测定

MAIN OUTCOME MEASURES: The seeded cells were observed under inverted microscope; at 1, 2, 3 weeks after seeding, the BMSCs were treated with 4% paraformaldehyde and stained with hematoxylin-eosin; The protein content in seeded cells was determined by bicinchoninic acid assay, and the content of DNA was quantified using Hoechst33258 assay at 5, 10, 14 days after culture.

主要观察指标：倒置显微镜下观察细胞形态；于培养1，2，3周采用40 g/L多聚甲醛固定，常规组织切片，苏木精-伊红染色；在培养5，10，14 d用Hoechs33258荧光法定量测定细胞内DNA含量，BCA法测定蛋白质含量。

Methods To determine the composition and activation of cerebroproptein hydrolysate by aminoacid analyzer,HPLC,SDS-PAG and succinate denydrogenase,to determine the therapeutic effectiveness of cerebrprotein hydrolysate by ordinary pressure oxytolerant experiment and paramnesia experiment.

用氨基酸分析仪、高效液相色谱仪、SDS凝胶电泳和琥珀酸脱氢酶法测定脑蛋白水解物的组成与活性。用常压耐缺氧实验和记忆获得障碍法测定脑蛋白水解物的疗效。结果：脑蛋白水解物的氨基酸与小分子肽的比例分别为 80 %与 2 0 %左右。

Methods To deter mine the composition and activation of cerebroproptein hydrolysate by aminoacid analyzer,HPLC,SDS-PAG and succinate denydrogenase,to determine the therapeutic e ffectiveness of cerebrprotein hydrolysate by ordinary pressure oxytolerant exper iment and paramnesia experiment.

用氨基酸分析仪、高效液相色谱仪、SDS凝胶电泳和琥珀酸脱氢酶法测定脑蛋白水解物的组成与活性。用常压耐缺氧实验和记忆获得障碍法测定脑蛋白水解物的疗效。

The method of using formaldehyde buffer solution a b sorption-hydrochloric pararosaniline spectrophotometry to determine sulfur diox ide in air has higher sensitivity and good selectivity,and prevents from adoptin g mercurial absorbent.But the conditions in lab are not well controlled,the v alue of reagent blank and standard curvilinear slope can't accord with the one s tipulated in"Methods for Air and Waste Gas Monitoring and Analysis".Some opra tional techniques for SO2 determination in lab are introduced for reference.

文摘：以甲醛缓冲溶液吸收-盐酸副玫瑰苯胺分光光度法测定空气中的二氧化硫，方法灵敏度高，选择性好，避免了使用含汞的吸收液，但如果实验条件控制不好，试剂空白值及标准曲线的斜率就会不符合《空气和废气监测分析方法》中规定的值，本文着重从操作技巧方面对二气化硫测定的实验室质量控制进行分析，仅供参考。

To determinate the inorganic elements levels in Semen Cassiae,and to study on the pharmacologic substance basis and the influence parching processing on the content of elements of it,trace elements and heavy metals in Semen Cassiae and its preparation Cultivated in different areas were determined by atomic fluorescence spectrometry and inductive coupled plasm a atom emission spectrometry.

为测定生决明子中无机元素水平，探讨其药效物质基础及炒制对其含量的影响，采用原子荧光法和等离子发射光谱法等测定了不同产地、批次的生、炒决明子中重金属及微量元素的含量。

Methods The left ventricular diastolic function,the blood speed during quick filling phaseof left ventricular of the mitral valve orifice,the peak value of the late phase of left ventricular diastolic pariod;E/A ratio,the time for isovolumetric relaxation phase of left ventricular,the reducel time of peak E,and the distance between peak E of mitral valve and intervertricular septum of left ventricular were measured respectively.

摘要］ 目的应用彩色多普勒超声心动图测定冠心病左室舒张功能，临床提供可靠的诊断及治疗依据方法分别测定冠心病组及对照组的左室舒张功能：二尖瓣口左室快速充盈峰值血流速度即E峰；左室舒张晚期峰值速度即A峰；E/A值，左室等容舒张时间，E峰减速时间，二尖瓣叶E峰与室间隔左室面的距离。

In the first part, more sensitive heat-sensor electrodes because of no electric insulator between the working electrode and thermistor are made for the first time and used to measure the electrochemical Peltier heat.

在第Ⅰ篇中，从实验和理论两方面提出了全新的测定电极过程热效应的方法，对测定中涉及到的问题提出了独立的见解。

A method for the determination of residual pentane content in de-oiled asphalt of Liaohe heavy crude vacuum residuum was studied by using static headspace gas chromatography. The effects of gas-liquid phase ratio, equilibrium time and equilibrium temperature on headspace analysis were investigated.

采用静态顶空气相色谱法测定辽河稠油减压渣油高软化点脱油沥青中戊烷的含量，确定了色谱的测定条件，研究了气液相比、平衡时间、平衡温度等因素对定量分析的影响。

Phenol method for methylation of sugar, pentose and polysaccharide determination method is simple and highly sensitive ...

下测定。苯酚法可用于甲基化的糖、戊糖和多聚糖的测定，方法简单，灵敏度高。。。。

The Trypsin inhibitor from Cucurbita pepo var. striaca Seeds was isolated by salting-out and chromatography of Sephadex G-50, the inhibitor activity was detected with BAPNA, the Mw was determined with SDS-PAGE and stablity was studied with different temperature, the side chains of Lys and Cys were modified.

采用分级盐析和Sephadex G-50的方法分离纯化无种皮南瓜籽胰蛋白酶抑制剂，用BAPNA法进行抑制活性的测定，用SDS-PAGE法测定蛋白质的分子量，用不同的温度处理方法对它的热稳定性进行研究，并对抑制剂的Lys和cys进行了化学修饰。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。