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Eckenwalder Lab, Department of Botany is primarily dedicated to research in woody plants including biosystematics, chemotaxonomy, numerical taxonomy and morphometrics, paleobotany, and cladistics, accommodation of hybridization in phylogenetic analysis, biogeography, systematics and reproductive biology of convolvulaceae.

植物学系Eckenwalder研究室主要致力于木本植物的研究,其研究领域包括生物分类学,化学分类学,数量分类学和形态测定,古植物学和遗传分类学,系统分析中杂交调节,生物地理学,滕本植物的分类学和生殖生物学等。

Methods BALB/c mice were inoculated muscularly with HIV-2 gag recombinant DNA vaccine, or recombinant fowlpox virus, or both.

大量制备并纯化HIV-2 gag重组DNA疫苗和重组鸡痘病毒,以肌肉注射的方式免疫BALB/c小鼠,ELISA法检测小鼠血清HIV-2抗体,流式细胞仪测定CD4^+、CD8^+T淋巴细胞亚类数量,乳酸脱氢酶释放法检测脾CTL对HIV-2靶细胞的杀伤活性。

The white-cell count in peripheral blood,transformation rate of lymphocytes and phagocytosis efficiency of macrophages in spleen were measured in redfin puffer weighing 181.75±17.21 g starved for 0d,10d,20d,30d,and 40 d.

对红鳍东方鲀Fugu rubripes进行正常投喂和饥饿处理(10、20、30、40 d)后,测定红鳍东方鲀外周血中白细胞数量以及脾脏中淋巴细胞的转化率和巨噬细胞的吞噬率。

E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.Results:Myocardial collagen in model group increased obviously compared with normal group from the results of electron microscope、H.E、Sirus red in supersaturated picric acid solution.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型,随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组,并设正常对照组,用药四周后处死动物通过透射电镜观察心肌细胞超微结构;HE染色、苦味酸天狼星红染色观察心肌胶原数量;放免法测定血清透明质酸的含量;免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

Methods:Establish the model of myocardial fibrosis in chronic stage of viral myocarditis by injecting CVB3 into Balb/c rats,divide these rats into model group、Tong xin luo low dose group、Tong xin luo medium dose group、Tong xin luo high dose group and set up normal group,after four weeks of giving medicine execute the animals and observe the ultrastructure of myocardial cell with tramission electron microscope,the quantity of myocardial collagen was observed by staining with H.E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型,随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组,并设正常对照组,用药四周后处死动物通过透射电镜观察心肌细胞超微结构;HE染色、苦味酸天狼星红染色观察心肌胶原数量;放免法测定血清透明质酸的含量;免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

Microbial population changes in farmland soil of different aged H. lupulus was investigated, and microbial number and diversity index in soil of different aged H.

通过测定22,20,18,8和5年啤酒花农田土壤微生物区系的变化,分析了不同植龄土壤微生物数量及多样性指数与啤酒花产量和品质的关系。

Comparison on the difference of the growth of Haematacoccus pluvialis strain 797 in five different culture media SM, BG-11, BBM, MCM, SE on cell density, A674, pH, dry weight and astaxanthin content of the algae.

通过测定雨生红球藻的细胞数量、A674值、培养液pH、藻粉干重、虾青素含量比较了雨生红球藻797株在5种不同培养基SM、BG-11、BBM、MCM、SE中的生长情况。

Methods The animal model of skin avulsion injury was replicated, and the expression of CD18mRNA in avulsed skin tissues was detected by RT-PCR method; and the amount of neutrophilic granulocyte in tissues was detected by MPO method in order to analyze the tendency of neutrophil of avulsed tissues after trauma.

复制猪皮肤撕脱伤模型,采用RT-PCR方法检测伤后不同时间撕脱组织中CD18mRNA的表达;用髓过氧化酶(Myel operoxi daBe enzyme,MPO)法测定组织中中性粒细胞数量,研究伤后撕脱组织中中性粒细胞的趋化情况。

Through calculations, the characteristics of all kinds of edges can be decided by comprising the values of EH.

这两个公式都是从群落的种群数量和结构上来测定各种边缘效应的强度。

By use of electrolytic pure iron ,the melts containing different content of heterogeneous nucleus were made in a Mo wire furnace.

采用电解纯铁通过高温钼丝炉制备含有不同数量异质核心的试样,用差热分析法分别测定了纯铁和高洁净钢试样在相同冷却速度下的过冷度。

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