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At the one-cell stage, each embryo was injected with gradient doses of 2-8ng MO. The control morpholino, was purchased from Gene-Tools.To test the knockdown effectiveness of the morpholino, the tbx2-EGFP (enhanced green fluorescent protein) construct was generated. And coinjection of morpholino with mRNA tests its specificity of the morpholino. Overexpression of tbx2 via mRNA microinjection helps us understand gaining function of tbx2. Results The tbx2-EGFP construct tests the knockdown effectiveness of the morpholino. Zebrafish embryos lacking tbx2 function have defects in cardiac contractility, rhythm and morphology in a dose-dependent manner. The embryos with c-MO are normal in development. Coinjection of morpholino with mRNA rescue the phenotype of tbx2 knocking down. The phenotypes include pericardial edema, hypogenetic ventricle, dilation of atria, arrhythmia, bradycardia, asystole, abnormal atrioventricular canal, aberrant valve and blood regurgitation.

结果荧光蛋白融合标记实验验证了tbx2-MO可以很好得阻抑斑马鱼胚胎tbx2的表达,tbx2-MO组胚胎在心脏收缩性、节律和形态学发生上出现了异常,心脏畸形随着注射剂量的增加而出现比较一致的表型,而c-MO组胚胎没有出现明显的畸形;tbx2-mRNA和tbx2-MO的共注射减少了tbx2-MO胚胎心脏畸形的发生率及减轻了心脏畸形的程度,验证了所设计合成的tbx2-MO对tbx2基因的抑制作用具有特异性;tbx2-MO组胚胎心脏缺陷包括心室发育不良、心房扩张、房室管和瓣膜异常以及心率缓慢、心律不齐、心脏停搏、血液返流等,并根据心脏发育异常的程度分为轻、中、重度畸形组tbx2-mRNA基因过表达胚胎没有见到心腔的形成。

And after analyzing the heterogeneity of sand body, point the minor channels, especially, have important influence on the intraformational oil-flooding direction, forming and distribution of remaining oil because of their function as barrier, breaking fluid and poor permeability in fluvial sand body.

通过非均质性研究,进一步揭示了单一河道砂体内部非均质性,特别是次分流河道砂体的分布,在河道砂体内起到遮挡、阻流、特低渗透等作用,对层内水驱油方向、剩余油形成与分布具有重要影响。

Governing equations of nonprimatic channel unsteady flow are simplifiedly analyzed with the expressed form of channel parabolic cross section by variation of distance,and applied with dam sudden release problem in nonuniform wetted perimeter cross section on neglecting friction and slope effects.

利用明渠沿程抛物型断面的概化关系式,对棱柱体明渠非恒定流的控制方程进行简化分析,并应用于忽略底坡和摩阻影响的非单一湿周断面明渠瞬时全溃问题。

According to the difference of sediment transportation by the single wave or wave current and the unidirect flow, two key factors are mainly grasped to consider the influences of waves on the sediment.

根据波浪和波流联合输沙与单向水流输沙特性的不同,本文主要抓住了两个关键因素来考虑波浪对泥沙的作用,即改变底部摩阻和泥沙沉速。

These are body-plant, metre, rear flowboard, bumper, instrument board, door panel, top celling, seat, air intake manifold, radiator water chamber, oil tank, tyre, tube, door and window sealing strip, reduce shake part, frictions, textiles and inorganics materials used in vehicles.

包括车身外板、格栅、后阻流板、保险杠、仪表板、门内板、顶棚内村、座椅、进气歧管、散热器水室、油箱、汽车轮胎、胶管、门窗密封条、减振橡胶制品、汽车摩擦材、汽车用化学纤维、汽车用无机材料等。

First, the mechanism of local head loss is clarified from the theory of plane flow and vortex; and the relationship between local head loss coefficient and barrier-discharge of pile permeable groins is derived.

首先,应用平面绕流与旋涡理论阐明其局部水头损失的机理,导出了桩柱透水丁坝局部水头损失系数与阻水流量的关系式。

Thus the toughness of cement can be strengthened, which is helpful to ensure the success of perforating completion operations.

这种阻裂作用增加了水泥石的韧性,一方面有助于射孔完井作业的顺利完成;另一方面有助于水泥承受复杂的井下应力,进而减少&二次窜流&现象的发生。

The sewer is silted up by foulness, and the waste water is everywhere on the ground.

下水道被脏物淤阻了,污水流得满地都是。

When retracted, the doors form part of the outer duct for fan exhaust air.

当收回时,阻流门将作为风扇向外排气的外部管道的一部份。

When retracted, the doors form part of the outer duct for fan exhaust air.

阻流门将作为风扇向外排气的外部管道的一部份。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。