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Methods: Adenosine deaminase was purified by immtino-affinity chromatography, the enzyme activity was assayed colorimetrically by the measurement of ammonia produced over a fixed timeResults: The purified ADA was shown to be a homogeneity by polyacrylamide gel electrophoresis.

用免疫亲和层析法分离纯化人肾ADA,测氨法测定 ADA活性,经SDS-PAGE测定其分子量。

We identified positive phagocyte clone with ELISA, analysed its DNA sequences and homology comparition and detected the effect of phagocyte containing fused peptides on the multiplication of BmN cell with MTT.

利用 ELISA鉴定噬菌体阳性克隆并对其进行DNA序列测定和同源性比较分析、并利用 MTT法测定含结合肽的噬菌体对家蚕BmN细胞生长增殖的作用。

To eliminate the interference from K~+,the tests of cond...

为了消除K~+对Na_2O测定的干扰,进行了测定条件的试验。

Accuracy of determination of deacetylation degree of chitosan by two abrupt change conductometric titration was studied, and effects of chitosan solution concentration, viscosity and deacetylation degree on the result of equivalence point were also investigated.

结果表明:壳聚糖样品质量浓度控制在2~6g/L,利用双突跃电位滴定法可以测定壳聚糖的脱乙酰度,不同样品脱乙酰度测定结果RSD小于0.61%(n=3),该方法具有较高地准确性,可以用于壳聚糖生产和加工过程中的质量控制与检测

In this investigation,the composition of copolymer P(AM- co -NaAMPS) of acrylamide and 2-acrylamido-2-methyl-propanesulfonate sodium has been determined with the aqueous solution of cetyl trimethyl ammonium bromide as titrant at a concentration of 0.001 mol·L-1 by conductometric titration method.

与元素分析进行比较的结果表明,上述复合作用电导滴定法可作为测定水溶性大分子链中磺酸盐单体含量的方便而又准确的方法,而且预计还可用作为测定水溶性大分子链中其它离子性基团含量的简捷方法。

In vitro, the inhibitory activity of the fungicide against mycelial growth of both sensitive and resistant isolates of Fusarium graminearum was measured and the influence of the fungicide on conidial germination of Fusarium graminearum was determined.

离体条件下,采用菌丝生长速率法测定了该药剂对禾谷镰孢菌抗性菌株和敏感菌株的生长抑制活性;同时采用孢子萌发法测定了其对禾谷镰孢菌分生孢子萌发的影响。

It was demonstrated that when 0.10molL〓HCl was used as internal electrolyte, higher sensitivity and lower detection limit were obtained, and other copresent gases, such as CO, NO, NH〓 and CO〓, hardly had effect on responses of SO〓.

结果表明,以0.10molL〓HCl作为内充电解液,将此气体传感器用于测定二氧化硫,得到的检测限较低,且CO,NO,NH〓和CO〓等共存气体对二氧化硫的测定几乎没有影响。

The high correlation of CSP abnormalities with corticospinal tract dysfunction suggests supraspinal influence on CSPs.

我们的发现证实了CSPs测定在CCSM病例脊髓髓核内功能异常综合测定中的作用。

Methods compare with sysmex-r3500 analyzer、coulter maxm analyzer、miller ocularto determine 130 ordinary samples.results f=3.87,p.05,the difference is conspicuous.each"r"is 0.7331,0.7231,0.5011,each other are beeline correlation.about sysmex-r3500 analyzer,the inter-assay cv of quality control cv=2.95%,the sample is cv=4.42%;about couter maxm analyzer,the intra-assay cv is 5.70%,intra-assay cv is 17.02%;about miller ocular,the inter-assay cv is 26.15%.conclusion sysmex-r3500 analyzer is good at repeatability,convenience,quickness,so it is adapt to clinical batch samples quickly determine.

分别用sysmex-r3500分析仪,coulter maxm分析仪以及国际血液标准化委员会所推荐的miller窥盘法进行比较,对130份正常人标本进行网织红细胞测定。结果:f=3.87,p.05,差异有显著性。其两两之间r值分别是0.7331,0.7231,0.5011,两两之间直线相关。sysmex-r3500分析仪的质控物批内平均cv=2.95%,标本批内平均cv=4.42%,批间平均cv=3.42%;coulter maxm分析仪批内平均cv=5.70%,批间平均cv=17.02%;miller窥盘法批内平均cv=26.15%。结论:sysmex-r3500分析仪具有重复性好,简便,快捷的特点,尤其适合临床大批标本的快速测定。网织红细胞 miller;窥盘法;仪器法

This result is consistent with the measurement of CROM. Hence, our study develops the CROM be an objective instrument suitable to measure VDT operators' neck fatigue.

此与颈部量角器测定之工作后颈部活动度变差,有一致性的结果,同时发现本研究发展出使用颈部量角器测定法可作为VDT作业人员颈部疲劳的有效测量工具。

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