测定

According to the bone apposition events at the interface, we measured the osteoblast attachment rate with the hemocytometer, the osteoblast growth curve with modified MTT method, the ALPase activity and protein content of cellular layers with modified kinetic method and modified Coomassie'method respectively, and osteocalain content in the cell medium with RIA , at different levels of cell responses, as osteocompatibility indexs.

根据骨内种植体界面的骨愈合过程，分别从成骨细胞反应的不同层次选择了细胞贴壁率、细胞生长曲线、细胞层ALPase活性和蛋白质含量以及细胞培养液中骨钙素的含量作为体外评价材料骨性生物相容性的指标。采用细胞计数板计数法测定细胞贴壁率，采用改良MTT法测定细胞生长曲线。

Methods To detect the contents of blood hemolysin in experimental mice,activity of NK cell,level of IL 2,and tumor necrosin in mice with EAC tumor.

对实验小鼠血清溶血素含量进行测定；同时，进行NK细胞活性及IL 2水平以及EAC荷瘤小鼠TNF含量的测定。

Methods The hesperidin content was determined by HPLC and analyzed by L8(2^7) orthogonal design and statistic analysis.

以高效液相法测定不同配伍中橙皮普含量，采用L8(2)'正交设计及SPSS统计软件分析测定数据。

Objective To study the cross contamination of alkaline picric acid in glucose determination by hexokinase method and its preclusion measures.

目的探讨肌酐测定试剂苦味酸对血糖己糖激酶法测定的交叉污染及排除方法。

Methods: The changes of surum-level T 3,T 4,FT 3,FT 4 and TSH in examinee by RIA were observed.The blood glucose were observed by methods of hexokinase.

用放射免疫法测定受试者血清 T3、T4、FT3、FT4、TSH的水平，用己糖激酶法测定血糖。

Methods: The chromatographic separation was performed on a Hypersil ODS2 column(250 mm×4.6 mm, 5 μm) with mobile phases of potassium dihydrogen phosphate hexyl amine solution-acetonitrile(74∶26) for the determination of its related substances and methanol-0.05 mol/L potassium dihydrogen phosphate solution(1∶1) for the determination of dexbetamethasone sodium phosphate. The flow rate was 0.8 ml/min, the detection wavelength was 254 nm, the column temperature was 25℃ and the sample size was 20 μl.

色谱柱为Hypersil ODS2柱(250 mm×4.6 mm，5 μm)；有关物质测定的流动相为磷酸二氢钾己胺溶液-乙腈(74∶26)，含量测定的流动相为甲醇-0.05 mol/L磷酸二氢钾溶液(1∶1)；流速为0.8 ml/min，检测波长为254 nm，柱温为25℃，进样量为20 μl。

The level of tumor necrosis factore alpha, interleukin-6 (IL-6) and interleukin-8 (IL-8) were evaluated using ELISA method. Western blotting was used to detect the protein expression of protein kinase B and lipid phosphatase PTEN. Immunoprecipitations and radioactivity of γ〓P incorporated into its specific substrate histon H〓B was utilized to determine the PKB activation. Phosphate concentration released from PTEN-specific substrat diC16PIP3 was assessed using green reagent method.

以细胞计数、噻唑蓝比色法测定细胞增殖能力，酶联免疫法检测培养上清液中细胞因子TNFα、IL-6、IL-8水平，Western免疫印记定量蛋白激酶B、蛋白磷酸酶PTEN表达水平，免疫沉淀、特异底物组蛋白H2Bγ〓P掺入量测定PKB活性，特异底物dic16PIP3脱磷酸绿色试剂法反应PTEN活性。

The effect of different infustion water methods to humidify coal seam were determined. The awash method and carve wall method were taken to measure the dust-adhering bar's function to prevent vaporization.

测定了不同注水方法润湿煤体的效果；分别采用浸润法和煤壁刻槽法测定了粘尘棒防蒸发性能；进行了普通注水与添加粘尘棒注水降尘的对比试验。

Results The sensitivity, specificity, and accuracy of PhaB-NRA for rifampicin were 89.1%, 91.67%, and 89.8%; on the contrary, those of isonicotinyl hydrazide were 86.21%, 90.0%, and 87.8%, respectively.

结果 PhaB-NRA法对利福平进行药物敏感性测定的敏感性、特异性、准确性分别为89.1%、91.67%、89.8%；对异烟肼进行药物敏感性测定的敏感性、特异性、准确性分别为86.21%、90.0%、87.8%。

The present invention relates to rhodamine 6G hydrazide salicylal azomethine, a synthesization method and an application in the content determination of cupric ions, which belong to the field of the analysis and determination of cupric ions in water sample.

罗丹明6G酰肼水杨醛希弗碱、合成方法及在铜离子含量测定的应用属于水样中铜离子的分析测定领域。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新