测光法

The application of reflected optical difference system in surface crack of aluminum detection is also discussed. By analyzing the experimental data, we found that the reflected pulsed wave techniques could be used to measure the position of crack precisely and depth imprecisely. Transmitted wave contain much size information of the crack, despite without position information. The spectrum of transmitted is sensitive to the depth of crack. The deeper the crack is, the narrower the spectrum bandwidth is.

使用自建的光偏转差分检测系统，对铝样品中多种表面缺陷进行了检测，对表面波直达波，反射波，透射波波形及频谱进行了分析，讨论超声表面波与各种缺陷相互作用后的变化，通过对实验结果的分析，发现缺陷脉冲发射法可以精确测定缺陷位置，粗略估计缺陷深度；而透射法测的波形无法定出缺陷位置，但包含了缺陷大小信息，透射波频谱对缺陷深度非常敏感，随缺陷深度的加深，频谱不断的左移。

This paper is a report of a light frequency equation of inflexion on Planck function we have deduced in light of Planck formula.

基于普朗克公式，利用微分方法导出了普朗克函数的拐点光频率方程，并通过牛顿迭代法求出了这个方程的两个根，进而找到了可求任意温度下两个拐点光频率的普遍公式，为研制热辐射测温仪工作波长的选择提供了技术参考。

The ee is assessed by chiral GC and/or polarimetry.

对映异构体过量值由手性气相色谱法和/或旋光测定法测得。

Analyses show that refractive index changeinduced by thermal effect is similar to the change induced by other effects for linearabsorption,if laser pulse width is far less than the characteristic time of sample,i.e.refractive index change is directly proportional to spatial part of incident pulse laserintensity,so time averaged nonlinear refractive index can be defined.

结果表明，当激光脉冲宽度远小于被测样品的特征时间时，对于线性吸收来说，其热效应产生的折射率变化与其它效应产生的折射率变化有类似的形式，即与入射脉冲激光光强的空间部分成正比。因而可定义时间平均非线性折射率。在这种情况下，可用通常的Z-scan法进行非线性折射率及热光系数的测量，所得结论符合以前的实验结果。

This method strengthens the weightiness of the point whose light intensity is high,increases the measuring accuracy.

光切法是近10年发展起来的一种非接触测量方法，其基本原理是用线光源产生的光平面照射被测物体，由于

Off-line HPLC coupled with MS gives the result that 4-chlorophenyl urea and 2-chloro-N-(4-chlorophenyl) benzamide are two photoproducts. In comparison with their retention times and UV specta with those of standards, some other photoproducts identified are 2-chloro benzamide, 4-chlorophenyl urea and 4-chloro aniline.

收集光解产物各级分，采用直接进样法在MS上测得其中的二种光解产物为对氯苯基脲和N-邻氯苯甲酰胺：通过与标样在同样HPLC条件下的保留时间和UV吸收光谱相对照，证明产物中存在有邻氯苯甲酰胺、对氯苯基脲和对氯苯胺等。

Based on the absorbance change of indicators with the concentration of hydrogen ion released from the enzyme-catalyzed reaction, a convenient colorimetry method is established for the assay of acidic phospholipase 〓 and glycogen phosphorylase b Brilliant yellow and bromothymol blue are chosen as indicators for assays of acidic phospholipase 〓 and glycogen phosphorylase b by following the absorbance changes at 495 nm and 615nm respectively The method is simple, sample-saving, sensitive and valid for a wide range of enzyme concentrations.

为了研究糖原磷酸化酶的激活动力学和酸性磷脂酶〓的复性过程，我们根据酶催化反应中释放氢离子浓度的变化引起相应的指示剂的光吸收发生变化的原理，建立了一种简捷的比色法，用于测定酸性磷脂酶〓和糖原磷酸化酶b的活性。选用亮黄和溴麝香草酚兰分别作为酸性磷脂酶〓和糖原磷酸化酶b测活的指示剂，在495 nm和615 nm处检测二者的光吸收值的变化，可以测定酶活。本方法的优点是，可在比较宽的酶浓度范围内进行活力测定，而且操作简单、节省样品、灵敏度高。

The purpose of fogging is to relax accommodation by creating an artificial myopia in all eyes regardless of their original refractive status.

9雾视法的目的在于使被测眼的调节松弛，不论其原来的屈光状态如何，被测眼均处于人为的近视状态。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Methods The methods of hand-feeling, X-ray photography and electricity were applied to the measurement of the root-canal length, and then the outcomes were compared with the solid measuring method of the separated teeth.

采用手感法X光照片，电测法3种方法测量根管长度，并和离体牙实测相比较。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。