测定

The aims of the writers study is to observe the effects of its inducing apoptosis and influencing cell cycle by treating esophageal carcinoma cases with sodium cantharidate before operation.

本研究通过术前应用斑蝥酸钠注射液，旨在观察其诱导食管癌肿瘤细胞凋亡、对肿瘤细胞周期的影响，并初步探讨两者的关系；通过测定p53、bcl-2基因表达在应用斑蝥酸钠后的变化，初步探讨斑蝥酸钠治疗食管癌的分子机制；同时测定应用斑蝥酸钠后的免疫功能变化，以期对斑蝥酸钠对机体的影响做出较全面的评价。

Lipase mediates esterification between caproic acid (0.6mol/L) and ethanol (0.6mol/L) in n-heptane at 40℃. The decrease in caproic acid is measured by titration and 1 unit of lipase synthetic activity is defined as 1 μmol caproic acid consumed per min.

在40℃下，脂肪酶催化己酸(0.6mol/L)与乙醇(0.6mol/L)在正庚烷中进行酯合成反应，通过滴定法测定剩余的己酸，1个脂肪酶有机相合成酶活单位定义为：在测定条件下每分钟消耗1μmol己酸的酶量。

The cadmium stress was set up by 5 concn. treatments at 0, 0.5, 5.0、10.0, 50.0 mg/L to soak D. caryophyllus seeds and fresh leaves resp., the seed germination energy, leaf chlorophyll and isozyme were determined. The low concn.

方法］镉胁迫设5个处理(0、0.5、5.0、10.0、50.0mg/L)，对石竹种子浸泡后测定其发芽率；对新鲜石竹浸泡后测定其叶绿素含量和同工酶。

The main constituents C_1 and C_2 of Gentaminycin were evaluated in per centage by nuclear magnetic resonance spectrometry and comparison of pre - sa - turation water suppression with routine spectrometry of GTM matetrial drug was made to evaluate and analyse the GTM injections produced by 7 different factories.

用核磁共振谱(~1H-NMR)对庆大霉素的主要组分C_1和C_2的百分含量进行了测定，用预饱和抑制水峰谱与GTM原料药的常规谱进行了比较，并对7个厂家的GTM注射液进行了测定分析。

ResultThe maximumabsorption wavelength of reaction solution was about 720nm.The fermentation volume,adding the activated carbonor not,pHvalue of reaction solution and so on had no great effect onthe determinationresults and they could increase the screening efficiency.The effect of ultrasonic treatment for20min was obvious.When50ml volumetric flask was used,the suitable sampling volume was 2.5ml.It was better to centrifugate the fermentationliquid and the supernatant could be taken after standing inthe determination when necessary.

结果反应液最大吸收波长在720nm左右；发酵体积、加入活性碳与否、反应液的pH值等对测定结果影响不大，在筛选时可大大提高效率；超声波处理20min效果明显；在用50ml容量瓶进行定容时，取样体积以2.5ml为宜；对发酵液进行离心效果更好些，但在必要的情况下，可静置后取上清测定。

The optimal determining conditions were obtained: adding quantity of a monolayer medium 15ml, the suspension concentration of Micrococcus luteus 109 cfu/ml, the adding quantity of Oxford Plate 100μl, agar concentration 1%, the concentration of Na2HPO4 1％, and the pH of the medium adjusted to 7.0. The medium was optimized by single factor and the orthogonal experiments on the basis of the centrifugate of distiller's grains. The optimal composition was obtained as follows: sucrose 0.6, peptone 0.1, yeast extract 0.2, K2HPO4?

以管碟法为基础，对影响Nisin效价测定的各种主要因素进行了分析，并得出了Nisin效价测定的最佳条件：采用单层培养基（每个平板加量为15ml），检测培养基中Na2HPO4·12H2O浓度改为1％，菌悬液浓度109cfu/ml，不加吐温20，琼脂浓度1％，培养基pH7.0，牛津杯加液量100μl ，30℃培养24小时。

Effect of different surfactants on the UV intensity of Al(superscript 3+)-MXFX have been studied. Is Cetyl Trimethy-lammonium Bromide can increase the UV intensity of Al(superscript 3+)-MXFX is discovered. Based on this reaction, the reaction conditions in detail and proposed a simple, rapid and sensitive new method for the determination of MXFX by UV method is inve stigated. This method has been applied for the determination of MXFX in tablet with satisfactory results.

通过研究不同表面活性剂对Al(上标 3+)-莫西沙星体系紫外特性的影响，发现十六烷基三甲基溴化铵对该体系有显著的增敏作用；系统研究了CTMAB-Al(上标 3+)-MXFX反应体系的紫外特性，利用这一反应体系，建立了简单、快速、灵敏的测定莫西沙星的紫外分光光度法，选择了最佳实验条件，并将此方法用于莫西沙星片剂的测定，结果满意。

It has been identified whether chlorhydric acid or baicalin not. Chlorhydric acid and baicalin of preparation exist or not in by Tlc-scanning Method. This detective index could be used to control the quality of Shuanghuanglian Oral Liquid preparation. To determine the content of chlorphenamine maleate and baicalin by HPLC and UV-2000 and regulate the minimum limit as the control of preparation quality, in order to insure clinical management rationality, safety and usefulness.

通过薄层色谱鉴别口服液制剂中金银花、黄芩等药味的存在，并且金银花、黄芩分别以绿原酸、黄芩苷为考察指标，并采用HPLC、UV-2000测定金银花、黄芩进行含量测定，规定其下限作为制剂的质量控制指标，以保证临床用药的合理性、安全性和有效性。

Methods Determination of DNJ by derivatization with 9-fluorenylmethyl chloroformate and followed by reversed-phase high-performance liquid chromatography and a fluorescence detector; the inhibitory curves on α-glucosidase at the enzymatic level and the activities to α-aglucosidase in vitro were observed in the everted sac experiment.

以氯甲酸药甲酯柱前衍生RP-HPLC荧光检测法对DNJ进行分析；在酶水平测定样品的α-糖苷酶抑制曲线，在离体水平用反转肠囊实验测定样品的α-糖苷酶抑制活性。

Methods Serum contents of folic acid, vitamin B12 （VB12 ）, cholalic acid, interleukin（IL-2）, tumor necrosis factor, hyaluronic acid, type IV collagen, transforming growth factor-α, and cortisol were detected by radioimmunoassay and analyzed.

应用放免测定法对患者血清叶酸、VB12、胆酸、白细胞介素素-2（IL-2）、肿瘤坏死因子、透明质酸、Ⅳ型胶原、转化生长因子-α、皮质醇进行测定和分析。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。