测定

Change in the concentration of endogenous IAA was measured with enzyme-linked immunosorbent assay, and the distribution of IAA-binding protein in the protoplasts in the cambial region was tested with an immuno-fluorescence technique, being improved by the authors, during the cambial active period in Broussonetia papyrifera L.

构树（Broussonetia papyriferaVent。）形成层活动周期中用酶联免疫吸附法测定了形成层区域的内源IAA浓度的变化，并用自行发展的免疫荧光检测方法测定了由此处生活细胞制得的原生质体中IAA结合蛋白的分布。

The plant important value of Simpson and Shannon wiener in Jing zhou ancient wall are measured and illustrated. The important value of herb and liana and other relevant data are measured and illustrated. Pterocanfa stenoplera, Broussonetia,Papyrifera, Corydalis, Rabia,etc are selected.

测定了荆州古城墙植被中乔木的Simpson多样性指数值和Shannon-wiener多样性指数值，测定了草本与藤本等植物的重要值及其他相关数据，并得出B样方的植物多样性数值最高、I点的植物多样性数值最低等结论，筛选出枫杨、井栏边草、土牛膝、紫堇、薏苡等乡土植物。

In order to explore the mechanism of 'brumal jujube' of maturation and scenescence and ethanol accumulation, We not only studied the quality and the post-harvest physiological and biochemical changes of 'brumal jujube' after treatment with hormone during storage, but also investigated the effects of CAi (C027%~10%02+ 2~5%C02) and CA2:(7%~10%02, C02 2%) on the changes of fermentation volatiles, activity of enzymes , the kind and content of sugar and organic acid during storage in this paper. Firstly announced the reason of ethanol accumulation under higher C02 level and discussed the mechanism of ethanol accumulation during the end of storage.

本研究以冬枣为试材，采后采用激素进行处理，通过对贮藏期间果实品质、生理生化指标、果实衰老相关指标和酶活性的测定，系统探讨了外源激素处理对果实采后品质及衰老的调控作用；另外对点红冬枣采用CA_1：(7％～10％O_2+2～5％CO_2)和CA_2：(7％～10％O_2、CO_2≤2％)进行气调贮藏，通过测定冬枣果实品质、乙醇发酵相关酶活性及代谢产物、糖酵解底物及参与三羧酸循环代谢的有机酸含量和种类，研究了冬枣贮藏后期乙醇积累的机制，探讨了枣果不耐高浓度CO_2贮藏的原因。

The MTT assay was used to detect the viability of cells. Flow cytometry and laser confocal microscopy were used to identiyfy the cell cycle and apoptotic morphology after cells treated with LAO. RESULTS: An L-amino acid oxidase was successfully purified from the venom of bungarus fasciatus.

结果： 通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，L－氨基酸氧化酶的表观分子量通过SDS-PAGE测定约为60 kD，通过凝胶过滤测定分子量为70 kD.L－氨基酸氧化酶作用于HUVCE细胞12 h后，细胞的生长明显受到抑制，L－氨基酸氧化酶作用的半数抑制量为2.8 mg/L。

Different extracts prepared from Caesalpinia Sappan L were lavaged to the mice in order to count thymus index by taking out their thymus, to calculate the proliferation capacity of spleen T cells by using MTT method, to measure the INF-γcontent generated by T cell culture supernatant by using double-antibody sandwich ELISA method.

通过制备苏木不同提取物分别给予灌胃小鼠，取小鼠胸腺称重计算胸腺指数；采用MTT法测定小鼠脾T细胞的增殖能力；用双抗体夹心ELISA法测定T细胞培养上清中产生INF-γ的含量。

Objective To observe the comparability of results of serum enzyme determination by different instrument-reaget systems using enzyme calibrator.

目的 应用酶校正品校准不同的&仪器/试剂&测定系统，观察系统间血清酶测定结果的可比性。

Combined with optics, calorifics, electrics and acoustics, PA theory and PA application need deeply studied.

光声光谱的理论和应用，还需深入研究和开拓发展。光声光谱法适用性广，对于传统吸收光谱难以测定的不透明、高反射、高散射样品，也可以方便地测定。

The influence on physiques of Camponotus japonicus which were kept indoors in winter was studied. Their survival rate was counted. The activity of SOD and POD was tested by spectrophoto-meter.

研究室内过冬对日本弓背蚁生活力的影响，统计法测定了其存活力的变化，分光光度计比色法测定了野生蚁及低温处理蚁超氧化物歧化酶、过氧化物酶等指标。

Was studied by Folin Ciocalteu colorimetry. The results showed that polyphenol content of C. album could be well calculated according to their colorime tric absorption at 765nm by applying Folin Ciocalteu reagent 1.5mL and 10% Na2CO3 6 mL,2 h at 30℃ for colorimetric reaction, and the linear range of standard curve was 7-70mg/L.The method established was very convenient, stable and accurate.Key words: Canarium album Rauesch.;polyphenol;colorimetry

结果表明：橄榄多酚提取液在加入Folin Ciocalteu试剂1.5 mL，10% Na2CO3 6 mL，反应时间2 h，温度30℃的条件下进行显色，并于765 nm处测定其吸光值，多酚质量浓度在7～70mg/L范围内与吸光值有良好的线性关系，根据此线性回归方程进行橄榄多酚的定量测定，该方法简单方便，稳定性好，准确度高。

Rats was cannulated for in situ recirculation. UV and HPLC methods were used to determine the concentrations of phenolsulfonphthalein and scopoletin, respectively.

采用大鼠在体胃、小肠吸收实验模型，以高效液相色谱法测定灌注液中药物的浓度，紫外分光光度法测定酚红的浓度。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。