测定

A data acquisition and data analysis software for the fast fluorscence kinetics fluorometer of our laboratory is redesigned and recompiled using assembly and BASIC language. The data acquisition rate in the fast rising part of the fluorescence kinetics curves is one order faster (100μs/point) than the old one. It can measure fluorescence parameters, such as Fo and Fi more accurately for calculation of the relative content of inactive PSⅡ, etc.

用N80-BASIC语言结合汇编语言重新编写了本室快速叶绿素动力学荧光计的测控程序，使快速荧光上升曲线的采样速度提高了一个数量级（达到100μs／点），可对Fo、Fi等关键荧光参数进行精确测定，为无活性PS Ⅱ中心相对含量的准确测定奠定了基础。

By researching the original analysis method for CaF2 in fluorspar,this paper discovered its shortcomings,determined three main factors for causing CaF2 analysis results to be lower slightly:determination of total Ca contents,determination of CaCO3 as well as calibrating of EDTA standard titration solution.

通过对萤石中 CaF2的原分析方法的研究，找出其中的不足，确定了导致 CaF2分析结果偏低的三个主要因素：Ca的总量的测定、CaCO3的测定以及EDTA标准滴定液的标定，结合国家标准分析方法，对我们正在使用的分析方法进行改进，以解决这些问题。

Measurement of Mg/Ca ratio of foraminifera shell is the most popular means in calculating seawater paleotemperature now. It is applicable in low and high latitude areas, to determine surface and deep seawater temperatures.

有孔虫壳体的Mg/Ca比值测定是目前估算海水古温度最受重视的方法，适用于低纬和高纬、表层和深层海水古温度的测定。

Forced oscillation is a convenient, quick, and noninvasive new method when applied to measure airway resistance and phase angle.

强迫振荡法是一种简便、快速、无创性测定气道阻力与相位角的新方法，适用于重症慢性阻塞性肺疾病患者，且对哮喘的用药观察优于传统肺功能测定。

In this work, we also presented some methods to determine kinetic parameters for the different systems and examplified by the reduction of thionyl chloride in dimethyl formamide.

根据我们提出的判定反应可逆性的方法，系统地对EE反应机理进行了探讨，从中可方便地判定EE反应的不同反应机理；对不同的反应机理，相应地又提出了测定其电极反应动力学参数的方法，并以亚硫酰氯的还原为例进行了研究及测定。

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段，序列测定表明除585位有一点突变外，所得suc2基因与文献报道相同，构建的定位突变载体导入酵母细胞后，得到4株营养型符合的突变体，PCR表明这些突变体中均有suc2基因的同源重组，取其中1株的PCR产物进行序列测定证实了同源重组的发生。

After 4 passages in culture, the MSCs were implanted into 96 foveola with cell density of 0.08,0.1,0.2,0.4,0.6,0.8,1,2,4,6,8,10(x104/ml) respectively. Meanwhile, the MSCs were cultured in Dulbecco's modified Eagle's medium culture medium with different concentration of fetal bovine serum, the 3-4,5dimethylthiazol-2-yl-2,5-diphenyl tetrazelium bromide assay were used to determine the proliferation index of MSCs 72 hours later, the cell cycle were determine by cytometer also.

贴壁筛选法分离、纯化MSCs，取4代以后细胞，以不同密度接种于96孔培养板内，分别以不同浓度血清进行培养，72h后，采用四甲基偶氮唑盐比色法测定MSCs增殖能力并用流式细胞仪测定细胞周期。

Methods: The sample extraction method of total flavonoids, including water bath water bath reflux extraction and ultrasonic extraction method, the sample determination of total flavonoids, including aluminum nitrate chromogenic method and direct color method, the sample determination of antioxidant flavonoids, including FRAP method and DPPH method.

样品总黄酮提取方法包括恒温水浴回流提取法和恒温水浴超声波提取法，样品总黄酮含量测定方法包括硝酸铝显色法和直接显色法，样品总黄酮抗氧化性测定方法包括FRAP法和DPPH法。

According to the papers EDTA Titration of lead in lead-zinc content of the basic principles Determination of its conditions for the in-depth and meticulous research, is a stable and reliable analytical methods and operating procedures, that will be for the mine, China Metallurgical Industry Galena laboratory testing to provide guidance and basis.

本论文根据EDTA滴定法测定铅锌矿中铅的含量的基本原理，对其测定条件进行了细致深入的研究，给出了稳定、可靠的分析方法和操作步骤，可为矿山、冶金行业化验室中铅矿的检验提供指导和依据。

ABSTRACT: Objective To investigate the effects of epigallocatchin gallate on telomerase activity of bladder carcinoma cell line T24 and BIU87 and its possible mechanisms.

目的 探讨茶多酚对膀胱肿瘤细胞株T24和BIU87的端粒酶活性影响及其可能机制。方法用MTT法测定治疗组、阳性对照组、阴性对照组的细胞抑制率，用流式细胞仪测定细胞凋亡率。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新