测定体积的

In this study, the CPE processes of four different kinds of polyaromatic hydrocarbons, acenaphthene, anthracene, phenanthrene and pyrene, with two kinds of silicone surfactants, DC-193 and DC-190, were studied separately, and four different assisting methods for the phase separation of the CPE process including heating at a higher temperature, adding salts, centrifugation and stirring were introduced, respectively.

今利用两种聚醚型有机硅表面活性剂DC-193和DC-190，对蒽、菲、芘和苊四种多环芳烃进行浊点萃取研究，分别使用加热、加盐、离心和搅拌等辅助方法改变相分离过程的速度。通过测定表面活性剂富集相的体积百分率Vs/随时间的变化和最终的萃取率，研究上述四种不同辅助方法对相分离行为和萃取性能的影响。

According to Dalton's law of partial pressures,the principle of experiment "Determination of the ideal gas constant R ",the volume and pressure of hydrogen produced in the experiment were analyzed and discussed.

从道尔顿分压定律出发，对教学实验"理想气体常数R 的测定"中产生的氢气的体积、分压和该实验的原理进行了详细的分析和讨论。

Feature and use: It tests melt flow rate of high polymer under mucosity flow state.It was expressed by MFR or MVR.It suits for testing polycarbonate,FI plastic and nylon under higher melt temperature. It suits for testing polyethylene、polystyrene、polypropylene、ABS colophony and polyformaldehyde colophony under lower melt temperature.

特点及用途：熔体流动速率仪是用来测定各种高聚物在粘流状态时的熔体流动速率，在一定的温度和负荷下，熔体每10min通过标准口模的质量或体积，用MFR或MVR来表示，它既适用于熔融温度较高的聚碳酸脂、聚芳砜、氟塑料、尼龙等工程塑料，也适用于聚乙烯、聚苯乙烯、聚丙烯、ABS树脂、聚甲醛树脂等熔融温度较低的塑料测试，广泛应用于塑料生产、塑料制品、石油化工等行业及有关院校、科研单位和商检部门。

By measuring the numerical of oxygen transfer coefficient in double bioreactor which in water, you can know whether the rate of oxygen transfer that is meet the oxygen consumption rate of the microbes so as to ensure that the microorganisms can grow.

通过测定摇床生物反应器的氧体积系数，就可以知道氧的传递速率是否满足微生物的耗氧速度从而确保微生物的生长。

In accordance with the method of silver nitrate titration of chloride in water,the sources of the uncertainty of measurement of chloride in water were analyzed and the uncertainty was evaluated.

根据硝酸银滴定法测定水中氯化物的含量，分析了该方法测量不确定度的来源，评定了水中氯化物的测量不确定度，在各不确定度中，以标准溶液配制与样品分析时滴定消耗的硝酸银体积引入的不确定度较大。

Our sensor not only can examine common solution, but also microsolution, the least volume can be 1-2μl. The most important feature of the electrode is that it can insert into organism, so detection in vivo is capable, and wide use is causable.

本传感器不仅能准确测定一般溶液的pH值，也能实现对体积仅1～2υl的微量溶液的检测，更重要的是这种针形金属pH电极能插入人体及其它生物体内，进行在体pH值的监测，其应用范围极其广泛。

The article reviews the advanced research on screening sheepbreeds with high resistance against fasciolosis. Screening sheep breeds with high resistance against fasciolosis has been tested by measuring parameter of plasma such as packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin concen tration, glutamate dehydrogenase, gamma glutamyl transferase and aspartate trans ferase and percent take of metacercariae. High resistance of Indonesian thin tail sheep against Fasciola gigantica has been confirmed. The mechanism of high res istance and the time of killing Fasciola gigantica has also been described.

本文综述了抗片形吸虫病绵羊品种的研究概况，通过红细胞压积、红细胞平均体积、红细胞平均血红蛋白浓度、血清谷氨酸脱氢酶、γ谷氨酰胺转移酶、天冬氨酸转氨酶等血液学参数，囊蚴感染成活率的测定等方法来筛选抗片形吸虫病的绵羊品种，研究证明印度尼西亚细尾绵羊对大片吸虫感染具有较高的抵抗力，并概述了其抗虫感染的机制及杀虫作用时间。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Department of Chemical Pharmacology, Capital University of Medical Science, Beijing 100054, ChinaAbstract: A reversedphase high performance liquid chromatographic method was developed for the simultaneous quantitative determination of caffeine, dapsone and chlorzoxazone. The operation was carried out on a C18 column (250 mmX4.6 mm i.d., 5.0 μm) with the mobile phase of acetonitrilephosphate buffer including 0.02 mol/L KH2PO4 and 0.02 mol/L (C2H53N, pH 6.5(25:75 in volume ratio).

用反相高效液相色谱同时测定血清中咖啡因、氨苯砜、氯唑沙宗探针药物的质量浓度，以乙腈磷酸盐缓冲体系(含 0.02 mol/L的磷酸二氢钾和0.02 mol/L的三乙胺，pH 6.5)(体积比为25：75)为流动相，以安替比林为内标，经C18柱(250 mmX4.6 mm i.d。， 5.0 μm)分离，紫外检测器检测，使3种探针药物得到较好的分离，并且在有效血药浓度范围内线性良好。

According to Dalton's law of partial pressures,the principle of experiment "Determination of the ideal gas constant R ",the volume and pressure of hydrogen produced in the experiment were analyzed and discussed.

从道尔顿分压定律出发，对教学实验&理想气体常数R 的测定&中产生的氢气的体积、分压和该实验的原理进行了详细的分析和讨论。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。