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Using of matrix assisted laser desorption/ionization time of flight mass spectrometry coupled with carboxypeptidase Y digestion for the analysis of C terminal peptide ladder by direct determination of the mixture of truncated peptide, the sequence of C terminus can be read out simplely from the mass differences between adjacent peptide peaks on the mass spectrum.

用羧肽酶Y水解蛋白质和多肽,结合飞行时间质谱测定酶解后缩短肽的质量,根据相邻两肽峰的质量差别读出蛋白质和多肽的C端序列,在pmol水平下测定了人促肾上腺皮质激素片段以及人血管紧张肽片段,其中人促肾上腺皮质激素片段测到了C端 15个氨基酸残基的顺序

Content of trypsin inhibitors in extracts of cyanobacterium from Taihu lake was determined by UV spectrophotometry in this paper.

采用紫外分光光度法对太湖蓝藻提取液中的胰蛋白酶抑制剂进行了含量测定测定的线性范围在 4~ 4 0 μg/mL 。

objective to study a modified method for detecting inhibitory activities of trypsin inhibitors purified from momordica charantia seeds.

目的用一种改进的测定方法,测定从苦瓜籽中提取分离的胰蛋白酶抑制剂的抑制活性。

Iron contents were determined by 1, 10-phenanthroline spectrophotometry and portable turbidimeter was adopted at the determination of turbidity.

铁的测定使用邻二氮菲吸收光谱法,浊度采用便携式浊度仪测定

It was studied that Apolipoproteins AⅠand B were determined by scattering immunoturbidimetric method using fluorescence spectrophotometer in stead of laser turbidimeter,and meanwhile the reference values of apolipoproteins AⅠand B of health persons were investigated.

研究了荧光分光光度计的免疫散射比浊法代替激光散射浊度仪来测定载脂蛋白AⅠ和载脂蛋白B,同时测定正常人的ApoAⅠ和ApoB的参考值。

Methods Among 86 patients in observation group with acute coronary syndromes,there were 47 cases with unstable angina pectoris and 39 cases with acute myocardial infarction,and 40 cases of health people in contrast group.The serum level of sICAM1 and sVCAM1 were detected by ELISA,and CRP was detected by immune turbidimetry in all cases.

选取ACS患者86例为观察组,将观察组分为不稳定型心绞痛和急性心肌梗死两组,UAP组47例,AMI组39例,另选健康成人40例为正常对照组,采用酶联免疫吸附法测定各组血中sICAM1和sVCAM1水平,采用免疫浊度法测定各组血中CRP浓度,并对其结果进行比较。

The various kinds of markers of whole blood viscosity and erythrocyte aggregation in the rats were measured with auto-viscometer,and the maximum rate of platelet aggregation was measured by turbidimetry.

采用反复注射肾上腺素和施以冰水浸泡的方法制作急性血瘀证大鼠模型,以全自动血流变学仪测定全血粘度和红细胞聚集指数等指标,以比浊法测定血小板最大聚集率。

Methods Twenty-two cases of nasal polyp and 10 cases of normal inferior turbinate were studied with immunohistochemical SABC method and ELISA method.

应用免疫组化和酶联免疫吸附测定法,对22例鼻息肉和10例对照组下鼻甲组织内IL-8 的分布及含量进行测定

RESULTS:The 50%ethanolic extracts of 20 Chinese crude drugs were examined for inhibitory activity on tyrosinase.

应用多巴色素法测定对酪氨酸酶的抑制活性,底物为L-酪氨酸,在490 nm处测定吸光度。

Through ultracentrifuge method the encapsulation efficiency was measured easily. Single factor investigation was carried out , and optimal formulation was decided .

建立了瑞利散射光测定微量NGF含量的方法;以超声法制备了小粒径的NGF脂质体;建立了超速离心法测定NGF脂质体包封率的方法;对影响NGF脂质体的包封率的因素进行考察,确定了最优处方。

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