测定

Objective:To determine the content of gallic acid in liniment of compound Jin Yinhua.

目的：建立测定复方金银花擦剂中没食子酸的含量测定方法。

An Rp-HPLC method for the determination of gallic acid and ellagic acid in Sanguisorba officinalis L. is reported.

目的：建立RP-HPLC同时测定地榆提取物中没食子酸和鞣花酸含量的测定方法。

Can accelerate the bone marrow cell from G1 phase into S phase when we measure it's Cell cycle, it make S phase rate was increased .thus it promoted DNA synthesize and repaired. Mice irradiated at dose of 5.5 Gy gamma-irradiation showed timed-related decreases and restores in peripheral blood picture at day 1 through day 21, the counts of WBC of these group had taken drug has various degree to increase than irradiation control group in day 1. Grugs failed to protected the peripheral blood cell at lowest point, but seems promote their rescovery.9803 treated group was distinct. At 7days after mice were radiated in 3.5 Gy. we observed drug can Significantly hold back the decreased number of hematopoietic progenitors colony forming radiation induced. These findings indicated 9803 have a certain radioprotective activity against gammer irradiation in mice.

给药组的 CFU-S 数量较照射对照组明显增加，3.5Gy 照后第七天观察给药9803组对小鼠骨髓造血组细胞集落生成能力的影响，给药组的造血祖细胞集落的形成能力强于照射对照组，并且9803组明显优于523组。5.5Gy 照射引起的小鼠的外周血细胞数量的降低以 WBC 发生最早，照后24小时给药组的 WBC 数量均比照射对照组有不同程度的升高，以9803各给药组最为明显，药物未能使照射引起的外周血细胞最低值升高，而有降低的趋势，但给药对照射后期外周血象的总体恢复有较好的促进作用。7.5Gy 照射后第七天测定小鼠骨髓细胞周期的测定中发现9803具有促进骨髓细胞由 G1期进入 S 期的效应，致使 G0/G1期细胞显著减少， S 期细胞比率显著增加，这利于促进 DNA 合成修复，即促进骨髓细胞增殖，骨髓细胞 DNA 含量给药组显著高于照射对照组。

In the same time, they were determined with GC by controlling the temperature and the speed of nitrogen.

目的：建立同时测定生活饮用水中10种卤代烃的顶空气相色谱测定方法。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

Results and Conclusion The extracting rate was 57%, gel strength reached 560 g/ cm~ 2 , the agarose has good electricity function by measuring the gentian violet electrophoresis and the DNA gel electrophoresis, so it is suitable for bioche- mistry and molecular biology electrophoresis.

结果测定的灰分和硫酸根含量均符合电泳琼胶糖的指标，凝胶强度达到560g/cm2，提取率达57%，测定了其结晶紫电泳和DNA的琼胶糖凝胶电泳。结论本方法提取的琼胶糖具有良好的电泳性能，适合用于生物化学和分子生物学的凝胶电泳研究。

40Ar-39Ar method is the first one of the possible geochronology techniques to solve this difficult scientific problem.

油气成藏作用伴生的矿物种类少，主要为碳酸盐矿物，以及少量石英和黄铁矿等，这些矿物均不适合用传统同位素年代学方法进行年龄测定，因此，油气成藏年龄是同位素年代学尚未解决的一大科学难题。40Ar-39Ar法是可能应用于油气成藏年龄测定的首选同位素定年方法。

To study the spatial variability of the sandy desertification soil on the Erdos Plateau, the water content of top soil(0～30cm) under different landforms was measured, and analysed by geostatics methools; and; each layer's water content was measured on some typical soil profiles along the landform section.

为了研究鄂尔多斯夏初不同地形土壤水分的空间变异规律，本试验在鄂尔多斯高原几种地形的沙质荒漠化土壤上，用时域反射仪测定表层土壤(0～ 30 cm)的含水量，对表层土壤水分分布特征进行分析；在不同地形部位选择典型土壤剖面，测定各层土壤含水量。

The conditions for copper fire assay collection, electrode matching in glow discharge and mass spectrometric analysis were optimized.

着重考察了铜试金条件的选择和辉光放电电极的匹配、质谱的测定条件和测定方法等。

The reformed method was more accurate and reproducible. It can be used in quality control of glucosamine.

改进后的测定法凖确、重复性好，适用於含量测定。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新