素成分，测定五味子甲素浓度的经时变化，绘制浓度-时间曲线图。

Methods Exerting backwards force on rats mandible and measuring concentration of rats' blood cAMP in three groups with radio-immunity. Drawing the time-concentration curve and making cosine analysis.

给大鼠下颌戴向后上牵引的矫形器，用放射免疫法测定不同加力组大鼠血中环磷腺苷含量，绘制时间-浓度曲线，并进行时间生物学的余弦统计分析。

MethodsThe model of cerebral ischemia and anoxia in mice was established by decollation and ligating common bicarotid artery the effect of BVF on the survival time of the mice,and the time of catching their breath after mice decollated,and mouse brain capillary vessel permeability were observed.

方法采用小鼠断头、结扎双侧颈总动脉等脑缺血缺氧模型，观察苦菜总黄酮对脑缺血缺氧小鼠存活时间、断头小鼠喘息时间及脑毛细血管通透的影响；TBA法、NBT法测定小鼠脑缺血缺氧后脑组织MDA含量和SOD活性。

Methods The model of cerebral ischemia and anoxia in mice was established by decollation and ligating common bicarotid artery the effect of BVF on the survival time of the mice,and the time of catching their breath after mice decollated,and mouse brain capillary vessel permeability were observed. The content of maiondialdehyde,activity of superoxide dismutasein the brain tissues of cerebral ischemia and anoxia mice were measured by TBA,NBT technology respectively.

采用小鼠断头、结扎双侧颈总动脉等脑缺血缺氧模型，观察苦菜总黄酮对脑缺血缺氧小鼠存活时间、断头小鼠喘息时间及脑毛细血管通透的影响；TBA法、NBT法测定小鼠脑缺血缺氧后脑组织MDA含量和SOD活性。

By using dipping enameling on a low carbon steel,effects of firing temperatures and time on structure and properties were studied,the hardness of the enamel coating was measured,and the relationship between hardness and firing temperature and time and the effect of crystal nucleus addition were also investigated.

采用浸搪法制备搪瓷基板，研究了不同烧结温度和时间对钢铁基板搪瓷制备工艺、组织和性能的影响，测定了搪瓷层的硬度，并分析了搪瓷层硬度与烧结温度和时间的关系，以及晶核添加剂对搪瓷层硬度的影响。

2Examine the chromatograms of the solutions to be examined in the assay. The two principal peaks in the chromatogram obtained with the test solution are similar in retention time to the principal peak in the chromatogram obtained with reference solutions of Estrone Sodium Sulfate and Equilin Sodium Sulfate.

在含量测定项下记录的色谱图中，供试品溶液两个主峰的保留时间应分别与对照品中雌酮硫酸钠和马烯雌酮硫酸钠峰的保留时间一致。

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果，对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾，经分析得出如下结论：1、本研究骨质疏松模型采用单一人工干预方法，没有其他影响因素；实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成，同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响；因此，同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中，模型组与对照组研究结果的差异表明：铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下，形成了一个互相影响的假设图：铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

Quiescent RASMCs were incubated respectively in 10% FBS DMEM as control group, 10% FBS DMEM supplemented with 0.1% dimethyl sulfide as vehicle group and 10% FBS DMEM supplemented with panaxynol(at concentration of 1,3,9×10-6 mol·L-1) as panaxynol groups. 1. Cell counting and double time. Cell viability was examined with trypan blue exclusion test and the cell number was counted at 0, 24, 48, 72h after the incubation using a hemocytometer.

实验中细胞分成如下几个处理组：①正常对照组：含10％FBS的培养液；②溶剂对照组：0.1％DMSO；③药物处理组：panaxynol 1、3、9×10-6 mol·L-1组。1、细胞计数及倍增时间测定：于接种并同步化后处理0、24、48、72h，用血细胞计数板计算活细胞数量，绘制生长曲线并计算细胞倍增时间。

METHODS Mices swimming time and enduring time under normal pressure in a hermetical container was calculated. Phagocytosis of mononuclear macrophage was determined with carbon particle clearance test.

以小白鼠游泳时间和常压密闭容器中死亡时间为指标研究抗疲劳及耐缺氧的药理作用；对小鼠碳粒廓清影响的免疫作用；急性毒性试验测定虫草洋参胶囊的最大耐受量。

Because of increased awareness of the activity and toxicity of current drugs, the threshold for initiation of therapy has shifted to a later time in the course of HIV disease. However, the optimal time to initiate therapy remains imprecisely defined.

随着对当前新药活性和毒性了解的不断增加，学术界比较认可抗逆转录病毒治疗开始的时间应该放在艾滋病患者病程的较晚期这一共识，但治疗开始的最佳时间尚未得到精确测定。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。