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Methods: Mice bearing in vivo transplanted tumor-H22 hepatic carcinoma were used to evaluate the drug"s activity of anti-tumor and GAPSGF"s efficacy enhancing effect by calculating the ratio of antitumor and q. HE dyeing was applied to observe the tumor"s morphological changes after the anti - tumor drug. It is observed the change of theperipheral white blood cells in serum and the body weight to analyze the GAPSGF"s toxicity reducing effect on the anti - tumor drug-Cytoxan. Applying ELISA method to observe the change of IL-2 in serum ,Applying carbon clearance method to test the function of uninuclear phagocyte,took the weight of the spleen and thoracic gland, analyzed the mechanism — efficacy enhancing and toxicity reducing of GAPSGF.

构建体内动物移植性H_(22)肝癌模型,通过计算抑瘤率与q值来分析药物的体内抗肿瘤活性,判断树舌多糖GF注射液的增效作用;采用瘤组织常规HE染色观察药物作用下的肿瘤细胞形态变化;观察外周血WBC数和体重改变来分析树舌多糖GF注射液对环磷酰胺抗肿瘤的减毒作用;采用ELISA法测定血清IL-2、小鼠碳粒廓清法测定单核吞噬细胞功能、取胸腺与脾脏称重测定脏器指数等以分析树舌多糖GF注射液增效减毒的作用机制。

On the basis of determining their resistant spectrum, CY21, CY23, CY27, CY29, CY30, CY31, CY32, Su-11, Su-14 were used to investigate the resistant components at seeding stage, such as latent period, infection efficiency, uredinium length, sporulation capacity, period of spore production and severity. Also, two equal weight races' mixture CY27:CY30, CY27:CY31, CY27:CY32, CY31:Su-4, CY30:Su-11 were continued to reproduce six generations of Libellula and Xiannong 4 respectively, and the ration changes of each race were tested.

在抗性谱测定的基础上,采用CY21、CY23、CY27、CY29、CY30、CY31、CY32、Su-11、Su-14等9个条锈菌菌系,分别测定其苗期潜育期、侵染频率、夏孢子堆长度、产孢强度、产孢期以及严重度,同时将CY27:CY30、CY27:CY31、CY27:CY32、CY31:Su-4、CY30:Su-11等5个等量混合菌系组合分别在里勃留拉和咸农4号上继代培养6代,测定各小种在组合中的比例变化。

For NIRS quantitative analysis, three quantitative models were built. They were three quantitative models of norflocaxin capsules from different manufacturers, the bi-component quantitative models of pulvis fellis ursi and the quantitative model of scutellaria.

对于近红外定量分析,建立了三个定量模型,分别是:三种多厂家诺氟沙星胶囊诺氟沙星胶囊定量测定模型、熊胆双组分定量测定模型和黄芩中黄芩苷定量测定模型,并对各模型的专属性和准确性进行了评价。

The physicochemical properties, fatty acid composition and mineral elements of Viburnum cylindricum seed oil were determined. The results showed that 20 kinds of fatty acids in the Viburnum cylindricum seed oil were identified, in which the main components were oleic acid (33.34%), 8,11-octadecadienoic acid (28.24%) and palmitic acid (22.55%); the seed oil contained 6 kinds of essential mineral elements including Ca, P, Mg, Fe, K and Mn, and their contents were in the reasonable range of body requirement.

测定了水红木油的理化指标;用GC/MS法测定了脂肪酸组成及含量,共检出20种脂肪酸,主要成分9-十八碳烯酸占33.34%,8,11-十八碳二烯酸占28.24%,十六烷酸占22.55%,3种脂肪酸共占84.13%;用ICP-OES法测定了矿质元素的种类和含量,检出的Ca、P、Mg、Fe、K、Mn 6种矿质元素均为人体必需元素,含量均在人体所需合理范围内。

Degree of deacetylation of the three different chitosan are determined by acid -base titration or acid -base conductometric titration.The MW of chitosan I and II by viscometry is 1.0 X 106and 9.5 X 105,respectively.

分别采用酸碱指示剂滴定和电导滴定法测定了壳聚糖的脱乙酰度,后者测定结果大于前者;应用粘度法测定原料壳聚糖Ⅰ和Ⅱ的粘均分子量分别为:1.0×10~6、9.5×10~5。

The Zetasizer Nano ZS is made in Marvern in England. It is the newest Zetasizer Nano ZS with the newest technology. Malvern Zetasizer Nano ZS90 is for the measurement of size, zeta potential of dispersed particles and the molecular weight of random coiled polymers. If it connect with the Autotitrator, it can measure zeta potential as a function of pH, conductivity or concentration of an additive, provides much greater insight into the processes involved in stabilizing or flocculating disperse systems.

英国马尔文公司生产的Zetasizer Nano ZS型纳米粒度仪是目前世界上技术最先进的纳米粒度仪之一,该仪器可以测定纳米材料的粒度分布、ZETA电位值,并可以进行蛋白质的熔点测定,及蛋白质等大分子和聚合物材料的分子量测试,如果与自动滴定仪相连接后,可自动测定粒度或Zeta电位随pH值、盐浓度或添加剂浓度等的变化趋势。

Four testing methods to detect the pollen viability of cucumber were being compared. The results indicated that I-KI staining method and acetic red dyeing were not suitable to judge the pollen viability of cucumber. Tetrazolium method can be used but the error is oversize. By and large, the Germination in vitro method was an efficient, stable and simple way to judge the pollen viability of cucumber. The component of culture medium is 10% sucrose+ 100mg/L H_3BO_3. If inoculated intraday, collect pollen of fresh abloom flower at 6:00 and if inoculated at the second day, collect before dark.

比较了4种常用的花粉活力测定方法——I-KI染色法、醋酸洋红染色法、TTC染色法和离体萌发法,认为I-KI染色法和醋酸洋红染色法不适于测定黄瓜花粉生活力,TTC染色法虽适用但误差较大,综合考虑各项因素,确定离体萌发法是最准确、简便、稳定的测定黄瓜花粉活力的方法。

According to the principle of MeasureMent of pH of solutions by the Method of electrical potential,an accurate calculation forMula of the real value of pH of solutions was deduced for electrode's electrical potential and the reading of acidoMeter.

根据电位法测定溶液pH值原理,由电极电位和酸度计指示测定值,导出待测溶液实际pH值准确计算式,消除了S标度≠S电极时产生的测定误差,并用实验进行了验证和样品分析。

Methods: four male volunteers were recruited to live in a disabled submarine simulator which is close, dark and algid for 10 days. they were supplied 1.5 l water and one portion of saving life dry provision everyday. the body weight was detected before the experiment and on day 3, day 4, day 5 and day 10 after exposure. at the same time, venous blood taken suction from elbow was used to detect the level of blood glucose, which was centrifuged to take the upper serum to detect the level of ffa at low temperature by colorimetric.

4名男性受试人员在封闭、黑暗、寒冷的试验舱体中进行为期10 d的模拟失事潜艇环境实验,每人每天供水1.5 l和1盒救生压缩干粮,实验前和第3、4、5、10天出舱的晨起测定体质量,抽取肘部静脉血,测定血糖;低温离心留取上层血清,采用一次提取比色法测定游离脂肪酸。

In order to provide some scientific data for judging biological toxicity and bioavailability caused by heavy metals in sediments in Guilin, China, for the first time, the authors sampled sediments in different water areas in Guilin, and determined AVS by P-Amino dimethyl aniline dihydro chloride photometry through a set of acidification-ventilation-absorption AVS reaction unit, determined copper, lead, zinc, cadmium and nickel with tomic absorption spectrophotometry, mercury and arsenium with atomic fluorescence, and cipangopaludina cathayensis was selected as the tested species for bioavailability experiment.

首次以桂林水系沉积物为研究对象,通过酸化-吹气-吸收装置,用对氨基二甲基苯胺光度法测定沉积物中AVS含量,用原子吸收分光光度法测定SEM和间隙水中Cu、Pb、Zn、Cd、Ni含量,用原子荧光法测定Hg、As含量。

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