测定

Methods:The levels of plasma PT and APTT under the different conditions were respectively detected with TECOhalf- automatic coagulometer made in Germany.

结果：不同温度下标本放置时间对PT、APTT的测定结果有影响，PT在20℃、37℃放置6小时测定结果差异有显著性(P.05)；APTT在20℃、37℃放置4小时测定结果差异有显著性(P.05)。

The measuring device is structured by including a He-Ne laser; a beam expander, a soft aperture slot and a off-axis parabolic mirror to be measured are arranged in turn along the direction of the emergent laser beam direction of the He-Ne laser; a standard cross hair and a micro objective are arranged in turn on the direction of the reflected beam of the off-axis parabolic mirror; the micro objective is arranged on the focus of the off-axis parabolic mirror; the focus is imaged on an area array CCD; the output end of the area array CCD is connected with a computer; the off-axis parabolic mirror is fixed on a five-dimensional electric adjusting frame.

一种离轴抛物面镜点聚焦能力测定装置和测定方法，测定装置的构成包括：一台He-Ne激光器，沿该He-Ne激光出射激光束方向依次设置扩束器、软边光阑和待测的离轴抛物面镜，在该离轴抛物面镜的反射光束方向依次设置标准叉丝和显微物镜，该显微物镜位于所述的离轴抛物面镜的焦点，所述的焦点成像于面阵CCD上，该面阵CCD的输出端接计算机，所述的离轴抛物面镜固定在一五维电动调整架上。

A study was conducted in the lab from April to June in 2000. Mnipponense were fed in different vitamin D3 diets for four weeks and in vitamin D2 and D3 (70001U/ I OOg) for three weeks. After four weeks, we measured the effect of vitamin D3 in diet on survival, moult, length and weight gain, and the effect of the content of Ca P in plasma, in curst, in muscle, and also measured the effect of vitamin D3 on RNA/DNA value, on protein content in plasma and AEC of muscle.

本研究于2000年4-6月间在实验室条件下，用不同含量的维生素D_3投喂日本沼虾(Macrobrachium，nipponense)，饲养4周；用7000IU/100g饵料的维生素D_2和维生素D_3投喂日本沼虾，饲养3周后，测定了饵料中添加维生素D_3 对其存活率、蜕皮率、体长和体重的增长率等形态指标的影响；同时测定了虾血、虾壳和虾肌肉中的钙含量、磷含量以及维生素D对RNA/DNA值、血中蛋白含量和肌肉中的能荷的影响；并从上述指标的测定中，比较了饵料中添加维生素D_2和维生素D_3对日本沼虾生长的影响。

The growth, the lymphocyte proliferation and the content of serum corticosterone were determined with specific growth rate, thiazolyl blue deoxidize reactionand fluorescence method, respectively.

用特定生长率的方法测定小鼠的生长，用噻唑蓝还原法测定淋巴细胞转化，用荧光法测定血清中皮质酮的含量。

According to the characteristic that the isoflavonoids of Derris elliptica Benth have a distinctive absorption spectrum within the ultroviolet wave length range (210￣ 345 nm, 290￣305 nm), a new method for determining the total isoflavonoids in Derris elliptica Benth by Ultroviolet Spectrometry has been put forward.

根据鱼藤中异黄酮类化合物在紫外光210～245nm及290～305nm处具有吸收峰这一特性，提出了用紫外分光法测定鱼藤中异黄酮类总含量的方法。在300nm波长处，浓度低于6×2.618×10－3／mol·L－1时，测定了标准系列的吸光值，结果表明吸光值与浓度近乎直线相关，相关系数为0.9977；标准系列吸光值准确性高；加样回收率达99％以上；测定样品时，重现性好

The extent of nitridation is evaluated by the nitrogen content determined by a LECO Nitrogen/Oxygen determinator.

采用氮/氧分析仪测定产物氮含量，评价氮化反应程度；以BET氮气吸附法测定比表面积，分析粉体活性；以扫描电镜分析样品表面形貌；以热重分析仪测定碳含量，X射线能谱仪估计元素组成。

It is that:(1) the related compounds in Metronidazole Buccal Tablets prepared by Chongqing Medical College can be tested by determining (321nm) the total peak area of the impurities;(2) The related compounds in Metronidazole Disodium Phosphate for Injection prepared by Chongqing Medical College can be test by determining (254nm) the total peak area of the impurities and the peak area of the largest impurity;(3) Ceftriaxone polymer in Ceftriaxone Sodium for Injection prepared by Chongqing Keru Pharmacy Co.

结果如下：①用HPLC一UV法，在321nm处检测，用归一化法计算，控制各杂质峰面积的和，可检查重庆医科大学的注射用甲硝吟磷酸二钠中的有关物质：②用HPLC一UV法，在254nm处检测，控制杂质峰面积的和及控制最大杂质峰面积，可对重庆科瑞制药股份有限公司的注射用头饱曲松钠中的有关物质实施控制；③用HPLC一Uv法，在254nm处检测，按外标法计算，可检查注射用头抱曲松钠中的聚合物；④用紫外分光光度法，在277nm波长处测定吸收度，按C6H9N303的吸收系数为377计算每片标示百分含量，可测定重庆医科大学的甲硝哇口颊片的含量均匀度；⑤用紫外分光光度法，在277run波长处分别测定释放溶液20min、50min和90min的吸收度，按甲硝哇(C6H剥303)的吸收系数为377计算释放度，可检查重庆医科大学的甲硝哇口颊片的释放度。

When proceeding the research of drug identification by ultraviolet spectrophotometry, this paper adopts the approaches below: to identify Metronidazole Buccal Tablets and Potassium Sodium Dehydroandroan Drographolide Succinate by ulviolet maximum absorbent wavelength; to identify Metronidazole Disodium Phosphate for Injection by ulviolet maximum absorbent wavelength and minimum absorbent wavelength; to identify Ceftriaxone Sodium for Injection and Clarithromycin Tablets by retention time of high performance liquid chromatography- ultraviolet spectrophotometry.

通过测定紫外最大吸收波长λ_(max鉴别甲硝唑口颊片和炎琥宁、测定紫外最大吸收波长λ_(max和最小吸收波长λ_(min鉴别注射用甲硝唑磷酸二钠，结合高效液相色谱(high performance liquid chromatography-ultraviolet detector，HPLC-UV)中保留时间的测定鉴别注射用头孢曲松钠和克拉霉素片，本文建立了药物鉴别的紫外分光光度法。

Determine the content of the moisture with the dry law of the atmosphere; With is it draw law draw fat , analyse the backbone fat first esterification with the gaseous phase chromatogram to ask for; Determine the content of starch by fastenning all law; Propose the alcohol and sink the law and draw many candies with water, and determine many content of candy more than the color law with the phenol - the sulphuric acid.

用常压干燥法测定水分含量；用索氏提取法提取脂肪，将脂肪甲酯化，用气相色谱分析主要成分；用旋光法测定淀粉含量；用水提醇沉法提取多糖，并用苯酚-硫酸比色法测定多糖含量。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。