测定

The conclusions were as follows: theνO-H andνC=O IR absorption maxima shifted towards higher wave numbers after imprinting 17β-estradiol on TFMAA-co-TRIM copolymer with the red shift of theνO-H groups being apparent after precipitation polymerization for 16h while that forνC=O groups being observed after polymerization for 24h. A strong interaction between TFMAA and 17β-estradiol was confirmed by the high selectivity for 17β-estradiol, as indicted by the values of the separation factor of isomers of 17β-estradiol/17α-estradiol (2.28) and the imprinted factor (3.01). Particle diameter of TFMAA-co-TRIM polymer was between 300 nm and 1.5μm, which suited well for solid phase sorbent throughout at low column pressure. The recognition of imprinting TFMAA-co-TRIM polymer for sterol molecules was driven by enthalpy eluted with acetronitile, and low temperature was in favor of the separation of sterol structure analogues on imprinting column. TFMAA-co-TRIM polymer possessed of determinate anti-heat stability, with melting point beginning at 255.84℃, Tp=257.40℃, control TFMAA-co-TRIM polymer and imprinting TFMAA-co-TRIM polymer were respectively decompounded at 267.79℃and 343.11℃, and solid micro-extraction noddle prepared by the special polymerization also showed definite recognition for 17β-estradiol by GC/MS detecting at 270℃. By selection of various washing and elution solvents, elution reagents of close polymerization system were of more advantage in template molecules retention and recognition on molecularly imprinted solid phase extraction column. At a certain extent, progesterone, 17α-estradiol, 17β-estradiol and 4-androstene-3, 17-dione could be intercepted on the MISPE column. Especially, MISPE had high selectivity for progesterone, and imprinting TFMAA-co-TRIM polymer could achieve adsorption balance within 50 min by absorption kinetics test for 17β-estradiol. However, MISPE column showed better selectivity and enrichment property for 17β-estradiol than C18 and CSPE columns according to the data from HPLC and GC/MS analyses. Recovery of 17β-estradiol on MISPE column was up to 85.5% while when prime extracting solution of milk powder was sampled, the recovery of CSPE and C18 columns were 43.7% and 30.7%, respectively.

通过研究阐明：紫外聚合产物TFMAA-co-TRIM中的νO-H振动吸收峰在聚合16h后红移，νC=O振动吸收峰在聚合24h后红移；TFMAA-co-TRIM对雌二醇异构体的印迹因子达到了3.01，α为2.28，优于其它功能单体参与得到的聚合物识别特性；TFMAA-co-TRIM聚合物粒径介于300 nm至1.5μm之间，作为色谱固定相具有良好的通量和低的柱压；在乙腈流动相中，TFMAA-co-TRIM分子印迹固定相的分离过程主要被焓驱动，低温有利于分子印迹固定相分离甾醇结构类似物；TFMAA-co-TRIM分子印迹聚合物在255.84℃时开始熔融，Tp=257.40℃，聚合物CP的降解温度在267.79℃，MIP降解温度在343.11℃，制备的固相微萃取头初步经GC/MS 270℃的耐热性测定；不同的洗提溶剂筛选证明了选择接近聚合溶剂的洗脱体系更有利于MISPE发挥识别效应；分子印迹固相萃取柱对孕酮、17α-雌二醇、17β-雌二醇和雄烯二酮都具有不同程度的保留特性，可作为此类化合物的吸附材料，特别对孕酮强保留的富集特性，可作为孕酮的选择性识别吸附剂，其分子印迹聚合物对17β-雌二醇的吸附动力学测定显示在50 min内基本达到吸附平衡，具有作为传感器核心敏感材料的潜力；对比萃取奶粉中17β-雌二醇性能，MISPE柱比非分子印迹固相萃取柱和C18柱具有更高的保留，回收率依次为85.5%，43.7%和30.7%。

PartⅠ/chapter 3 The viability of cultured cerebral endothelial cells was determined by measuring the release of intracellular enzyme lactate dehydrogenase by nonviable cells with increased permeability.

第一部分第三章乳酸脱氢酶（1actate dehydrogenase，LDH）是一种细胞内酶，当细胞失去活力膜通透性增加时即释放到细胞外。本文通过测定释放到培养液中的LDH的量来测定牛脑微血管内皮细胞的活力。

We would like to make our students to know and master the basic way of the chemistry experiments, the use of the normal apparatus of inorganic and analytical chemistry, the character of the normal inorganic compounds and

课程的目标是使学生了解和掌握化学实验的基本方法和常见的无机与分析化学仪器的使用，了解和掌握一般无机化合物的性质以及分离提纯的方法；熟悉常见的无机化合物的制备方法，掌握测定有关重要化学常数的方法和原理；熟悉滴定分析的基本操作，掌握定量测定无机化合物的主要化学滴定的方法和原理，从而培养学生能用化学观点与方法来观察分析和解决实际问题的能力。

The plasma and serum were collected from patients of CHB and normal human,the correlation of the ET、histamine and Th1/Th2 cytokines was analysed.

以鲎试剂法测定血浆ET，以荧光法测定血浆组胺水平，分析ET、组胺与Th1、Th2类细胞因子的关系。

It is very significant and positive to search rapid methods of determination of nucleic acid.

核酸的定量测定是研究核酸的基础，目前核酸的测定方法有吸光光度法[2]、紫外光度法[3]、荧光分析法[4]和化学发光法[5]等。

METHODS:Nitrite was determined based on Griess reaction. iNOS mRNA was analyzed using Northern blot. NF κ B in the cell nucleole was detected with electrophoretic mobility shift assay.

Griess法测定细胞培养上清液NO-2水平以反映NO的生成，Northern印迹分析iNOS mRNA水平，EMSA法测定细胞核内NFκB的结合活性。

Used traditional measurement equipment and a new method-optimal approximation between measured and numerical calculation values, the coefficient of thermal conductivity of cornea was measured. It can be expressed by the equation of λ=0.10395+2. 75156×10〓t.

本文在不改变现有传统测定设备的前提下，采用一种新方法—通过数值计算角膜表面温度变化规律与实测值之间的最佳逼近，确定物体变物性导热系数的参数估算方法，对角膜的导热系数进行了测定，在角膜冻干的温度区间内，角膜的导热系数可近似地表示为λ=0.10395+2.75156×〓。

It consists of:(1) numerical filtering of both constant and variable parameters;(2) techniques of measuring group velocities by using "moving window" and "multiple filtering";(3) cross-correlation method of measuring phase velocities.

包括：(1)参数固定的和可变的数值滤波；(2)测定群速度的"移动窗"和"多重滤波"技术；(3)测定相速度的互相关法。

The determination method of gasoline rating units is the only reliable method to measuring octane number of gasoline.

汽油辛烷值发动机引法法测定法是目前唯一可靠的辛烷值测定法。

The feeding and excretion of three little fishes (Hemiculter. leucisculus, Tilapia. aurea and Cirrhinus. molitorelld) was studied and their effect on water quality of Qiankeng reservoir in Shenzhen City Around the topdown effect of fish was also studied. Based on the analysis of their stomach contents, their diet composition was assayed. The three fishes" feeding rhythm in cage and their rate of digestion indoor were looked into, and their daily ration was estimated through the method of daily average fullness index. Using the spectrophotometer, the content of chlorophyll a in the stomach was measured, and the feasibility of taking the chlorophyll a as an index of measuring the feeding of fish on phytoplankton was studied. Adopting the national standard method, the effect of the three fishes" excretion on total nitrogen, total phosphorus, CODMn, pH, turbidity and conductivity coefficient was surveyed. The effect of feeding and excretion offish on water quality was discussed and the results revealed as follows:H.

围绕鱼类的下行效应，本文以深圳茜坑水库三种小型鱼类Hemiculter leucisculus罗非鱼Tilapia.aurea和鲮鱼Cirrhmus.molitorella为研究对象，以胃含物分析为基础，分析了三种鱼类的食物组成；调查了网箱中自然状态下三种鱼的摄食节律，在室内调查了三种鱼的食物消化速率，运用日平均饱满指数法计算出三种鱼的日粮；采用分光光度法测定了鱼体肠叶绿素含量，探讨了叶绿素作为鱼类对浮游植物摄食量衡量指标的可行性；采用国家标准的水质测量方法，测定了鱼类排泄对总氮、总磷、高锰酸盐指数、pH、浊度和电导率的影响量。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新