测定

The level of PGE was assayed by spectrophotometer; Meanwhile, 3H-TdR incorporation method was used to detect ConA- and LPS- induced splenocytes proliferation and the production of interleukin-1 and IL-2. The content of antibodies to CII was determined by ELISA method. Pathological examination of articulus tissue was observed by Hematoxylin-eosin stain.

检测小鼠足爪肿胀度并对炎症进行评分；测定胸腺指数和脾脏指数；~3H-TdR参入法检测胸腺、脾淋巴细胞增殖反应；~3H-TdR参入法测定白细胞介素-1(interleukin-1，IL-1)和IL-2水平；ELISA法测定小鼠血清中抗CⅡ抗体含量；分光光度法检测足爪局部产生的前列腺素水平；HE染色法对关节组织作病理检查。

Based on the absorbance change of indicators with the concentration of hydrogen ion released from the enzyme-catalyzed reaction, a convenient colorimetry method is established for the assay of acidic phospholipase 〓 and glycogen phosphorylase b Brilliant yellow and bromothymol blue are chosen as indicators for assays of acidic phospholipase 〓 and glycogen phosphorylase b by following the absorbance changes at 495 nm and 615nm respectively The method is simple, sample-saving, sensitive and valid for a wide range of enzyme concentrations.

为了研究糖原磷酸化酶的激活动力学和酸性磷脂酶〓的复性过程，我们根据酶催化反应中释放氢离子浓度的变化引起相应的指示剂的光吸收发生变化的原理，建立了一种简捷的比色法，用于测定酸性磷脂酶〓和糖原磷酸化酶b的活性。选用亮黄和溴麝香草酚兰分别作为酸性磷脂酶〓和糖原磷酸化酶b测活的指示剂，在495 nm和615 nm处检测二者的光吸收值的变化，可以测定酶活。本方法的优点是，可在比较宽的酶浓度范围内进行活力测定，而且操作简单、节省样品、灵敏度高。

Patients with a body mass index≥25㎏/㎡ and patients with cardiac cachectic, hypertension, diabetes, hyperthyroidism, hepatorenal disease, cerebral vascular disease, pulmonary embolism ,malignant tumor ,connective tissue disease and infective disease were excluded from the study.

空腹血糖采用氧化酶法测定；应用ELISA法测定血浆leptin、INS及TNF-α水平；胰岛素抵抗指数为FPG与空腹血浆胰岛素的乘积除以22.5，然后取自然对数，即Homa-IR=㏑（FPG×FINS/22.5）；应用改良Simpson法测定LVEF。

A new method to determination of trace water content in battery material by means of differential scanning calorimetry was introduced.

介绍了利用差示扫描量热法测定电池材料中微量水分的新方法，它是基于在适当条件下，测定蒸馏水及材料中水分的蒸发热，从而间接测定材料的含水量。

With the observation of the outward appearances, it can be known roughly that if the sample was added with adulterations. Since the beeswax dissolves in the benzene but the adulterations, such as gypsum, starch and so on do not, these can be determined. By the verification of acid value and alcohol dissolve ability, the acid or non acid added can be determined. By the change of saponification value, the ester or non ester added can be determined. By the carbonification degree in the concentrated sulphuric acid, the mineral wax, such as paraffin wax added can be determined.

通过样品外观的观察初步定性是否掺有杂质；利用纯蜂蜡在苯中溶解，而掺入的石膏、面粉和淀粉等杂质在苯中不溶而定量鉴定这类杂质；由酸值变化和乙醇溶解物的量，测定掺入的硬脂酸等酸性物质或其它的非酸性物质；由皂化值变化，定量测定掺入的动物脂等酯类物质或其它的非酯类物质；根据蜂蜡中的烃类在浓硫酸中可被炭化，加入的石蜡等矿物蜡不被炭化来测定此类掺杂物。

The main function of sucrose is to maintain normal respiration, so it can only be used to join the preservative, and make a good effect in maintaining the water balance of Dianthus Caryophyllus when dealing with 0.01% Phytic Acid +2% S +200 mg/L8-HQC solution; Finally, in the mensuration of Dianthus Caryophyllus fresh weight change, no matter using which the vase planting solution, the fresh weight of Dianthus Caryophyllus increases in the initial phase and then gradually decreases.

结果表明：在花径变化的测定中，经0.01%植酸+2%S+200mg/L8-HQC溶液处理的香石竹在保鲜期间得到最好的保鲜效果，切花的生长状况达到最佳状态；在水分平衡的测定中，结果表明蔗糖溶液对香石竹的水分平衡的保持有负作用，蔗糖的作用主要作用是维持正常的呼吸作用，因此它只能用来加入到保鲜剂中，处理0.01%植酸+2%S+200mg/L8-HQC在保持切花的水分平衡上起到较好的效果；最后在香石竹的鲜重变化测定中，无论什么瓶插液，香石竹切花的鲜重在初期阶段增加，然后逐渐减少，水分的吸收与丧失会有波动，但总趋势是失水量大于吸水量。

Choosing 50 patients that met the standard of catabatic period after treatment.We also survey the lung-function and the EOS count of peripheral blood of the 50 patients.Comparing with the data of two groups.

对57例急性发作期哮喘患者做肺功能测定和取外周血进行EOS的计数，经后选取符合缓解期标准的患者50例，也做肺功能测定和外周血EOS的计数，比较两期患者肺功能及EOS测定结果。

Methods Choosing 57acute-outbreak asthma sufferers,then checking out the lung-functions and taking the EOS count of peripheral blood.Choosing 50 patients that met the standard of catabatic period after treatment.We also survey the lung-function and the EOS count of peripheral blood of the 50 patients.

对57例急性发作期哮喘患者做肺功能测定和取外周血进行EOS的计数，经后选取符合缓解期标准的患者50例，也做肺功能测定和外周血EOS的计数，比较两期患者肺功能及EOS测定结果。

Methods Choosing 57acute-outbreak asthma sufferers,then checking out the lung-functions and taking the EOS count of peripheral blood.Choosing 50 patients that met the standard of catabatic period after treatment.We also survey the lung-function and the EOS count of peripheral blood of the 50 patients.

对57例急性发作期哮喘患者做肺功能测定和取外周血进行EOS的计数，经治疗后选取符合缓解期标准的患者50例，也做肺功能测定和外周血EOS的计数，比较两期患者肺功能及EOS测定结果。

Methods Choosing 57acute-outbreak asthma sufferers,then checking out the lung-functions and taking the EOS count of peripheral blood.Choosing 50 patients that met the standard of catabatic period after treatment.We also survey the lung-function and the EOS count of peripheral blood of the 50 patients.Comparing with the data of two groups.

对57例急性发作期哮喘患者做肺功能测定和取外周血进行EOS的计数，经治疗后选取符合缓解期标准的患者50例，也做肺功能测定和外周血EOS的计数，比较两期患者肺功能及EOS测定结果。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。