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Also, it could be used to study the effect of cellobiose on cellulase activities and the inhibition of dyestuff in the enzymatic treatment bath against cellulase activities.

滤纸崩溃法可以测定某些滤纸糖化法无法测定的中性酶和弱碱性酶的总活力,也可用来研究纤维二糖对纤维素酶活力的影响,以及染料溶液对纤维素酶活力的抑制等。

On different conditions, the results of the measurements of cellulase activity are different.

在不同的条件下测定酶活力会得到不同的纤维素酶活力测定值。

Methods Colloidal gold-labeled monoclonal antiCK20 antibody was absorbed on nitrate cellulose membrane, and then the membrane was fixed on plastic strip with double face glue as checking line.

方法将经处理的CK20单抗标记在胶体金颗粒上并活性进行鉴定,合格后吸附在硝酸纤维膜上并固定在长方形硬质塑料胶片一端为测定线;同时将吸附有IgG蛋白胶体金的硝酸纤维膜固定在测定线的一侧为质控线。

In the test range homogenizing speed and time and the centrifuge time all had small effect to the results.

测定的范围内均质速度、均质时间、离心时间对测定结果的影响都很小。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主,要求研究生学习和掌握一些现代微生物学研究所需的基本技术,内容包括:相差显微镜、荧光显微镜的使用和显微摄影技术,PCR技术的应用,细菌质粒的构建、转化及检测技术,病毒的侵染性及其分子检测技术,丝状真菌原生质体融合技术,大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术,细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

Methods Fifteen dogs were randomly divided into 3 groups. Group A acted as the control group without CPB; group B and group C were subjected to cardiopulmonary bypass and crossclamping the aorta for 90 min. MP or saline was injected intravenously before surgery. Plasma levels of IL-1β、TNF-α、IL-6、IL-8 and IL-10 were measured at the following time points: before MP or saline were administered, aortic declamping, 30 min and 90 min, after aortic declamping. Immediately after the animals was killed, pulmonary arterial rings were obtained and mounted in organ chamber for assessment of endothelial function with endothelium-dependent or non-endothelium-dependent studies.

健康家犬15只,随机分为3组,A组:不进行CPB,作为非体外对照组;B组:麻醉后注射生理盐水10mL,常规全麻和CPB,主动脉阻断90min;C组:麻醉后静脉给予甲泼尼龙5mg/kg,其余处理同B组。B、C组分别在手术前及主动脉开放前、开放后30min和90min 4个时间点采动脉血,放免法测定血浆白介素-1β(IL-1β)、肿瘤坏死因子-α、白介素-6(IL-6)、白介素-8(IL-8)和白介素-10(IL-10)水平;手术结束时,A、B、C 3组均取右肺动脉段,固定于张力传感器,测定血管环内皮依赖性和非内皮依赖性扩张能力的变化。

To determine and analyze the contents of amino acids in selenium chickpea sprout s.

测定分析富硒鹰嘴豆芽氨基酸含量,采用氨基酸自动分析仪测定富硒鹰嘴豆芽中氨基酸含量的方法,分析比较氨基酸的含量和比例。

To determine and analyze the contents of amino acid s in selenium chickpea sprouts.

测定分析富硒鹰嘴豆芽氨基酸含量,采用氨基酸自动分析仪测定富硒鹰嘴豆芽中氨基酸含量的方法,分析比较氨基酸的含量和比例。

In part four, a new method of protein assay based on charge-transfer reaction of protein with chloranil has been established.

第四部分,作者首次建立了以四氯苯醌荷移反应测定蛋白质的新方法,并测定了蛋白质结合四氯苯醌的最大结合数和反应平衡常数。

Local lesion assay indicated that when mixed with TMV-infected leaf juice dilution within 3 minutes, 5 disinfectants showed 100% inactivation ratio, including 30% chloride of lime 1:9 solution, 98% trisodium phosphate 1:9 solution, 7% sodium hypochlorite 1:150 solution, anonymity disinfectant used in USA 1:125 solution, and 75% alcohol solution.

摘 要:为了指导烟草漂浮育苗中剪叶工具消毒剂的使用,采用半叶法测定了9种药剂对烟草病叶汁液中TMV的钝化效果,采用叶面喷雾法测定了药剂对烟草的药害浓度与药害症状。

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