测定

Methods MSCs were obtained from tibias by density gradient centrifugation and were tested the rate of adhesion. The configuration was observed under phase-contrast microscope consecutively and the cell growth was measured by MTT method. Cell cycle and surface antigens were detected through flow cytometer.

测定其接种贴壁率，在倒置显微镜下连续观察细胞的形态变化，利用甲基噻唑基四唑（methyl thiazdyl tetrazolium， MTT）法测定 MSCs 生长曲线，并应用流式细胞术测定细胞表面标记物。

A procedure of high-density exposure was developed to study the gill microenvironment of Neon Tetras.

建立了测定小个体鱼种鳃部微环境特殊条件的高密度暴露方法，利用这种方法测定了彩虹方头鱼鱼鳃微环境的pH、碱度、粘液含量等参数，并根据测定结果计算了鱼鳃微环境中铜的形态分布。

So it was primarily identified as Bacillus subtilis. In the antifungal activity test,the method of sitzkrieg culture, asynchronism culture and hyphal extension inhibition assay of plate were adopted to study the inhibitive effect of the antagonist on Fusarium graminearum, and at the same time,the test conditions were explored.

在拮抗物质抗菌活性测定的研究中，分别采用对峙培养法、异步培养法和杯碟测定法研究枯草芽孢杆菌对小麦赤霉菌的抑制作用，探索抗菌活性测定的条件。

Methods:(1) bFGF was loaded with PLGA, PELA and gelatin microspheres. The pharmac characteristics of particle size distribution, loading efficiency, entrapment efficiency, bFGF release kinetics were evaluated with scanning electronic microscope, MTT and ELISA respectively. The prescription with the best pharmac characteristics and the highest bioactivity of bFGF was chosen for the next step.

（1）制备PLGA，PELA和明胶微球，通过扫描电镜和激光粒度分布仪测定其粒径分布；ELISA法测定微球溶出度，包封率，bFGF释出动力学；MTT法测定微球释出的bFGF的生物活性，筛选出一种具有较好药剂学性质和较高bFGF生物活性的微球载体材料。

The determination of industrial zinc sulphide content by redox titration method was introduced.

利用氧化还原滴定法测定工业硫化锌的含量，分析了影响硫化锌含量测定的因素，样品测定实验结果表明，该法样本合并标准偏差s_q=0。

We killed some mice at the day 6th , the others were continue to expose to formaldehyde and observe their estrous cycle. HE staining was used to study the pathological changes happened in ovarian tissue. Uranium acetate and lead acetate double staining was used to study the ultrastructure of oocyte. E2 and LH level in blood serum was measured by radioimrnunoassay reagent kit.

第6d处死一批小鼠，用HE染色观察小鼠卵巢组织的病理改变，用醋酸铀—醋酸铅双重染色电镜下观察小鼠卵母细胞超微结构的损伤；用GSH测定试剂盒测定小鼠卵巢组织中的GSH含量；用E_2和LH放射免疫分析试剂盒分别测定小鼠血清中E_2及LH水平；采用超排卵法，计数超排卵数并收集卵母细胞，于油镜下观察生殖细胞遗传物质的损伤。

Currently, the anti-influenza antibody is measured by the hemagglutinin- inhibition test. Although it is simple, but the result is easily influenced by the quality and species source of the red blood cells used. Antibody titer determined by HI test cannot reflect the protection titer either.

血球凝集抑制试验(hemagglutinin- inhibition test，HI test)是一至今仍普遍使用来测定抗流行性感冒病毒抗体的方法，此方法虽然快速、简便，但测定结果容易因为红血球的品质及来源而受影响，且血球凝集抑制试验所测定出的抗体效价亦不能反应真正的保护能力。

The markers usually used in the molecular epidemiologic study includes internal dose marker, Ames test, DNA adducts, and cytogenetic markers, e.g. chromosome abeeration, sister chromatid exchange and micronucleus.

本文讨论目前常用的生物侦测方法，包括内在剂量测定，致癌物—DNA结合物的生物有效剂量测定及生物效应的测定。

Methods To test the content of environmental mercury in the treated region of Songhua river and the control region; to test the content of mercury in hair of children 10～13 years old and to determine the growth index such as height, body weight, sitting height, chest circumference, back strength, grip strength; to measure the IQ with CRT-RC.

对松花江汞污染综合治理地区与无污染的对照地区环境中的汞含量进行测定；选择2个地区10~13岁儿童测量其发汞含量并进行儿童生长发育指标身高、体重、坐高、胸围、背肌力、握力的测定；用联合型－瑞文测验的方法进行智商测定。

Objectives:To introduce the author's experiences in bioassay of platelet activating factor with thin layer chromatographic separation and platelet aggregation method,and to discuss probable problems in the process.

目的：介绍作者应用薄层层析分离和生物测定法测定血小板激活因子的体会，重点讨论分离和测定过程中可能出现的问题及注意事项。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。