测定体积的

PAP-C23, amplified from mature pokeweed antiviral protein gene by PCR, was cloned into the expression vector PET101, and then the recombinant plasmid was transformed into Escherichia coli BL21(DE3). The recombinant mutant PAP was expressed high-levelly induced with IPTG.

用KCL溶液染SDS-PAGE胶，切下表达的目的蛋白，冰浴研磨后用磷酸缓冲液溶解，再加入等体积的福氏不完全佐剂，乳化完全后，免疫家兔，制备抗PAP蛋白的多克隆抗体，间接ELISA法测定所制备的抗血清的效价为1：1000。

The microglial cells appeared the ramified and rod figuration in the early stages of cerebral ischemia and in the border zone of infarct.

采用侧脑室注射缝隙连接特异阻止剂甘珀酸，测定其对脑梗塞灶体积的影响及对即刻早期基因FOS和GFAP表达的影响，以了解缝隙连接在脑缺血损伤中的作用。

It was more appropriate to define the posterior commissure as the posterior margin of HF volumetry, the determination of volume range of HF was helpful in lateralization of temporal lobe epilepsy. 3D MPRAGE was superior to SE in the volumetry of HF.

后连合作为HF体积测定后界较合适，HF正常值的制订有利于颞叶癫痫的定侧。3D MPRAGE序列较SE序列用于HF体积测定更为优越。

Methods Sixteen adult Sprague-Dawley rats were enrolled, from which anticoagulative erythrocyte suspension was made, and was incubate with equivalent volume of 1 time,2 times,4 times and 8 times of clinical therapeutic blood concentration of different local anesthetics respectively. Erythrocyte-yeast rossete and Tumor-erythrocyte rosette rate were measured.

选择成年SD大鼠16只，取抗凝血制备成所需浓度的红细胞悬液，与等体积的临床血药浓度、2倍、4倍及8倍临床血药浓度的各研究麻醉药物孵育后，加入补体致敏酵母菌或用自身血浆作为补体来源致敏的肿瘤细胞，测定红细胞、酵母菌花环率和肿瘤细胞-红细胞花环率。

We extract the lipid with hexane and then compare the removing effect of 20% trichoroacetic acid and 2.0mol/L chlorhydric acid after having condensed the extract.

将提取液浓缩后，利用正己烷萃取提取液中的脂溶性物质，然后分别测定在浓缩物中加入不同体积的20％的三氯乙酸及2mol／L的盐酸的除蛋白效果，结果显示盐酸为较佳的除蛋白试剂。

The errors caused by traditional methods in stem analysis can be significant, but these methods are still used in forest measuration.

讨论了树干解析中被忽略的2个问题，即直径和树皮体积的测定方法的严密性。

Densities of glycine and L-serine in aqueous LiNO3, NaNO3 and KNO3 solutions have been measured at 298.15 K with an Anton Paar Model 55 densimeter. Apparent molar volumes, limiting partial molar volumes and transfer volumes have been calculated. Hydration numbers and volumetric interaction coefficients for amino acids were evaluated.

用Anton Paar DMA 55精密数字密度计测定了甘氨酸和L-丝氨酸在LiNO3， NaNO3和KNO3水溶液中的密度，计算了氨基酸的表观摩尔体积、极限偏摩尔体积、迁移偏摩尔体积、理论水化数和体积作用系数。

A steel plate having a tensile strength of at least about 930 MPa (135Ksi), a toughness as measured by Charpy V-notch impact test at least about 120 joules (88 ft-lb), and a microstructure comprising at least about 90 volume percent of a mixture of fine-grained lower bainite and fine-grained lath martensite, wherein at least about 2/3 of said mixture consists of fine-grained lower bainite transformed from unrecrystallized austenite having an average grain size of less than about 10 microns and comprising iron and specified weight percentages of the additives: carbon, silicon, manganese, copper, nickel, niobium, titanium, aluminum, calcium,rare earth metals, and magnesium, is prepared by heating a steel slab to a suitable temperature; reducing the slab to form plate in one or more hot rolling passes (10) in a first temperature range in which austenite recrystallizes; further reducing said plate in one or more hot rolling passes (10) in a second temperature range in which austenite does not recrystallize, quenching (12) said plate to a suitable quench stop temperature (16); and stopping said quenching and allowing said plate to air cool (18) to ambient temperature.

一种钢板，其抗拉强度至少约930MPa135ksi），在－40℃（－40°F）下，采用夏氏V型缺口冲击试验测定的韧性至少约120焦耳（88英尺－磅），并且其显微组织包含至少约90％体积的细晶粒的下贝氏体和细晶粒的板条马氏体的混合物，其中该混合物的至少约2／3由从平均粒径小于约10微米的未再结晶奥氏体转变而来的细晶粒的下贝氏体组成，而且，其中所述的钢板包含铁和特定重量百分比的添加元素：碳、硅、锰、铜、镍、铌、钛、铝、钙、稀土金属和镁，所述钢的制备过程为：将一钢板坯加热至一个适当的温度；在奥氏体可发生再结晶的第一个温度范围内，采用一个或多个热轧道次（10），将所述板坯轧制成板材；在奥氏体不发生再结晶的第二个温度范围，采用一个或多个热轧道次（10）进一步轧制所述板材；将所述板材淬火处理（12）至一个适当的淬火终止温度（16）；停止所述淬火处理并将所述板材空冷（18）至室温。

Water samples were extracted and purified and collected by solid-phase extraction LC-18 column. Soil samples were extracted by ethyl acetate/petroleum ether (2:1, by vol) and purified by sulphonation. Wheat sample was extracted by acetonitrile/water (4:1, by vol) and purified and collected by SPE- Florisil column.

水样直接用LC-18固相萃取小柱分离、净化和富集；采用乙酸乙酯／石油醚（体积比2：1）振荡提取，磺化法净化，测定土壤中氯氟吡氧乙酸残留量；以乙腈／水（体积比4：1）为提取剂，氟罗里硅土固相萃取小柱分离、净化，测定小麦样品中氯氟吡氧乙酸的残留量。

Platelet membrane α-glucoprotein CD62p, CD63 expression and platelet aggregation function and mean platelet volume are indexes of platelet activation, which is a value to study about development of micrangium disease、early diagnosis and prevention in patients with diabetes mellitus.

CD62p、CD63的表达及聚集功能和平均体积的测定是反映血小板活化的指标，对判断糖尿病微血管病变的发生、发展及早期诊断和早期预防具有重要意义。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。