测定

After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.

实验评估：手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量；应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平；以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜；以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。

Image analysis system was used for semi-quantitive assay of TGF-βRⅠ and R Ⅱexpression, and microphotography was used to record the morphologic changes of culturedchondrocytes. The results are:1. TGF-〓 showed sequentially inhibitory and promotive effects on the proliferation of human articular chondrocytes of two week monolayer culture from one passage to eight passage.

本研究采用CellTiter 96〓单溶液细胞增殖分析试剂盒测定体外培养中的软骨细胞的增殖活细胞数量；免疫组化法测定软骨细胞TGF-βR Ⅰ、R Ⅱ，Ⅱ型胶原和S-100蛋白的定性表达；酶免分析法测定TGF-〓作用后的Ⅱ型胶原定量表达；通过图象分析仪，对TGF-βRⅠ、R Ⅱ的免疫组化染色结果进行半定量分析；本研究的实验结果如下：1。

In this article, three kinds of prealloy powder were developed by water atomization method. The base attribute, pattern, phase composition, differential thermal analysis of powder were researched. Sintered density, hardness, bend strength, Young's modulus were researched as hot pressed sintering temperature changing. By using abrasive wearing equipment upbuilt by the authors, the wear resistance were determined. Green-pressing density, simulated green-pressing strength and bonding momental of high frequency brazing were assayed. SEM corrosion structure and SEM appearance of fracture were observed.

本文采用水雾化法开发了三种预合金代钴粉末：对粉末的基本属性、形貌，粉末的相组成，粉末的差热性进行了分析研究；测定了它们的烧结密度、硬度、抗弯强度、弹性模量随热压烧结温度变化的规律；采用作者自行建立的磨料磨损试验方法，测定了它们的质量磨损百分率；测定了它们的冷压生坯密度、模拟生坯强度以及高频钎焊的结合力矩；观察了热压烧结SEM腐蚀组织和SEM断口形貌。

Then, the multidentate amine immobilized on silica gel was used as ligand for CuCl in ATRA of CCl_4 with MMA and methyl trichloroacetate with St repeatly. The conversion and purity of the product were determined through the gas chromatography and ~1H NMR respectively. Finally, the multidentate amine immobilized on silica gel was used as ligand for CuCl in ATRP of St initiated by TCPP and MMA initiated by MMTCB. The number average molecular weights of the polymers were determined through GPC.

此多齿胺改性硅胶配体被分别多次重复用于四氯化碳与甲基丙烯酸甲酯，三氯乙酸甲酯与苯乙烯的ATRA反应中，通过气相色谱测定了原料的转化率随时间的变化，且通过核磁~1H NMR测定了产物的纯度，我们还进一步将此种多齿胺改性硅胶配体用于TCPP引发St的ATRP和MMTCB引发MMA的ATRP反应中，通过GPC测定所得聚合物的分子量。

The testing methods and targets were shown as below:Recorded milk weights from the 7th day postpartum to 15th day postpartum,and calculate the average day-milk yield;Milk samples were draw in 7th day and 14th day postpartum,and testing the milk fat,milk lactose,milk protein,milk Zn and the milk somatic cell counts;Blood samples were adopted at the 7th day postpartum,testing the levels of serum PRL; Blood samples adopted at the 1st day and the 12th day in oestrous cycle were used to test the levels of E_2 and P_4 in serum;In the experimental stage, registering the condition of parturition, the mean birth weight of calves,the first oestrus,the oestrus scycle postpartum and the conception rate of the first service after artificial insemination.

试验方法及所测指标如下：于产后第七天到第十四天记录日产奶量，最后计算每组平均日产奶量；每组随机抽取分娩后第七天、第十四天的奶样，测定乳糖、乳蛋白、乳脂、乳锌含量及乳体细胞数；于产后第七天，随机采取奶牛血样，测定血清中PRL含量；于发情周期的当天和第12天，随机采取血样，测定血清中雌二醇(Estradiol，E2)和孕酮(Progesterone，P)的浓度；记录奶牛的分娩情况、犊牛初生重、产后第一次发情日期、产后发情周期，并采用人工授精技术进行受精，记录第一情期受胎率，最后取平均值，作为衡量奶牛繁殖性能的指标。

A small oscillogram and a new method of oscillographic determination based on it were pre- sented.

研究表明，利用小示波图不仅能使在经典示波图端点附近不易准确测定的物质得以测定，还可以在宽广的电位窗口内灵活、稳定、灵敏、高选择性地进行示波测定。

In this paper, Neural Network and Combined Wavelet Transform with Neural Network for OCPD are studied systematically. It also eliminated the artificial influence in oscillographic determination, enhanced the automatization degree of OCPD.

本论文对神经网络及其与小波变换相结合用于示波测定进行了系统的研究，较好地消除示波测定中人为误差的影响，提高了示波测定的自动化程度，全文共分四章。

To investigate the relationship between the impulse oscillometry method and plethysmogrophy used in measuring airway resistance,Using the two methods to measure the airway resistance and the ventilation data of 158 patients.

为探讨脉冲振荡法和体描法测定呼吸道阻力的关系，对158例患者分别用脉冲振荡法和体描法测定呼吸道阻力，同时测定测试患者的通气功能，对2种方法测得的呼吸道阻力进行相关分析，考察各组数据的分布情况。

Objective: To measure the variation and significance of respiratory impedance and pulmonary ventilation function in patients with bronchial asthma by impulse oscillometry before or after the bronchial provocation test, investigate the relationship between the IOS method and bronchial provocation test, and evaluate the clinical significance in bronchial asthma by IOS method.

目的：应用脉冲振荡法测定支气管哮喘患者在支气管激发试验前后的呼吸阻抗及肺通气功能参数的变化规律和临床意义，探讨IOS测定方法能否作为支气管激发试验的判断方法以及IOS测定参数在哮喘诊断中的价值。

The oxidable potential difference of DA and AA was about 184 mV at Poly-Evans Blue modified electrode. So it is used to simultaneously determine the DA and AA without any interference. The interference of other components could be efficiently eliminated at the modified electrode, and this method can be used for determination of DA and AA in real sample with satisfactory results.

当DA与AA共存时，由该修饰电极检测的二者氧化峰电位差达184mV，故可同时测定DA和AA，并有效消除其它组分对DA测定的干扰，已用于实际样品中DA和AA含量的测定，结果令人满意。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。