测定

HPLC, and its chemical structure elucidated on the basis of spectral analysis. A new method of RP-HPLC was established for the analysis of the contents of oleuropein, which is the main effective constituent. The contents of oleuropein in different positions of Syringa pubescens Turcz.

建立了测定橄榄苦甙含量的RP-HPLC法，并且利用这种方法测定了小叶丁香的不同部位——花、叶、茎和根之中的橄榄苦甙的含量；测定了不同条件下培养所得的培养物中橄榄苦甙的含量；测定了伴生菌培养物中橄榄苦甙的含量。

The pulse heating coulombic titration was applied to the determination of oxygen in titanium diboride,the titrimetry was studied to determine amounts of oxygen in titanium diboride,the determine method was established.

采用脉冲加热库仑滴定法测定二硼化钛中的氧，研究了二硼化钛中氧量的测定条件，建立了分析方法，能准确、快速地测定二硼化钛中的氧含量，并能推广应用于金属陶瓷材料中氧含量的测定。

We detected fasting plasma glucose by glucose oxidase,fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology,tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index,that is,ISI=1/FPG×FIns.

血糖测定采用葡萄糖氧化酶法；胰岛素测定采用化学发光法；血脂测定采用酶法；肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法；胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数，即ISI=1/FPG ×FIns。

We detected fasting plasma glucose by glucose oxidase, fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology, tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index, that is, ISI=1/FPG×Fins.

血糖测定采用葡萄糖氧化酶法；胰岛素测定采用化学发光法；血脂（甘油三酯TG、高密度脂蛋白HDL-C）测定采用酶法；肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法；胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数，即ISI=1/FPG ×FIns。

This research combine the physical therapy method of the Chinese native medicine ion to induct and though observe clinical case in recent years , refers to "the Chinese Pharmacopoeia","Traditional Chinese medicine dictionary ", in the external use the pharmacopoeia side, chooses 49 herbs commonly used Chinese medicine. Using the flame spectrophotometer to determines K, the Na content; using the atomic absorption spectroscope to determine the content of Mg, Ca, Zn, Mn; using acidometer and the chloride ion selective electrode to determine the content of Cl; using the spectrophotometer determines the content of S.

本研究结合近年来使用中药离子导入方法治疗闭合性软组织损伤的临床病例观察，参考《中国药典》、《中药大辞典》、100余种外用中药典方，选择49味常用中草药，用火焰分光光度计测定K、Na的含量；用原子吸收光谱仪测定Mg、Ca、Zn、Mn的含量；用酸度计和氯离子选择性电极测定Cl的含量；用可见分光光度计测定S的含量。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

S. bumalda from the Xiaochang County in Hubei Province was sunned to 90% dryness and dried to constant weight in the oven at 105 ℃, then grinded and its protein content was detected by micro-kjeldahl method, the soluble sugar content was detected by anthrone colorimetry, the crude fat content was detected by soxhlet extraction method, total ash content was detected by the method of burning with high temperature.

方法］将晒至9成干的湖北孝昌县珍珠花于105℃的烘箱中干燥至恒重，然后粉碎，凯氏定氮法测定其蛋白质含量，蒽酮比色法测定其可溶性糖含量，索氏抽提取法测定其粗脂肪含量，高温灼烧法测定其总灰分含量。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

Take DMSO swollen treatment and DEA-SO2-DMSO decrystallization treatment for example, the X-ray diffraction and the Tobolsky's intermittent stress relaxation of treated woods were determined during soaking in water, analyzed the effects of water on crystal degree and inter-cohesion of treated woods. According to these continuous relaxation curves measured in water with different temperatures, various thermodynamic quantities were obtained by using Eyring absolute rate theory, and reviewed the chemical reactions in wood which occur in different relaxation process. For the first time quantify these crosslinkings formed in the process of tensional relaxation by using the SMCIR intermittent stress relaxation way, and defined the cross-linking reaction types. In order to find out the contribute of drying to the fixation of deformation of chemically treated wood, stress relaxation of oven-dry untreated and treated wood was measured during the process of temperature elevation and descend, then analyzed the effect of temperature change on relaxation mechanism of treated oven-dry wood. According to continuous relaxation curves of oven-dry treated wood under various constant temperature, calculated the thermodynamics of relaxation process and discussed the mechanism of molecule change in wood, at the same time, also quantified these cross-linkings produced in wood by intermittent method and on the basis of which the model of molecular change during relaxation process of chemically treated was constructed.

以DMSO膨胀处理及DEA- SO2-DMSO非晶化塑化处理为例，测定了两种处理木材在水浸渍过程中的X射线衍射及Tobolsky间歇应力松弛，分析了水对处理木材结晶度及内部凝聚力的影响；通过未处理和两种处理木材在不同温度水中的连续应力松弛测定，应用Eyring的绝对速度反应理论计算并获得了松弛过程中的各热力学量，分析了在水中松弛过程中不同阶段木材内部发生的化学反应；并首次采用SMCIR连续·不连续双曲线应力松弛法定量了轴向拉伸应力松弛过程中木材内部产生的架桥量，明确了交联反应的类型；为了了解干燥对处理木材塑性变形固定的影响，测定了未处理和两种处理绝干木材在温度下降过程和上升过程中的应力松弛，分析了温度变化对处理绝干木材应力松弛的影响；根据多个温度水平下的连续应力松弛测定曲线，计算松弛过程的热力学量，考察了绝干木材在松弛过程中内部发生的分子变化机理，同时也用间歇法定量了木材内部新形成的架桥量，并在此基础上构筑处理木材在松弛过程中内部分子构造的变化模型。

Methods 1. The mice were divided into control and 3 Ganodermalucidum spores oil groups (at low, moderate and high doses), orally given forconsecutive 30 days. The body, spleen, thymus weight were observed, study on theeffect of the drug on the cellular immune function by proliferation and transformationof spleen lymphocytes test; study on the effect of the drug on the humoral immunefunction of mice by serum erythrocytolysin test; study on the effect of the drug on themononuclear macrophage function by carbon clearance and neutral red test.

实验方法 1设玉米油对照组和低、中、高三个灵芝孢子油剂量组，连续灌胃30天后观察小鼠体重、胸腺、脾脏重量指数；采用吞噬中性红和碳廓清实验测定对小鼠单核-巨噬细胞功能的影响；血清溶血素实验测定对小鼠体液免疫功能的影响；ConA诱导脾淋巴细胞转化实验测定对小鼠细胞免疫功能的影响；MTT法测定对小鼠NK细胞杀伤活性、脾细胞产生TNF-α和IL-2含量的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。